NIH3T3 cell proliferation and cell cycle in the conditions of hypoxia or low nutrition and its response to bFGF

AIM: To investigate NIH3T3 cell proliferation and cell cycle in the condition of hypoxia or low nutrition and its response to bFGF, and to explore the pathophysiological changes of fibroblast under hypoxic or low nutrional conditions.METHODS: The cells were placed in anaerobic workstation where the mixture gas was given to simulate hypoxic environment. Partial oxygen pressure (PO2) of medium was controlled in 27 mmHg, 44 mmHg and 175mmHg. NIH3T3 cells were cultured with low nutritional medium contained new bovine serum (NCS) less than 10% to simulate low nutritional environment. MTT assay was used for observing cell activity and flow cytometry for cell cycle analysis.RESULTS: Under 44 mmHg PO2, no obvious difference was shown between hypoxia group and normal group. Under 27 mmHg PO2, the proliferation activity of NIH3T3 cells was significantly lower than that in normal group (P<0.01), as well as the cell numbers in G0-G1 phase increased (P<0.05), S phase decreased (P<0.01). bFGF had no effect on cell proliferation. In 0.5% NCS medium, the NIH3T3 cell proliferation speed decreased (P<0.01) and cell cycle was arrested at G0-G1 (P<0.01). The proliferation speed was improved by bFGF (P<0.01).CONCLUSION: In lower PO2 or lower nutrinal condition, fibroblast proliferation activity is inhibited by cell cycle arrest in G0/G1 phase. However the decreasing proliferation in low nutritional medium could be improved by external bFGF.     

基于SPA-SSA-BP的小麦秸秆含水率检测模型

为提高基于电容法的小麦秸秆含水率检测模型的检测精度,扩大含水率检测范围,提高模型适应性,本文以小麦秸秆为研究对象,使用LCR数字电桥,测量含水率为10.43％ ～25.89％的秸秆在频率0.05 ～ 100 kHz、容积密度90.03 ～179.42 kg/m3和温度25 ～40℃内的电容,利用连续投影法(Succes...     

基于高光谱技术的茶鲜叶含水率检测与分析

茶鲜叶含水率是茶叶加工业中衡量茶叶品质的一个重要指标。为了实现茶叶加工过程中茶鲜叶含水率的快速检测,本文提出了一种应用高光谱技术分析茶鲜叶含水率的无损检测方法。通过对茶鲜叶高光谱图像感兴趣区域光谱数据的提取,利用4种不同的算法对原始数据进行预处理,采用逐步回归分析法对预处理后的数据提取特征波长,并采用多元线性回归法、偏最小二乘回归建立特征波长和茶鲜叶含水率定量分析模型。研究结果表明,经过卷积平滑处理后的正交信号校正的预处理结合逐步回归分析法所建立的偏最小二乘回归茶鲜叶含水率预测效果最佳,模型校正集、交叉验证集和预测集的相关系数分别为0.8977、0.8342和0.7749,最小均方根误差分别为0.0091、0.0311和0.0371。由此可见,高光谱技术能有效的实现茶鲜叶含水率的检测,这为茶叶加工业中衡量茶叶品质提供了新的检测方法。     

水肥调控对二月兰和后茬花生养分累积及土壤肥力的影响

摘 要：田间试验条件下，研究不同水肥处理对二月兰生长，及其翻压后后茬花生产量和养分累积的变化。结果表明，灌溉和施肥可显著促进二月兰生长。在绿肥季，不论施肥与否，灌溉处理均可显著提高二月兰的生物量和N、P、K养分含量， NPW（绿肥季施氮磷肥和灌溉）和CKW处理（绿肥季不施肥，只进行灌溉处理）的二月兰生物量和N、P、K养分含量分别比相应的未灌溉处理提高了66.47%和63.97%、76.95%和32.36%、88.31%和9.80%、21.71%和15.56%。二月兰翻压的养分还田量为91.04～260.23 kg/hm2，约占花生季化肥总养分的27.59%～78.86%。与冬闲处理（CF）相比，不同施肥和灌溉处理的绿肥翻压均促进了花生产量和养分累积，及土壤养分含量的提高，其中以EN处理的提升效果最明显。周年等养分条件下，花生季35.00%氮和/或42.86%磷肥料前移至绿肥季，可明显促进绿肥养分还田量的增加，后茬花生产量不同程度增加（增幅22.82%～41.18%）。综上，在适量灌溉和施肥条件下，二月兰生物量明显增加，进而促进后茬花生产量增加及养分累积。研究结果可为我国绿肥农田应用及化肥减施提供数据支撑和实践依据。     

30个甜高粱品种茎秆糖产量与干物质生产特性相关研究

甜高粱是一种主要的生物质能源作物,加强甜高粱品种改良与高产栽培对保障我国能源供应及能源安全具有重要意义。在充分发挥个体生产潜力条件下(大田种植密度15 000株/hm2),研究了国内外30个甜高粱品种茎秆糖含量与抽穗前和抽穗后干物质积累特性。结果表明,对成熟期糖产量起直接贡献作用的性状由大到小排序为:抽穗期干物重、抽穗期含糖量、抽穗后干物重所占比例和抽穗后含糖量。其中,抽穗期干物重对糖产量的直接通径系数与其简单相关系数接近,而抽穗期含糖量与此时期干物重呈极显著正相关,最终与糖产量密切相关。抽穗后含糖量由于与抽穗期干物重显著负相关,使其对成熟期糖产量的负间接效应大于自身正直接效应,表现与糖产量弱负相关;抽穗后干物重所占比例则总间接负效应远大于自身正直接效应,最终与糖产量呈极显著负相关。因此,提高茎秆含糖量基础上增加抽穗前干物质积累量,是甜高粱高糖产量品种选育的重要目标。     

Identification and verification of quantitative trait loci for eating and cooking quality of rice (Oryza sativa)

This study was conducted to identify new quantitative trait loci (QTLs) that have stable effects for eating and cooking quality (ECQ) of rice. Three recombinant inbred line populations of indica rice were each planted in two years. Three traits for ECQ, amylose content (AC), gel consistency (GC) and alkali spreading value (ASV), were measured for QTL analysis. A total of 13 QTLs were detected, including four for AC, six for ASV and three for GC. Two QTLs, qGC4 in the interval RM16252–RM335 on the short arm of chromosome 4 and qGC6.2 in the Alk region, were validated in a population derived from a residual heterozygote that was homozygous at the major locus Wx. In the absence of segregation at the Wx locus, qGC4 and qGC6.2 had additive effects of 2.46 and 8.18 mm, respectively, offering a potential for improving GC property of rice varieties. Comparison between qGC4 and previous results suggests that qGC4 is likely a new QTL for GC, providing a candidate for gene cloning and functional characterization.     

养分胁迫对油菜碳酸酐酶活性的影响

对养分胁迫下油菜中碳酸酐酶活性和叶绿素含量的变化进行了研究。结果表明:缺乏氮磷钾素时,油菜的碳酸酐酶活性和叶绿素含量均有所下降;氮磷钾缺乏对油菜的生长也有不同程度的影响。     

试论微型学习之于传统学习的优势

微型学习是以微型内容为特点,微型媒体为支撑的非正式学习模式。它适应了当代生活语境下人们对知识获取方式创新的要求,倡导了学习者尊重自身生命体验的情感回归,体现了人作为受教育者的社会性发展的重要路径,同时也为培养学习者综合能力提供了重要平台。     

日粮添加碳酸氢钠对蛋鸡肠道菌群多样性的影响

900羽21周龄海兰褐蛋鸡随机分成5组,每组6个重复,每个重复30羽。分别在基础日粮中添加0、0．1％、0．5％、2．5％、5％NaHCO3,持续10周。结果显示,与对照组相比,0．1％组蛋鸡盲肠乳酸菌数量增加,大肠杆菌数量降低,菌群多样性指数提高（P〉0．05）。0．5％组蛋鸡粪便pH值显著提高（P〈0．05）,乳酸菌数量增加,菌群多样性指数提高（P〉0．05）。2．5％、5％组蛋鸡粪便pH值、水分含量极显著高于其他组（P〈0．01）。同时,2．5％、5％组盲肠乳酸杆菌数量降低（P〉0．05或P〈0.05）,大肠杆菌数量增高（P〉0．05或P〈0．05）,5％组菌群多样性指数亦显著降低前3组（P〈0．05）。结果表明,日粮添加NaHCO3超过2．5％,蛋鸡腹泻严重,肠道菌群平衡严重破坏。而当NaHCO3的添加量低于0．5％时,肠道有益菌数量和菌群多样性指数呈增加趋势,蛋鸡生产性能维持正常。因此,本试验提示蛋鸡生产中饲料添加剂NaHCO3最高耐受量为0．5％。     

基于Matlab的自动变速器电控单元快速控制原型设计

利用图形建模方法和快速控制原型技术对机械式自动变速器(AMT)电控单元进行开发设计。首先,用matlab的simulink模块建立适合于快速控制原型的AMT电控单元的仿真模型,其次,通过编写S函数建立S函数模块实现AMT仿真模型与实物之间的IO接口,最后,利用matlab自身集成的Real-Time Workshop和Real-Time Windows Target工具实现对AMT电控单元快速控制原型开发。