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71.
为揭示铜藻(Sargassum horneri)对无机碳利用的基本特性及铜藻在漂浮过程中生物量迅速累积并最终发展为金潮的生理生态学机理,以我国近海金潮原因种铜藻为研究对象,在不同pH (6.5、8.0和9.5)条件下研究了添加TRIS缓冲剂、碳酸酐酶抑制剂(AZ、EZ)和阴离子交换蛋白抑制剂(DIDS)后藻体的固碳速率、光合作用-无机碳响应曲线(P-C曲线)及pH漂移曲线。结果显示,TRIS缓冲剂对铜藻的固碳速率无显著影响(P>0.05),而3种抑制剂均显著(P<0. 05)抑制铜藻的固碳速率且抑制效率从大至小依次为DIDS> EZ>AZ。藻体的固碳速率随着外源无机碳浓度的增加而增加,并渐趋饱和。此外,藻体的最大固碳速率(Vmax)随着pH的升高而降低,而半饱和常数(K0.5)则随着pH的升高而升高,藻体的pH补偿点为9.0左右。结果表明,铜藻既可以利用海水中的CO_2也可以利用HCO_3^-进行光合作用;并且铜藻对HCO_3^-的吸收存在通过阴离子交换蛋白直接转运吸收和利用碳酸酐酶将HCO_3^-转化为CO_2后再吸收两种方式。对HCO_3^-的高效吸收利用能力可能是铜藻保证维持较高光合作用、快速积累生物量并最终演变为金潮的重要因素之一。 相似文献
72.
Variations in digestive enzymes and hormones during the larval development of gilthead seabream (Sparus aurata) fed on live prey (Artemia nauplii) enriched with free methionine were investigated for 16 days (from day 24 to day 40). Prior to initiation of the
experiment, newly hatched larvae were transferred from incubators to fiber glass tanks (300 l) with black walls and fed with
same diets until day 24. Each experiment was performed in triplicate. In the experimental group, the content of the free methionine
in the Artemia nauplii was increased by adding a 5.3 mM free methionine solution to the culture water during a 16-h enrichment period. The larvae of both the control and enriched-methionine
groups were sampled four times, with 4-day intervals between samplings, during a 16-day period. The larvae in the control
group had a significantly lower growth than those of the methionine group at the end of the study (P < 0.05). The highest trypsin activity and leucine aminopeptidase N/leucine–alanine peptidase ratios were observed in the
control group. A significant difference between bombesin activities in the treatment groups was not found at 5th minute after
the initiation of feeding (P > 0.05), but they were significant at 15th minute post-initiation of feeding (P < 0.05). A significant difference between the cholecystokinin levels of the treatment groups was found (P < 0.05). 相似文献
73.
Tom M Bargar Jacob Secor Lowell D Markley Brian A Shaw Jon A Erickson 《Pest management science》1999,55(11):1059-1069
This report describes the development of a Comparative Molecular Field Analysis (CoMFA) model from a set of obtusifoliol 14α-methyl demethylase (DM) inhibitors to aid in the design of herbicides targeting sterol biosynthesis. CoMFA is a three-dimensional (3-D) quantitative structure–activity relationship (QSAR) method that is useful in the probing of receptor binding sites when experimental structure data are unavailable. Conformational analysis and SAR of some rigid and active analogs were used to build the initial model using the active analog hypothesis. The model was subsequently used to design compounds that retain the active site shape requirements, but incorporate physical properties that favor soil-applied herbicidal action. In addition, a second-generation CoMFA model incorporating the newly designed inhibitors was developed and represents the current understanding of the DM binding site. This model was derived from a pharmacophore developed from two methods, the active analog approach as well as from the Catalyst program. The fact that two independent methods produced a similar pharmacophore strengthens the validity of the model. © 1999 Society of Chemical Industry 相似文献
74.
目的 合成6个苯甲酰胺类磷酸二酯酶-4抑制剂。方法 以异香兰素为原料,经过成醚反应、氧化反应、Schotten-Baumann反应等制备目标化合物。结果 成功制备了6个目标化合物:Benzoic acid,4-[[3-(isobutoxy)-4-methoxybenzoyl]amino](T1),Benzamide,4-[[3-(isobutoxy)-4-methoxybenzoyl]amino](T2),Benzamide,4-(isobutoxy)-3-methoxy-N-[4-[[(1-methylethyl)amino]sulfonyl]phenyl](T3),Benzoicacid,4-[[3-(cyclopentyloxy)-4-methoxybenzoyl]amino](T4),Benzamide,4-[[3-(cyclopentyloxy)-4-methoxybenzoyl]amino](T5),Benzamide,4-(cyclopentyloxy)-3-methoxy-N-[4-[[(1-methylethyl)amino]sulfonyl]phenyl](T6),收率分别为66.7%、65.7%、18.4%、46.7%、76.8%和12.4%,其中化合物T1、T2、T3、T5、T6为新化合物。结论 所得产物的化学结构经1H-NMR、MS谱确证,与目标化合物相符。 相似文献
75.
比较研究不同浓度乙烯抑制剂AOA(aminooxyacetic acid)、AIB(α-amino-isobutyric acid)、AVG (Aminoethoxyvinyl glycine hydrochloride)、ATA(3-amino-1,2,4-triazole)、1-MCP(1-methylcyclopropene)、精胺、亚精胺对采后杧果(Mangifera indica L.)乙烯释放的抑制效果,以期筛出最佳乙烯抑制剂并探究其对杧果的贮藏保鲜效果。结果表明:不同浓度乙烯抑制剂处理杧果,均可降低杧果果实乙烯释放量,其中1 mmol/L精胺、10 mmol/L亚精胺和50 mmol/L ATA可有效延缓硬度、可滴定酸(Titratable acid, TA)下降,维持较高VC(Vitamin C)和TSS(Total soluble solids)含量。综合分析,精胺、亚精胺、ATA处理可有效延缓采后杧果果实软化,为采后杧果贮藏保鲜提供技术支持。 相似文献
76.
四种SBIs类杀菌剂对不同发育阶段小麦赤霉病菌的毒力及其作用方式 总被引:3,自引:1,他引:2
为明确不同发育阶段小麦赤霉病菌对甾醇生物合成抑制剂类(SBIs)杀菌剂的敏感性差异及杀菌剂的作用方式,对4种SBIs类杀菌剂进行了室内毒力测定及田间药效试验。室内毒力测定结果表明:不同发育阶段小麦赤霉病菌对4种SBIs类药剂的敏感性存在较大差异,其敏感性由高到低依次为菌丝生长阶段和产孢阶段芽管伸长阶段孢子萌发阶段;其中,戊唑醇对菌丝生长、产孢、芽管伸长和孢子萌发阶段的EC50值分别为0.686、0.191、3.532和3.825μg/m L,咪鲜胺分别为0.063、0.305、0.827和36.581μg/m L,苯醚甲环唑分别为0.873、3.659、5.687和79.465μg/m L,烯唑醇分别为1.961、33.658、41.881和54.986μg/m L。显微观察4种SBIs类杀菌剂对小麦赤霉病菌菌丝生长和孢子萌发形态的影响,发现杀菌剂处理后菌丝末端分枝增多,新生菌丝伸长受阻,菌丝体局部膨大,有时可见菌丝及芽管的消解。田间试验结果表明:用量为有效成分100 g/hm2的戊唑醇、200 g/hm2的咪鲜胺和200 g/hm2的苯醚甲环唑对小麦赤霉病的保护效果为77.15%~79.26%,治疗效果为70.64%~72.77%,于发病前后使用均可有效防治该病害。 相似文献
77.
Alessandro Busetti George Shaw Julianne Megaw Sean P. Gorman Christine A. Maggs Brendan F. Gilmore 《Marine drugs》2015,13(1):1-28
Bacterial epiphytes isolated from marine eukaryotes were screened for the production of quorum sensing inhibitory compounds (QSIs). Marine isolate KS8, identified as a Pseudoalteromonas sp., was found to display strong quorum sensing inhibitory (QSI) activity against acyl homoserine lactone (AHL)-based reporter strains Chromobacterium violaceum ATCC 12472 and CV026. KS8 supernatant significantly reduced biofilm biomass during biofilm formation (−63%) and in pre-established, mature P. aeruginosa PAO1 biofilms (−33%). KS8 supernatant also caused a 0.97-log reduction (−89%) and a 2-log reduction (−99%) in PAO1 biofilm viable counts in the biofilm formation assay and the biofilm eradication assay respectively. The crude organic extract of KS8 had a minimum inhibitory concentration (MIC) of 2 mg/mL against PAO1 but no minimum bactericidal concentration (MBC) was observed over the concentration range tested (MBC > 16 mg/mL). Sub-MIC concentrations (1 mg/mL) of KS8 crude organic extract significantly reduced the quorum sensing (QS)-dependent production of both pyoverdin and pyocyanin in P. aeruginosa PAO1 without affecting growth. A combinatorial approach using tobramycin and the crude organic extract at 1 mg/mL against planktonic P. aeruginosa PAO1 was found to increase the efficacy of tobramycin ten-fold, decreasing the MIC from 0.75 to 0.075 µg/mL. These data support the validity of approaches combining conventional antibiotic therapy with non-antibiotic compounds to improve the efficacy of current treatments. 相似文献
78.
79.
光合作用抑制性除草剂的研究 总被引:1,自引:0,他引:1
文章阐述了除草剂中的几类光合作用抑制剂及其应用特点,指出了色素合成抑制剂应该是未来除草剂发展的方向。 相似文献
80.