Using visible and near infrared diffuse transmittance technique to predict soluble solids content of watermelon in an on-line detection system

Sugar content is one of the most important factors determining the eating quality of watermelon fruit. In order to detect the fruit soluble solids content (SSC) on-line, this work develops a nondestructive on-line detection prototype system using visible and near-infrared (Vis/NIR) technology. For the acquisition of the diffuse transmittance spectrum of watermelon, the conveyor was set at a speed of 0.3 m/s and ten 150 W tungsten halogen lamps were used as the light source. The crucial model for SSC value prediction was optimized by chemometrics. Partial least squares regression (PLSR), stepwise multiple linear regressions (SMLR), Monte-Carlo uninformative variable elimination (MC-UVE) and genetic algorithms (GA) were applied to the spectra in the range of 687–920 nm. The data pre-processing methods were optimized to transmittance spectra with baseline offset correction (BOC), and the BOC-MC-UVE-SMLR calibration model was the best with a correlation coefficient (r_pre) of 0.70, root mean square error of prediction (RMSEP) of 0.33 °Brix for the prediction set. In on-line testing of 30 samples, the r_pre was 0.66 and RMSEP was 0.39 °Brix. The results showed that a nondestructive on-line SSC value determination prototype based on Vis/NIR technology was feasible.     

西瓜枯萎病抗性及其与体内一些生化物质含量的关系

研究表明西瓜幼苗体内多酚氧化酶、过氧化物酶活性与西瓜枯萎病的抗性间不存在显著相关 ;而抗坏血酸氧化酶活性与西瓜枯萎病抗性之间存在极显著相关 ,这可作为鉴定和筛选抗病性个体的生化指标。西瓜幼苗根系中苹果酸含量、草酸含量及有机酸总量与抗病性不存在显著相关 ;而乙酸和柠檬酸含量则与西瓜枯萎病抗性之间存在着显著和极显著相关。它们亦可作为筛选抗性的生化指标。     

亚低温条件下外源褪黑素和Ca2+对西瓜幼苗生理特性的影响

以西瓜品种8424种子和幼苗为试材,利用人工气候室进行亚低温处理(昼/夜18℃/12℃)20 d,研究外源褪黑素(MT)和Ca²⁺浸种处理对亚低温条件下西瓜种子萌发,西瓜幼苗抗氧化酶SOD、POD和CAT等活性,渗透调节物质可溶性糖和脯氨酸含量的影响。结果表明,亚低温处理的西瓜种子发芽率和发芽势仅为46.5%和40.5%,外源100μmol·L^-1褪黑素和5 mmol·L^-1Ca²⁺复合浸种处理西瓜种子发芽率和发芽势分别达到62.3%和58.5%。外源褪黑素和Ca²⁺浸种处理显著提高了抗氧化酶SOD、POD、CAT和APX活性,促进了渗透调节物质可溶性糖和脯氨酸的积累,有效缓解亚低温对西瓜种子萌发和幼苗生长影响;褪黑素和Ca²⁺复合浸种西瓜幼苗在出苗第20天时植株鲜质量达到8.21 g·株^-1,达到对照处理的85.5%。综上,外源褪黑素和Ca²⁺能通过提高西瓜幼苗抗氧化酶活性和渗透调节能力等,缓解亚低温的不良影响,促进西瓜幼苗生长。