转Bt基因抗虫棉施钾效应研究初报

试验于2002—2003年分别在安徽合肥及宿州就安徽省推广的主要抗虫棉品种,国抗1号、中棉所29及32B的施钾效应进行了研究。结果表明:(1)在每公顷施用尿素450kg、磷肥600kg条件下,Bt抗虫棉增施钾肥能明显提高其产量,皮棉产量增加2.3%￣18.9%,品质也有所改善,以施用氯化钾375kg/hm2为宜。增产的主要原因是施钾提高了单株果枝数、单株结铃数、单铃重和衣分,优质铃率也明显提高;(2)在施钾量相同条件下,不同施钾方法对产量也有显著影响,以基施与追施比6:4的方式肥效更佳;(3)中棉所29施钾处理较对照增产12.7%,32B较对照增产11.2%,产量差异均极显著;中棉所29较32B增产6.7%,且差异显著,其主要生理原因是单株干物质积累多,尤其是中后期的干物质积累速率明显高于32B,显示出杂交抗虫棉具有明显的杂种优势。     

氮钾肥用量对水稻新品种混制一号生长及产量的影响

采用大田随机区组设计,研究了氮、钾肥用量对"混制一号"生长及产量的影响。结果表明:氮钾肥用量对"混制一号"分蘖发生、株高、第一伸长节间的长度及重量、产量因素及产量均有较大的影响。其中以A2B3处理(375kg/hm2尿素加375kg/hm2氯化钾)的产量最高,达9154.6kg/hm2,较对照A1B1(225kg/hm2尿素加225kg/hm2氯化钾)增产900.5kg/hm2,增产幅度为10.91%。     

华北平原农田生态系统钾平衡研究

华北平原大部分农田土壤发育在黄河冲积物母质上,矿物钾含量较高,在当前中低产农田中,尤其在有一定秸秆还田的条件下,施用化肥钾增产效果不显著,过多施钾会出现“奢侈性消耗”。在高产条件下,适当施钾对增产仍有一定作用。农田生态系统钾平衡研究对作物生产有重要意义。本文通过3年试验探讨了华北平原农田钾平衡和供钾能力。     

Effect of potassium phosphate fertilization on production and emission of methane and its C-stable isotope composition in rice microcosms

Rice fields are an important source for atmospheric CH₄, but the effects of fertilization are not well known. We studied the turnover of CH₄ in rice soil microcosms without and with addition of potassium phosphate. Height and tiller number of rice plants were higher in the fertilized than in the unfertilized microcosms. Emission rates of CH₄ were also higher, but porewater concentrations of CH₄ were lower. The δ¹³C values of the emitted CH₄ and of the CH₄ in the porewater were both 2-6% higher in the fertilized microcosms than in the control. Potassium phosphate did not affect rate and isotopic signature of CH₄ production in anoxic soil slurries. On the other hand, roots retrieved from fertilized microcosms at the end of incubation exhibited slightly higher CH₄ production rates and slightly higher CH₄-δ¹³C values compared to roots from unfertilized plants. Addition of potassium phosphate to excised rice roots generally inhibited CH₄ production and resulted in increasingly lower δ¹³C values of the produced CH₄. Fractionation of ¹³C during plant ventilation (i.e. δ¹³C in pore water CH₄ versus CH₄ emitted) was larger in the fertilized microcosms than in the control. Besides plant ventilation, this difference may also have been caused by CH₄ oxidation in the rhizosphere. However, calculation from the isotopic data showed that less than 27% of the produced CH₄ was oxidized. Collectively, our results indicate that potassium phosphate fertilization stimulated CH₄ emission by enhancing root methanogenesis, plant ventilation and/or CH₄ oxidation, resulting in residence times of CH₄ in the porewater in the order of hours.     

Effects of nitrification inhibitors on denitrification of nitrate in soil

Summary The influence of 28 nitrification inhibitors on denitrification of nitrate in soil was studied by determining the effects of different amounts of each inhibitor on the amounts of nitrate lost and the amounts of nitrite, N₂O and N₂ produced when soil samples were incubated anaerobically after treatment with nitrate or with nitrate and mannitol. The inhibitors used included nitrapyrin (N-Serve), etridiazole (Dwell), potassium azide, 2-amino-4-chloro-6-methylpyrimidine (AM), sulfathiazole (ST), 4-amino-1,2,4-triazole(ATC),2,4-diamino-6-trichloromethyl-s-triazine (CL-1580), potassium ethylxanthate, guanylthiourea (ASU), 4-nitrobenzotrichloride, 4-mesylbenzotrichloride, sodium thiocarbonate (STC), phenylmercuric acetate (PMA), and dicyandiamide (DCD).Only one of the nitrification inhibitors studied (potassium azide) retarded denitrification when applied at the rate of 10 g g^–1 soil, and only two (potassium azide and 2,4-diamino-6-trichloromethyl-s-triazine) inhibited denitrification when applied at the rate of 50 g g^–1 soil. The other inhibitors either had no appreciable effect on denitrification, or enhanced denitrification, when applied at the rate of 10 or 50 g g^–1 soil, enhancement being most marked with 3-mercapto-1,2,4-triazole. Seven of the inhibitors (potassium azide, sulfathiazole, potassium ethylxanthate, sodium isopropylxanthate, 4-nitrobenzotrichloride, sodium thiocarbonate, and phenylmercuric acetate) retarded denitrification when applied at the rate of 50 g g^–1 soil to soil that had been amended with mannitol to promote microbial activity.Reports that nitrapyrin (N-Serve) and etridiazole (Dwell) inhibit denitrification when applied at rates as low as 0.5 g g^–1 soil could not be confirmed. No inhibition of denitrification was observed when these compounds were applied at the rate of 10 g g^–1 soil, and enhancement of denitrification was observed when they were applied at the rate of 50 or 100 g g^–1 soil.     

钾、尿素与有机物料或双氰胺配施对菠菜体内硝酸盐、亚硝酸盐含量影响的研究

采用盆栽试验探讨了钾、尿素与有机物料或双氰胺配施对菠菜体内硝酸盐、亚硝酸盐累积量及与其相关的硝酸还原酶(NR)活性的影响。结果表明,菠菜体内硝酸盐含量的顺序为:尿素>尿素+鸭粪>尿素+猪粪>尿素+双氰胺; 亚硝酸含量顺序为:尿素>尿素与鸭粪配施>尿素与双氰胺配施>尿素与猪粪配施。有机物料的加入均可明显地增加植株体内NR的活性,在菠菜体内NR活性以:尿素+鸭粪>尿素+猪粪>尿素+双氰胺>尿素处理。在相同的氮肥处理条件下,配施适量的钾肥也能显著地降低菠菜体内硝酸盐、亚硝酸盐的累积和增加NR的活性。随着追肥时间的延长,菠菜体内的硝酸盐、亚硝酸盐含量也随之降低,特别是NR的活性在追肥后的第12天到18天之间有显著的降低过程。     

土壤非交换性钾释放动力学特征及其生物

采用生物耗竭法和0.2mol L-1的NaTPB化学浸提法,利用不同动力学方程对我国8种主要土壤非交换性钾的释钾特性及其生物有效性进行定量评估。结果表明,供试土壤非交换性钾释放分为快速释放和稳定释放两个阶段,一级动力学方程、Elovich 方程、双常数曲线方程和抛物线扩散方程均能描述土壤非交换性钾的释放过程。根据双常数方程中的参数（a）所表征的土壤非交换性钾释放速率常数,供试8种土壤的释钾能力按取土地区存在着自西向东、自北向南渐减的趋势。盆栽试验中,在累积释钾量高且释放速率快的土壤上,黑麦草累积生物量、累积吸钾量和相对含钾量较高,表现出强的供钾能力;与此相反,累积释放量低且释放速率慢的土壤上,黑麦草各项指标偏低,供钾能力较弱。以黑麦草钾素丰缺临界值34.00 mg kg-1 DW-1和土壤非交换性钾释放速率396.32 mg kg-1 h-1作为评价指标,供试8种土壤中,仅取自新疆石河子、陕西常武和黑龙江哈尔滨地区的土壤在短期不施用钾肥的情况下能保证黑麦草丰产且不出现缺钾症状。因此,为了维持土壤钾素平衡和保证作物高产稳产,在我国西北、东北地区耕地土壤中应加强秸秆的循环利用,而在华中和华东地区则需增施钾肥和有机肥。     

自生固氮菌对土壤钾的活化作用

以土壤为钾源,通过液培试验研究了5株自生固氮菌(Azotobacter sp.)对土壤钾的活化作用。结果表明,自生固氮菌能分泌大量的氢离子,大幅度降低培养液p H,使氢离子浓度提高40倍以上。自生固氮菌分泌有机酸的种类与数量因菌株不同而异,这些有机酸包括甲酸、乙酸、草酸、丁二酸、柠檬酸、苹果酸和乳酸等。其中,全部供试菌株均能分泌草酸和苹果酸。在自生固氮菌的培养液中,钾浓度显著高于未接种的培养液,但土壤矿物结构钾则显著降低。由于土壤是培养液钾的唯一来源,说明自生固氮菌可促进土壤矿物钾溶解。相关分析表明,土壤矿物结构钾与自生固氮菌的有机酸分泌总量呈显著负相关(r=-0.845*,n=6),与培养液p H呈显著正相关(r=0.702*,n=6),说明自生固氮菌分泌的有机酸和氢离子可能溶解土壤矿物钾。考虑到自生固氮菌的草酸分泌量最大,络合钙、镁、铁、铝的能力最强,且与有机酸分泌总量呈极显著正相关(r=0.990**,n=6),推测草酸的分泌在活化土壤无效钾的过程中起重要作用。接种自生固氮菌显著降低土壤无效钾,活化能力因菌株不同而异,其原因可能与有机酸分泌的数量和种类有关。     

施钾对扬麦158等小麦品种的养分吸收与生物产量的影响

采用田间试验、室内分析与数理统计相结合的方法研究了不同施钾水平对扬麦158在不同生育期体内的氮（N）、磷（P）、钾（K）含量、吸收量及干物质积累量的影响，还比较了同一施肥水平下，扬麦158等7个品种的养分吸收差异。结果表明，拔节-孕穗阶段是小麦养分吸收和生物产量积累的最快时期。不同生育阶段除含磷量较为稳定外，氮、钾变幅大，因此，在制定施肥方案时，应重视氮、钾的合理配合，以满足小麦“高产、优质、高效”生产的营养需要。     

外界K＋水平对水稻幼苗耐盐性的影响

外界Ｋ~＋水平对水稻幼苗耐盐性的影响晏斌，戴秋杰（江苏省农业科学院，南京２１００１４）ＥｆｆｅｃｔｏｆＥｘｔｅｒｎａｌＫ~＋ＬｅｖｅｌｏｎＳａｌｔＴｏｌｅｒａｎｃｅｏｆＲｉｃｅＳｅｅｄｌｉｎｇｓ￥ＹＡＮＢｉｎ，ＤＡＩＱｉｕｊｉｅ（ＪｉａｎｇｓｕＡｃａｄ...