Does rainbow trout justify its high rank among alien invasive species? Insights from a nationwide survey in Greece

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光滑鳖甲抗冻蛋白基因(AP-AFP060116)表达规律的研究

研究以克隆的光滑鳖甲抗冻蛋白基因(AP-AFP060116,Apafp)为目的基因,采用半定量RT-PCR技术,首次探讨了该基因在不同低温处理下的表达规律.半定量RT-PCR实验结果表明光滑鳖甲成虫经历长时间冷驯化后,体内Apafp基因的表达水平增高.计算半定量RT-PCR产物电泳图像的灰度值,结果显示在低温处理的第5 d,昆虫体内Apafp基因的表达水平与对照组相比差异显著(P＜0.05).快速冷驯化实验结果显示,处理组与对照组昆虫体内Apafp基因的表达水平没有明显的差异.同时使用Southern blot技术证明光滑鳖甲抗冻蛋白基因家族的存在.     

The application of biochar on soil acidity and other physico‐chemical properties of soils in southern Ethiopia

The aim of this research was to investigate the effect of biochar amendment on soil acidity and other physico‐chemical properties of soil in Southern Ethiopia using a field experiment of three treatments: (1) biochar made of corn cobs, (2) biochar made of chopped Lantana camara stem, and (3) biochar made of Eucalyptus globulus feedstock and a control, in which neither of the biochar was used. Each treatment had three levels of 6, 12 and 18 t ha⁻¹. The experiment was setup with RCBD in a factorial arrangement with three replications. In this regard, a total of 36 plots (each 2 × 2 m size) were applied with three replications to the depth of 0–15cm. From these 36 plots, composite soil samples were collected to the depth of 0–30 cm and analyzed for bulk density, total porosity, pH, soil organic carbon, total nitrogen, available phosphorus, potassium, and exchangeable acidity using standard procedures before and after biochar application. Two‐way ANOVA was also used to analyze the impact of the biochars on soil acidity and other properties. For the treatments that had significant effects, a mean separation was made using Least Significance Difference (LSD) test. The results showed the application of biochar significantly reduced, soil bulk density and exchangeable acidity when compared with a control (p < 0.05). Moreover, the total soil porosity, soil pH, total nitrogen, soil organic carbon, available phosphorus, and potassium were significantly increased in the soil. From among applied biochar treatments, Lantana camara applied at the level of 18 t ha⁻¹ had a higher impact in changing soil physico‐chemical properties. In general, the study suggests that the soil acidity can be reduced by applying biochar as it can amend other soil physico‐chemical properties.     

黑杨派南方型无性系高生长与气候条件的斜率关联度分析

用灰色系统理论的斜率关联度分析法，求得影响黑杨派南方型无性系高生长的主要气候因子依次为：降水量、极低温、极高温、蒸发量、日照时数、积温、年均温（或相至湿度）、相以湿度（或年均温）、无霜期。     

三大区域村庄类型分布的刻画：历史移民解读

中国农村存在巨大的区域差异,从社会结构上看,可以分为华南宗族团结型地区、中部原子化分散型地区和华北小亲族分裂型地区三大区域.通过数据挖掘和分析,发现我国出生性别比的分布极具规律性,显示出区域上的差异.结合中国南方方言形成及南方宗族型文化发育的共同历史移民根源,笔者将南方方言分布图与出生性别比地图及三大区域划分进行比较研究,具体分析了三者间高度关联性的共同历史移民根源.     

论析欧洲的环境政策

当今世界，欧洲环境治理绩效突出。究其原因，环境政策发挥了指导性作用。而这对于环境形势目最严峻的中国具有十分重要的借鉴意义。文中对欧洲环境政策发展历程、原则及挑战进行了细致分析，以供相关研究机构和学者参考。     

南方春大豆不同播季主要农艺性状的变化及其遗传研究

本文以１２个南方春大豆早熟品种（系）和１２个中熟品种（系）为材料，进行春，秋播试验，研究主要农艺性状的秋播变异率，遗传变异系数，遗传力和遗传进度，结果表明：１，两熟期组秋播变异率平均，除分枝起点为负值以外，其它１１个性状在２．３６％－５２．８８％之间，说明秋播对为结性状均有一定影响；熟期组之间各性状秋播变异率的大小亦存在较大差异，如秋播对早熟组株高，主茎节数，结荚节数，结荚高度，主茎荚数和百粒重的     

抗光肩星天牛苏云金芽胞杆菌Bt886菌株的分离及对毒蛋白编码基因的初步鉴定

从拟谷盗虫尸中分离到一株对鞘翅目害虫光肩星天牛幼虫毒杀作用明显的Bt菌株Bt886。幼虫死亡率高达 6 0 % ,存活者生长明显受到抑制。经过晶体形态特征和杀虫谱分析 ,初步确认该菌株属于cry3类。比较cry3类基因的保守序列 ,利用一对cry3特异引物成功地从Bt886菌株中克隆到了一段基因片段并克隆至pGEM -T载体上。测序分析发现该片段与cry3Aa1基因具有很高的同源性 ,确定来自毒蛋白编码基因。Southern杂交分析表明该基因位于该菌株的质粒上。     

南方经济林经营管理策略

根据南方经济林生产现状,分析了南方经济林生产与管理中存在的问题,结合国内外市场对经济林产品需求,提出了保障南方经济林健康,稳定,持续发展的经营管理策略。     

Higher Education on Urban Forestry in Europe: An Overview

In order to meet the demands of modern, information-based society, higher education in natural resource management needs to be transformed. Enhanced mobility of students and staff, multi- and transdisciplinary approaches, as well as innovative educational approaches are called for. Moreover, the urban component needs to be better incorporated, as exemplified by the emerging field of urban forestry. This paper relates to the developments in higher education within the context of education on urban forests and urban trees in Europe. Presented here are the main findings of a comparative European study led by the Danish Forest and Landscape Research Institute within the framework of the EU-funded COST Action E12 'Urban Forests and Trees'. In order to obtain an overview of the status of higher education on urban forestry in Europe, 180 educational institutions in 28 countries were sent a questionnaire. Results show that urban forestry is an expanding element of European education, as urban elements are becom