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Irrigation water disinfestation is an unexplored option for reducing Verticillium dahliae inoculum in water and consequently for more efficiently managing Verticillium wilts in Andalusia. We assessed Suppressive Efficacy (SE; water was infested and subsequently treated) and Preventive Efficacy (PE; previously treated water was subsequently inoculated) of OX-VIRIN®, OX-AGUA AL 25® and Deccoklor® in reducing water infestations by V. dahliae conidia. Five concentrations of each disinfestant, the lowest three being recommended by the manufacturer, were tested in vitro against six V. dahliae isolates. Validation assays were carried out in experiments under natural environmental conditions in spring. The four highest concentrations of OX-VIRIN® (0.8–51.2 mL L−1), the three highest of OX-AGUA AL 25® (46.4–417.5 μL L−1) and the two highest of Deccoklor® (0.375 and 3.75 mL L−1), showed an in vitro-efficacy (SE and PE) of 96.2, 80.0 and 100.0% after 30, 5 to 30 and 15 days respectively. Therefore, recommended concentrations for OX-VIRIN® and OX-AGUA AL 25® showed a greater in vitro-effectiveness than those recommended for Deccoklor®. Assays in natural environmental conditions proved that OX-VIRIN® at the recommended concentration of 3.2-mL L−1, applied every 28 days to water, was the most effective treatment (SE and PE), with a 100% reduction of the average relative viability after 56 days. Other chemical treatments showing high in vitro-efficacy, such as OX-VIRIN® at 0.8 mL L−1 and OX-AGUA AL 25® at 46.4 μL L−1 showed an SE of 99.9% after 14 and 28 days when applied every 28 and 14 days, respectively. However, PE of OX-AGUA AL 25® at 46.4 μL L−1 was only 59 and 38% after 28 and 14 days respectively, depending on the experiment. 相似文献
63.
From regionally predictable to locally complex population structure in a freshwater top predator: river systems are not always the unit of connectivity in Northern Pike Esox lucius
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Dorte Bekkevold Lene Jacobsen Jakob Hemmer‐Hansen Søren Berg Christian Skov 《Ecology of Freshwater Fish》2015,24(2):305-316
Contemporary genetic diversity is the product of both historical and contemporary forces, such as climatic and geological processes affecting range distribution and continuously moulded by evolutionary forces selection, gene flow and genetic drift. Predatory freshwater fishes, such as Northern Pike Esox lucius, commonly exhibit small population sizes, and several local populations are considered endangered. Pike inhabit diverse habitat types, including lakes, rivers and brackish marine waters, thus spanning from small isolated patches to large open marine systems. However, pike population structure from local to regional scales is relatively poorly described, in spite of its significance to developing conservation measures. We analysed microsatellite variation in a total of 1185 North European pike from 46 samples collected across both local and regional scales, as well as over time, to address two overarching questions: Is pike population structure associated with local and/or regional connectivity patterns, and which factors likely have the main influence on the contemporary distribution of genetic diversity? To answer this, we combined estimators of population diversity and structure to assess evidence of whether populations within (i) habitats, (ii) drainage systems and (iii) geographical regions are closer related than among these ranges, and whether patterns are temporally stable. Contrasting previous predictions that genetic drift obscures signals of postglacial colonisation history, we identified clear regional differences in population genetic signatures, suggesting a major effect of drainage divides on colonisation history and connectivity. However, several populations deviated from the general pattern, showing that local processes may be complex and need to be examined case‐by‐case. 相似文献
64.
Incubation success and habitat selection of shore‐spawning kokanee Oncorhynchus nerka: effects of water‐level regulation and habitat characteristics
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Changes to water‐level regimes have been known to restructure fish assemblages and interfere with the population dynamics of both littoral and pelagic species. The effect of altered water‐level regimes on shore‐spawning kokanee Oncorhynchus nerka incubation success was evaluated using a comprehensive in situ study in Lake Pend Oreille, ID, USA. Survival was not related to substrate size composition or depth, indicating that shore‐spawning kokanee do not currently receive a substrate‐mediated survival benefit from higher winter water levels. Substrate composition also did not differ among isobaths in the nearshore area. On average, the odds of an egg surviving to the preemergent stage were more than three times greater for sites in downwelling areas than those lacking downwelling. This study revealed that shoreline spawning habitat is not as limited as previously thought. Downwelling areas appear to contribute substantially to shore‐spawning kokanee recruitment. This research illustrates the value of rigorous in situ studies both for testing potential mechanisms underlying population trends and providing insight into spawning habitat selection. 相似文献
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Ignacy Misztal Ignacio Aguilar Daniela Lourenco Li Ma Juan Pedro Steibel Miguel Toro 《Journal of animal science》2021,99(6)
Genomic selection (GS) is now practiced successfully across many species. However, many questions remain, such as long-term effects, estimations of genomic parameters, robustness of genome-wide association study (GWAS) with small and large datasets, and stability of genomic predictions. This study summarizes presentations from the authors at the 2020 American Society of Animal Science (ASAS) symposium. The focus of many studies until now is on linkage disequilibrium between two loci. Ignoring higher-level equilibrium may lead to phantom dominance and epistasis. The Bulmer effect leads to a reduction of the additive variance; however, the selection for increased recombination rate can release anew genetic variance. With genomic information, estimates of genetic parameters may be biased by genomic preselection, but costs of estimation can increase drastically due to the dense form of the genomic information. To make the computation of estimates feasible, genotypes could be retained only for the most important animals, and methods of estimation should use algorithms that can recognize dense blocks in sparse matrices. GWASs using small genomic datasets frequently find many marker-trait associations, whereas studies using much bigger datasets find only a few. Most of the current tools use very simple models for GWAS, possibly causing artifacts. These models are adequate for large datasets where pseudo-phenotypes such as deregressed proofs indirectly account for important effects for traits of interest. Artifacts arising in GWAS with small datasets can be minimized by using data from all animals (whether genotyped or not), realistic models, and methods that account for population structure. Recent developments permit the computation of P-values from genomic best linear unbiased prediction (GBLUP), where models can be arbitrarily complex but restricted to genotyped animals only, and single-step GBLUP that also uses phenotypes from ungenotyped animals. Stability was an important part of nongenomic evaluations, where genetic predictions were stable in the absence of new data even with low prediction accuracies. Unfortunately, genomic evaluations for such animals change because all animals with genotypes are connected. A top-ranked animal can easily drop in the next evaluation, causing a crisis of confidence in genomic evaluations. While correlations between consecutive genomic evaluations are high, outliers can have differences as high as 1 SD. A solution to fluctuating genomic evaluations is to base selection decisions on groups of animals. Although many issues in GS have been solved, many new issues that require additional research continue to surface. 相似文献
69.
为探究青藏高原典型高寒荒漠下垫面水分消耗规律,以2019年青藏高原典型高寒荒漠地区沱沱河站监测的涡动数据和微气象数据为基础,分析不同时间尺度蒸散值的变化特征和下垫面水分消耗特征,研究结果表明:小时平均蒸散发量最大值出现在14—15时,达0.46 mm·h-1;日蒸散值最大值出现在7月7日,为8.58 mm·d-1,最小值出现在4月2日,为0.30 mm·d-1;整个生长季蒸散值呈先增加后减小趋势,其中7月份最大,为120.68 mm,4月份最小,为64.80 mm;生长季总蒸散发量为581.15 mm,平均每天蒸散4.01 mm。整个生长季累计降水量为235.70 mm,蒸散发量与降水量(Precipitation,Pr)的差值(The difference between ET and Precipitation,IETP)显示,2019年青藏高原典型高寒荒漠植被生长季(4—8月)水汽交换以下垫面水分消耗为主。 相似文献
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