不同水肥耦合对西芹收获期土壤硝酸盐累积的影响

采用三因素五水平二次通用旋转组合设计,研究了冀西北高寒半干旱区不同水肥耦合对旱棚西芹收获期0 ～ 40 cm土层中硝酸盐累积的影响.结果表明:①在高中低不同水肥处理组合中,较高肥在较高水下可减少0 ～ 40 cm土层土壤硝酸盐的累积,较高肥在较低水下可使土壤硝酸盐相对累积增多;综合因子效应分析表明:随着单因子水平增加,N、P和水(W)对0 ～ 40 cm土层土壤硝酸盐的相对累积依次表现为:增加、不明显和减少.②三因子其他组合下,0 ～ 40 cm土层土壤硝酸盐的累积效应表现为: 在PW一致下,随N量的增加而增加;在NP一致下,随灌水量增加而减少;在NW一致下,随施P量增加而有不确定性.各处理硝酸盐的累积均随土壤深度的增加而增加,体现了水对土壤硝酸盐淋洗的特点.     

四川紫色土区小流域土壤养分流失初步研究

通过对四川紫色土区小流域降雨的观测、径流养分和泥沙含量的分析,初步探讨了紫色土区养分的流失规律。多数研究认为,土壤养分大部分随泥沙携带而流失,而本文的研究结果却表明了:在研究区域,土壤养分流失的主要途径是径流的流失,而随泥沙携带的潜在土壤养分,由于产沙量较少,其流失总量并不多。     

Modeling soil organic carbon dynamics in Oxisols of Ibirubá (Brazil) with the Century Model

Introduction of conservation practices in degraded agricultural land will generally recuperate soil quality, especially by increasing soil organic matter. This aspect of soil organic C (SOC) dynamics under distinct cropping and management systems can be conveniently analyzed with ecosystem models such as the Century Model. In this study, Century was used to simulate SOC stocks in farm fields of the Ibirubá region of north central Rio Grande do Sul state in Southern Brazil. The region, where soils are predominantly Oxisols, was originally covered with subtropical woodlands and grasslands. SOC dynamics was simulated with a general scenario developed with historical data on soil management and cropping systems beginning with the onset of agriculture in 1900. From 1993 to 2050, two contrasting scenarios based on no-tillage soil management were established: the “status quo” scenario, with crops and agricultural inputs as currently practiced in the region and the “high biomass” scenario with increased frequency of corn in the cropping system, resulting in about 80% higher biomass addition to soils. Century simulations were in close agreement with SOC stocks measured in 2005 in the Oxisols with finer texture surface horizon originally under woodlands. However, simulations in the Oxisols with loamy surface horizon under woodlands and in the grassland soils were not as accurate. SOC stock decreased from 44% to 50% in fields originally under woodland and from 20% to 27% in fields under grasslands with the introduction of intensive annual grain crops with intensive tillage and harrowing operations. The adoption of conservation practices in the 1980s led to a stabilization of SOC stocks followed by a partial recovery of native stocks. Simulations to 2050 indicate that maintaining “status quo” would allow SOC stocks to recover from 81% to 86% of the native stocks under woodland and from 80% to 91% of the native stocks under grasslands. Adoption of a “high biomass” scenario would result in stocks from 75% to 95% of the original stocks under woodlands and from 89% to 102% in the grasslands by 2050. These simulations outcomes underline the importance of cropping system yielding higher biomass to further increase SOC content in these Oxisols. This application of the Century Model could reproduce general trends of SOC loss and recovery in the Oxisols of the Ibirubá region. Additional calibration and validation should be conducted before extensive usage of Century as a support tool for soil carbon sequestration projects in this and other regions can be recommended.     

施硒对暗棕壤硒状况和森林蔬菜硒积累的影响

以培育富硒森林蔬菜为目标,在暗棕壤上进行了荚果蕨、小叶芹、小根蒜、婆婆丁等4种山野菜施硒试验。硒肥种类为亚硒酸钠,施硒量为0.10~10.00mg kg-1,直接施肥范围为0~15cm表土。经过一个完整的生长周期(1a)后测定土壤和植物硒指标。结果表明,典型暗棕壤对外源亚硒酸钠态硒具有较强吸收与保持能力,从低剂量至高剂量施入的硒有90%~70%以上仍保留在原施硒土层内;其中H2O可溶态硒占全硒的6.03%~3.78%,KH2PO4浸提的有效态硒占全硒的19.36%~37.54%,而且土壤全硒、KH2PO4浸提的有效态硒及H2O可溶态硒皆与最初的施硒量呈线性正相关。在土施强化供硒条件下,4种山野菜均能高量富集硒,其含硒量为对照的1.7~158.9倍;不同植物的富硒能力有差异,它们在递增施硒情况下高量富集硒的变化模式也各有特点。总体来看,山野菜含硒量与施硒量之间为非线性正相关关系,但在一定的较低施硒量范围内这种关系则是线性的,线性范围因植物而异;土壤的可溶态硒、KH2PO4浸提的有效硒甚至全硒等指标与山野菜含硒量之间亦均呈明显的非线性正相关,且施硒量较低时其关系亦表现为线性。初步得出,典型暗棕壤控制施硒是在近自然条件下培育富硒森林蔬菜并获取高富硒生物资源的有效途径;不过,强烈搅动的砂砾质暗棕壤却不利于硒肥保持和植物吸收,不适于富硒森林蔬菜栽培。     

RZWQM simulation of long-term crop production, water and nitrogen balances in Northeast Iowa

Agricultural system models are tools to represent and understand major processes and their interactions in agricultural systems. We used the Root Zone Water Quality Model (RZWQM) with 26 years of data from a study near Nashua, IA to evaluate year to year crop yield, water, and N balances. The model was calibrated using data from one 0.4 ha plot and evaluated by comparing simulated values with data from 29 of the 36 plots at the same research site (six were excluded). The dataset contains measured tile flow that varied considerably from plot to plot so we calibrated total tile flow amount by adjusting a lateral hydraulic gradient term for subsurface lateral flow below tiles for each plot. Keeping all other soil and plant parameters constant, RZWQM correctly simulated year to year variations in tile flow (r² = 0.74) and N loading in tile flow (r² = 0.71). Yearly crop yield variation was simulated with less satisfaction (r² = 0.52 for corn and r² = 0.37 for soybean) although the average yields were reasonably simulated. Root mean square errors (RMSE) for simulated soil water storage, water table, and annual tile flow were 3.0, 22.1, and 5.6 cm, respectively. These values were close to the average RMSE for the measured data between replicates (3.0, 22.4, and 5.7 cm, respectively). RMSE values for simulated annual N loading and residual soil N were 16.8 and 47.0 kg N ha⁻¹, respectively, which were much higher than the average RMSE for measurements among replicates (7.8 and 38.8 kg N ha⁻¹, respectively). The high RMSE for N simulation might be caused by high simulation errors in plant N uptake. Simulated corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] yields had high RMSE (1386 and 674 kg ha⁻¹) with coefficient of variations (CV) of 0.19 and 0.25, respectively. Further improvements were needed for better simulating plant N uptake and yield, but overall, results for annual tile flow and annual N loading in tile flow were acceptable.     

Long-term impact of fertilization on activity and composition of bacterial communities and metabolic guilds in agricultural soil

To explore long-term impact of organic and inorganic fertilizers on microbial communities, we targeted both the total bacterial community and the autotrophic ammonia oxidizing bacteria (AOB) in soil from six treatments at an experimental field site established in 1956: cattle manure, sewage sludge, Ca(NO₃)₂, (NH₄)₂SO₄, unfertilized and unfertilized without crops. All plots, except the bare fallows, were cropped with maize. Effects on activity were assessed by measuring the basal respiration and substrate induced respiration (SIR) rates, and the potential activity of the AOB. To determine the bacterial community composition, 16S rRNA genes were used to fingerprint total soil communities by terminal restriction fragment length polymorphism analysis and AOB communities by denaturing gradient gel electrophoresis. The fertilization regimes had clear effects on both activity and composition of the soil communities. Basal respiration and r, which was kinetically derived as the exponentially growing fraction of the SIR-response, correlated well with the soil organic C content (r=0.93 and 0.66, respectively). Soil pH ranged from 3.97 to 6.26 in the treatments and was found to be an important factor influencing all microbial activities. pH correlated negatively with the ratio between basal respiration and SIR (r=0.90), indicating a decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass in the most acid soils with pH 3.97 and 4.68 ((NH₄)₂SO₄ and sewage sludge fertilized plots, respectively). The lowest SIR and ammonia oxidation rates were also found in these treatments. In addition, these treatments exhibited individually different community fingerprints, showing that pH affected the composition of AOB and total bacterial communities. The manure fertilized plots harbored the most diverse AOB community and the pattern was linked to a high potential ammonia oxidation activity. Thus, the AOB community composition appeared to be more strongly linked to the activity than the total bacterial communities were, likely explained by physiological differences in the populations present.     

Introduced slugs and indigenous caterpillars as facilitators of carbon and nutrient mineralisation on a sub-Antarctic island

Indigenous soil macroinvertebrates (moth larvae, weevil larvae, earthworms) are cardinal agents of nutrient release from litter on sub-Antarctic Marion Island (47°S, 38°′E). Their populations are threatened through predation by introduced house mice, which do not prey on an introduced slug Deroceras panormitanum. A microcosm study was carried out to explore whether slugs affect rates of carbon and inorganic nutrient mineralisation from plant litter differently to an indigenous caterpillar (larva of a flightless moth Pringelophaga marioni). Caterpillars stimulated N, Ca, Mg and K mineralisation from plant litter two to five times more than slugs did, whereas the two invertebrate types stimulated C and P mineralisation to the same degree. Consequently, ratios of C:N and N:P released from the litter were different for slugs and caterpillars. Such differences might affect peat nutrient quality and ultimately the peat accumulation-decomposition balance, an important driver of ecological succession. This suggests that slugs cannot simply replace caterpillars without consequences for ecosystem structure and functioning on the island.     

Heterotrophic and autotrophic nitrification in two acid pasture soils

Laboratory incubation experiments, using ¹⁵N-labeling techniques and simple analytical models, were conducted to measure heterotrophic and autotrophic nitrification rates in two acid soils (pH 4.8-5.3; 1/5 in H₂O) with high organic carbon contents (6.2-6.8% in top 5 cm soil). The soils were from pastures located near Maindample and Ruffy in the Northeast Victoria, Australia. Gross rates of N mineralization, nitrification and immobilization were measured. The gross rates of autotrophic nitrification were 0.157 and 0.119 μg N g⁻¹ h⁻¹ and heterotrophic nitrification rates were 0.036 and 0.009 μg N g⁻¹ h⁻¹ for the Maindample and Ruffy soils, respectively. Heterotrophic nitrification accounted for 19% and 7% of the total nitrification in the Maindample and Ruffy soils, respectively. The heterotrophic nitrifiers used organic N compounds and no as the substrate for nitrification.     

An empirical approach to target DNA quantification in environmental samples using real-time polymerase chain reactions

Recent advances in molecular techniques have allowed for the routine examination of nucleic acids in environmental samples. Although current methodologies are very sensitive, accurate target DNA quantification from environmental samples remains challenging. To facilitate high-throughput DNA quantification from environmental samples, we developed a novel DNA quantification method based on a non-linear curve-fitting approach to extract additional information from quantitative PCR amplification curves and used the fitted parameters to develop multiple regression standard equations for target DNA quantification. A 3-parameter sigmoidal function performed superior to a 4-parameter Weibull function for generating the multiple regression standard equations. In a verification experiment, target DNA was quantified in a series of ‘unknown’ samples in three soils using this approach and the results were compared to target DNA values determined using corrected and uncorrected Ct-based (threshold cycle) methods. For each method, the deviations from the expected target DNA content were determined. Results clearly showed that over all DNA concentrations, target DNA content determined by the non-linear curve-fitting method was more accurate and more precise than values predicted by all other methods. Analysis of variance conducted on the predicted DNA contents also revealed fewer statistical artifacts with the non-linear curve fitting method compared to the conventional Ct-based methods. The novel approach described here is accurate, inexpensive, and very amenable for automation and high-throughput applications.     

Changes in chemical and biological properties of a sodic clay subsoil with addition of organic amendments

An experiment was performed to examine the chemical and biological effects on high clay sodic subsoil following the incorporation and incubation with organic amendments. The main treatments consisted of amendments with wheat shoots, lucerne pellets and peat, and these were compared to gypsum addition. Additional treatments were residues of chickpea and canola, chicken manure and sawdust. All materials were finely ground and added to crushed and sieved soil at the rate of 1% by weight. Wheat, canola and chickpea residues and chicken manure resulted in modest reductions in soil sodicity. Carbon and N mineralization were related to the soluble C/total N ratio in the amendment. The initial mineralization of wheat amendment was rapid due to its soluble C content, but then slowed to have the lowest loss, of around one third of added C, of all the plant residues after 174 days. In comparison, lucerne-amended soil increased total N and lost almost half of its C after the 174-day incubation. The canola stubble amendment showed the highest carbon loss, losing 64% of its added C. The addition of gypsum resulted in high soil electrical conductivity which suppressed respiration, compared to the control soil, indicating a detrimental effect on microbial activity due to the high electrolyte concentration in the soil. The peat amendment, with a low-soluble C content, showed a similar respiration rate to the control soil, confirming that a source of soluble C is important for the initiation of rapid biological activity. Soil pH was significantly increased (by 0.6 of a pH unit) with addition of chicken manure, and still remained higher than control soil after 174 days of incubation. Lucerne was the only plant residue to increase soil pH, with the effect being sustained for 56 days. The study demonstrated how some organic amendments can improve chemical fertility and biological activity in high clay sodic subsoil, and at the same time contribute, after 25 weeks incubation, to an increase in carbon content.