古兰蕙不是今兰花——也谈舒迎澜先生的《兰花与兰草之辨》

仔细考证大量古文后,针对舒迎澜先生《兰花与兰草之辩》一文提及的古兰蕙、兰草及今日所说之兰花的概念理解,提出自己的观点：兰花与兰草为同物异名,无需再辩证;舒迎澜先生文中将古兰蕙误认为是今兰花有失妥当。文章对古今兰蕙争论的历史由来等进行考证,结论是：古兰蕙是一类高价值芳香药用植物,并非今日所说之兰花。     

蝴蝶兰茎尖培养脱病毒技术初步研究

对培养基的无机盐浓度、钾浓度比例、氮素型态、植物生长调节剂进行了试验优化,研究了培养基和外植体取材对蝴蝶兰茎尖生长点培养脱病毒的影响。结果表明：经MS培养基改良的KN培养基,其1/5大量元素的无机盐浓度适合蝴蝶兰茎尖生长点的培养。一定浓度范围内,提高氮源中硝酸态氮的比例,或提高培养基中钾的浓度比例,均有助于蝴蝶兰茎尖生长点培养的存活。植物生长调节剂NAA0.5mg.L-1＋6-BA3.5mg.L-1的组合适合蝴蝶兰茎尖生长点培养的存活和类原球茎（Protocorm-Like Body,PLBs）的诱导。茎尖取材大利于培养存活,但是不利于脱病毒效果。齿舌兰轮斑病毒（简称ORSV）的茎尖生长点脱病毒难度较建兰花叶病毒（简称CymMV）的脱病毒难度大。蝴蝶兰茎尖培养脱病毒取材外植体须小于0.4mm,附带叶原基不能多于1个。     

Long-term influence of mowing on population dynamics in the rare orchid Dactylorhiza lapponica: The importance of recruitment and seed production

Many orchids are currently red-listed due to changes in land use, and their future persistence will depend on management. Traditional land use like mowing is believed to favour orchids through increased survival and reproduction of established individuals, but the lack of data connecting their complex life cycle presently limits our ability to evaluate effects of management. Here we used data from 16 years of demographic monitoring to study how mowing affects population dynamics in two populations of the rare orchid Dactylorhiza lapponica. Both populations were characterized by long-lived individuals, low adult mortality and high seedling mortality. The traditional regime of mowing every second year strongly increased recruitment and reduced seed production in both populations, but had moderate effect on adult survival, growth rate and flowering probability. Population growth rate was positive for all population × treatment combinations. Traditional mowing significantly increased growth rate in both populations, and LTRE-analyses revealed that this primarily was a result of increased recruitment. The results indicate that demographic rates commonly associated with orchid persistence may be insensitive to traditional management, and underscores the importance of seed production and recruitment to maintain population growth in this long-lived species. The combination of low establishment success and no seed bank makes D. lapponica dependent on high seed input. Our results suggest that D. lapponica would benefit most from traditional mowing performed after seed dispersal in the study areas, but also suggest a high probability of future survival in the absence of mowing.     

兰花产业化的探讨

兰花是我国名花，分析了我国目前兰花产业化面临的问题，提出了推进兰花产业化的几条途径，并强调了对野生兰花种质的保护与开发利用。     

杭州花圃兰苑植物景观营造探究

通过查阅资料和实地调查,对杭州花圃兰苑植物景观的营造进行深入研究,总结了兰苑植物景观的特色并提出了其存在的不足,以期为传统文化与现代手法相融合的植物景观营造提供一定的理论依据。     

韩国果园菌根真菌的遗传特性

运用现代分子技术对分离于韩国果园中的共生真菌进行了鉴定,经显微观察及PCR-RAPD分析可将收集菌株分为不同组群。不同植物、不同真菌的共生反应类型不同,所有采集的真菌分离株均能刺激果树根的生长,有时甚至能刺激兰属温带果树茎干的生长。5种真菌的18s rDNA序列经BLAST软件分析,证明它们分属于3个组,其中有2个种;葡萄丝粒菌及内生丝核菌可以确定,基于18 s rDNA树状分枝图与植物反应类型的不同相一致,但与PCR-RAPD多态性分析结果不同,韩国当地春兰分离株与严重的土传病害丝核菌及镰刀菌不同,这种不同可通过不同引物的PCR-RAPD技术进行鉴别。     

兰花圆斑病的研究

１９９５年，在深圳梧桐出兰圃发现一种新的兰花圆斑病，该病害在深圳对兰花造成很大危害。２月中旬开始发病，４－５月为发病盛期，高温，阴雨，高湿的天气条件有利于该病的发生。对该病菌的生物学特性及１２种药剂地其孢了了萌发的抑制效应也做了测定。     

兰花组织培养的褐化现象及控制研究进展

综述了兰花组织培养过程中外植体褐化发生的机理及其影响因素,就其解决途径进行了概述,对解决兰花组织培养中的褐化问题具有一定的理论和实践意义。     

Phloroglucinol enhances recovery and survival of cryopreserved Dendrobium nobile protocorms

The aim of the present study was to evaluate the effect of phloroglucinol in the recovery and survival of cryopreserved Dendrobium nobile protocorms. The exposure of protocorms to 2 M glycerol osmoprotective solution for 20 min followed by 10 min in PVS2 vitrification solution with 1% phloroglucinol resulted in the highest protocorm recovery and survival (68%). A positive effect of phloroglucinol was observed when combined with glycerol and PVS2. Phloroglucinol added at 1% provided an increase of over 100% in protocorm recovery and survival as compared to the same treatment without phloroglucinol. However, when sucrose was added to treatments, a negative effect was observed with a reduction in survival by 90%. Protocorms that survived cryopreservation were successfully regenerated into plants and acclimatized with 100% survival in greenhouse. Survival of cryopreserved D. nobile protocorms was a determining factor for seedling survival and growth into normal and fully functional plants. This study demonstrated an efficient procedure for cryopreservation and subsequent recovery and survival of cryopreserved D. nobile protocorms using phloroglucinol as a cryoprotectant additive.     

Night interruption promotes vegetative growth and flowering of Cymbidium

The effects of night interruption (NI) were examined on the vegetative growth and flowering of Cymbidium ‘Red Fire’ and ‘Yokihi’. Plants were grown under 9/15 h ambient light/dark (control), 9 h ambient light plus night interruption (22:00–02:00 h) with low light intensity at 3–7 μmol m⁻² s⁻¹ (LNI) and 9 h ambient light plus NI with high light intensity at 120 μmol m⁻² s⁻¹ (HNI) conditions. The number of leaves, leaf length, number of pseudobulbs and pseudobulb diameter increased in both LNI and HNI compared to controls for both cultivars. While none of the control plants flowered within 2 years, 100% of the ‘Yokihi’ and 80% of the ‘Red Fire’ plants grown under HNI condition flowered. In the LNI group, 60% of the plants flowered in both cultivars. Plants in the HNI group showed a decreased time to visible inflorescence and flowering than those in the LNI group. The number of inflorescences and florets were greater in the plants grown under HNI than those in the LNI group. The tallest plants at flowering were in the HNI group in both cultivars. NI with low light intensity can be used effectively to promote flower induction with increased growth rate during the juvenile stage in Cymbidium. To obtain high quality plants, however, NI with high light intensity strategies should be considered.