Physiological and anatomical changes in two rapeseed (Brassica napus L.) genotypes under drought stress conditions

Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed (Brassica napus L.) varieties. The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes, P287 (drought-tolerant) and T88 (drought-sensitive) under three intensities of drought stress. All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions. At the fourth-leaf stage, the relative water content, chlorophyll content, protein content, malondialdehyde content, and the activities of peroxidase and catalase in P287 were significantly higher than those in T88, particularly under severe drought conditions. After rehydration, all physiological indexes recovered rapidly, especially in P287. In addition, under drought stress, compared with T88, P287 had thicker palisade tissue, thinner spongy tissue, higher ratio of chloroplast length to chloroplast width, higher stomatal density and stomatal closure rate. Overall, the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.     

China's alfalfa market and imports: Development,trends, and potential impacts of the U.S.–China trade dispute and retaliations

This study examines the development and trends of China's alfalfa market and imports, identifies key factors for the rapid increase in China's alfalfa imports, and discusses potential impacts of the U.S.–China trade dispute and retaliations on the alfalfa markets and trade in both nations. China's rapid transition toward larger-scale commercial dairy production, with enhanced feed and cost management as well as quality and safety control, and its limited resources for high-quality alfalfa production are key factors for the dramatic increase in its alfalfa imports, from 19 601 metric tons in 2008 to 1.38 million metric tons (mmt) in 2018. While the United States dominated China's alfalfa imports with an average share of 97.01% from 2007 to 2017, the share dropped to 83.76% in 2018 and 63.28% in January 2019 due to the trade dispute and retaliations started in 2018. China will likely remain a large importer of alfalfa because of both its growing demand and the comparative advantages of imported alfalfa in quality and price, but the imports from the United States will be highly affected by the ongoing trade dispute and negotiations. China is also expected to make more efforts to reduce its dependence on U.S. alfalfa through increased investment in domestic alfalfa production and identification of alternative sources of alfalfa and other hay imports.     

Relay-intercropping soybean with maize maintains soil fertility and increases nitrogen recovery efficiency by reducing nitrogen input

Optimized nitrogen(N)management can increase N-use efficiency in intercropping systems.Legume-nonlegume intercropping systems can reduce N input by exploiting biological N fixation by legumes.Measurement of N utilization can help in dissecting the mechanisms underlying N uptake and utilization in legume-nonlegume intercropping systems.An experiment was performed with three planting patterns:monoculture maize(MM),monoculture soybean(SS),and maize-soybean relay intercropping(IMS),and three N application levels:zero N(NN),reduced N(RN),and conventional N(CN)to investigate crop N uptake and utilization characteristics.N recovery efficiency and 15N recovery rate of crops were higher under RN than under CN,and those under RN were higher under intercropping than under the corresponding monocultures.Compared with MM,IMS showed a lower soil N-dependent rate(SNDR)in 2012.However,the SNDR of MM rapidly declined from 86.8%in 2012 to 49.4%in 2014,whereas that of IMS declined slowly from 75.4%in 2012 to 69.4%in 2014.The interspecific N competition rate(NCRms)was higher under RN than under CN,and increased yearly.Soybean nodule dry weight and nitrogenase activities were respectively 34.2%and 12.5%higher under intercropping than in monoculture at the beginning seed stage.The amount(Ndfa)and ratio(%Ndfa)of soybean N2 fixation were significantly greater under IS than under SS.In conclusion,N fertilizer was more efficiently used under RN than under CN;in particular,the relay intercropping system promoted N fertilizer utilization in comparison with the corresponding monocultures.An intercropping system helps to maintain soil fertility because interspecific N competition promotes biological N fixation by soybean by reducing N input.Thus,a maize-soybean relay intercropping system with reduced N application is sustainable and environmentally friendly.     

核桃新品种‘鲁绵1号’

‘鲁绵1号’核桃是从‘鸡爪绵’核桃实生群体中选育出的高油抗病新品种。坚果圆形,单果质量15.0 ~ 17.3 g,易取整仁,出仁率46.2%;脂肪含量70.5% ~ 72.0%,蛋白质含量18.8% ~ 20.3%。丰产,抗病。在济南市南部山区8月下旬果实成熟,10年生树平均株产4.9 kg,折合3 013.5 kg • hm-2。     

四种短体线虫的形态和分子生物学鉴定

 采用形态学和分子生物学方法对来自荷兰的藏橐吾和铃兰、泰国的高山榕、缅甸的香蕉等种苗(球)分别鉴定出玻利维亚短体线虫Pratylenchus bolivianus、铃兰短体线虫P. convallariae、咖啡短体线虫P. coffeae和斯佩奇短体线虫 P. speijeri。对这4种线虫的形态特征进行较为详细的描述后,认为头环、口针、侧区、生殖系统和尾形等形态特征是种类鉴定的重要依据;进一步利用引物28S-D2A/28S-D3Br扩增测序得到上述4种线虫的28S rRNA基因D2/D3区序列,序列分析发现玻利维亚短体线虫从欧洲到美洲的不同种群之间的遗传距离变异较小,仅为0~0.007;铃兰短体线虫同一种群不同个体之间的遗传变异较大,为0.006~0.029;咖啡短体线虫不同种群之间的平均遗传距离为0.013;斯佩奇短体线虫不同种群之间的平均遗传距离为0.010;咖啡短体线虫P. coffeae和斯佩奇短体线虫P. speijeri亲缘关系很近,二者种间平均遗传距离仅为0.026。本研究再次证明线虫28S rRNA基因D2/D3区基因序列可作为短体线虫种间鉴定的依据。     

利用广亲和基因S5-n的功能标记鉴定特殊配组类型杂交种纯度研究

“粳不育系/广亲和恢复系”配组方式在浙江省得到了越来越多的应用。广亲和基因S5-n的功能标记可快速检测种子纯度。为验证水稻广亲和基因S5-n功能标记鉴定粳不育系/广亲和恢复系配组方式杂种一代的效果,我们分别将长粳1A和6个恢复系获得的F₁代进行大田统计和分子标记鉴定,结果显示,由于广亲和基因S5-n分子标记不能特异性检测出串粉形成的异形株,导致其鉴定结果较大田统计鉴定结果高1.0~2.0百分点。未来还需要进一步优化广亲和基因分子标记技术的特异性,进而提高分子检测的准确性,这将有助于杂交稻的安全生产。     

中美贸易摩擦对广东省输美水产品贸易的影响及对策研究

【目的】通过分析中美贸易摩擦对广东省输美水产品贸易的影响,提出相关对策与建议以缓和中美贸易摩擦带来的负面影响,促进广东省水产品企业转型升级。【方法】结合统计数据及半结构式实地调研情况,运用TC指数方法,分析广东省输美水产品贸易现状,从宏观到微观深入探讨中美贸易摩擦背景下广东省输美水产品市场所受到的影响,并从企业、行业和政府层面提出对策与建议。【结果】2018年中美贸易摩擦发生后,广东省水产品出口贸易面临更大的不确定性,宏观上导致水产品竞争力下降,贸易顺差转为逆差,水产品出口美国受阻,内销比例增大,部分出口美国的水产品转向东盟和澳大利亚市场;微观上对水产品上中下游各环节造成负面影响,非美和国内市场有所拓宽,加工量减少,养殖计划不明确。【结论】广东省水产品企业应兼顾国内外市场,激发消费潜力;带领养殖户转变养殖模式,提高抗风险能力;加大科研力度,从根本上提升核心竞争力。行业协会应发挥带头作用,共同防范风险。建议政府出台相关扶持政策,携手企业以更从容的姿态应对中美贸易摩擦。     

规模化畜禽养殖场粪便中多重耐药菌分离鉴定及其耐药特征

为了解禽畜粪便中多重耐药菌的污染特征,本研究对鸡粪、牛粪、猪粪和有机肥这4种不同样品中多重耐药菌进行了计数分析,并对不同来源的多重耐药菌株进行了分离和纯化,进一步开展了基于16S rDNA序列比对的分子生物学鉴定以确定其种属地位,以及基于药敏试验的耐药性特征分析。结果表明:不同养殖动物粪便中四环素、恩诺沙星、磺胺甲恶唑和泰乐菌素4种抗生素多重耐药菌的绝对数量和相对数量排序均为鸡粪>猪粪>牛粪,粪源有机肥中可培养的多重耐药菌的绝对数量和相对数量在堆肥后均有所下降。通过对耐药菌株鉴定和分类学分析,发现禽畜粪便中多耐药菌的菌门集中分布在Proteobacteria、Firmicutes和Actinobacteria,多重耐药菌的优势菌属为Escherichia、Corynebacterium、Kurthia;而有机肥样品中多重耐药菌的优势菌属为Staphylococcus、Glutamicibacter。菌株的药敏试验表明,3类动物粪污中的多重耐药菌都对红霉素、四环素有着较高的耐药率,均高于80%,而对阿米卡星这种抗生素表现出较好的敏感性,其耐药率低于30%。通过对Ⅰ、Ⅱ类整合子基因盒的扩增,发现PCR产物的大小从0.8 kb到1.8 kb不等,基因盒ad A2、dfr A17、dfr A1及sat2在养殖场粪污中检出率均较高。     

短轮伐期桉树人工林个体林木特征研究

利用广东高要和广西扶绥两地14个2~10年生桉树林分样地共287株样木,研究短轮伐期桉树人工林个体林木特征指标,并比较现有广西良种桉二元带皮和去皮材积公式估算个体林木材积的准确性,分析不同年龄、林分和径阶林木的出材率。结果表明:带皮和去皮林木的胸高形数为0.504±0.004 2和0.529±0.004 4、实验形数为0.410±0.002 4和0.427±0.002 7、形率为0.657±0.003 4和0.690±0.003 6、高径比为1.22±0.017和1.43±0.019。用广西良种桉二元带皮和去皮材积公式估算单株林木带皮和去皮材积与实际材积高度相关(r>0.99),但估算值普遍偏低,平均分别小3.7%和21.0%;尤以用带皮材积公式估算去皮材积比用去皮材积公式估计要准确,平均只小2.3%。如以6年生以上样地和尾径4 cm以上的去皮林木为实际出材,5个相关样地林分平均出材率为(73.3±4.31)%,最小为54.6%,最大为81.5%。