Influence of leaf litter types on microbial functions and nutrient status of soil: Ecological suitability of forest trees for afforestation in tropical laterite wastelands

The impact of forest tree leaf litters on microbial activity and nutrient status of red laterite soil was tested for the ecological suitability of Cassia siamea, Shorea robusta, Acacia auriculiformes and Dalbergia sissoo, which are typically used for afforestation of wastelands in eastern India. The objectives were to compare seasonal variation in soil enzyme activity in 30-years old afforested sites, and to study nutrient status and microbial biomass and function during short-term in-situ incubation of litter in decomposition pits. In afforested soils, enzyme activities significantly varied between litters and seasons. All enzyme activity except invertase dominated in the soils containing Dalbergia and Cassia litters compared to the others. The seasonal effect was enzyme-dependent, with amylase and cellulase reaching peaks during the rainy season but invertase activity showed a reverse trend with lowest values in rainy season, except in Acacia soil, and protease activity was lowest in the soil containing Cassia and Dalbergia during the rainy season. Dehydrogenase activity was negligible in the soils containing Shorea and Acacia, but remained high with respect to Dalbergia and Cassia during all seasons. The decomposition pit study showed significant increase of soil nutrients with respect to litter types and intervals, except with respect to electrical conductivity. Cassia and Dalbergia litters enabled notable increase of soil nutrients than Shorea and Acacia. The soil enzyme activity, in general, increased with duration of litter decay, but microbial biomass C (MBC) decreased over time except in Shorea. Therefore, the enzyme rates normalized to the MBC indicated inverse relations for all enzymes, except in the soil containing Shorea. A positive relationship existed between MBC and soil respiration in Cassia, Acacia and Dalbergia. Analysis of variance revealed main effects of litter types for increasing protease, MBC and CO₂ output, and a main effect of intervals for enhancing enzymes other than cellulase. Rates of soil respiration were greater in soils contain Cassia and Dalbergia, and showed significant differences between litters and between intervals. All enzymes were significantly correlated with electrical conductivity, organic carbon and available phosphorus contents, and all enzymes except invertase were correlated with nitrate concentrations. The acidic soil pH did not affect enzyme activities, and soil nutrients exerted only weak effect on MBC and respiration. Our study showed that leaf litters of Cassia and Dalbergia trees improved the nutrient status and microbial activity in soil more so than Shorea and Acacia litters, and therefore, afforestation using Cassia and Dalbergia trees may be particularly suitable for soil restoration in tropical laterite wastelands.     

Microbial degradation and resynthesis of proteins during incubation of beech leaf litter in the presence of mineral phases

 Organic N constitutes more than 90% of total N in surface soil horizons. Amino acids, peptides and proteins represent the most abundant N species. There are indications that clay minerals influence the degradation of proteins, but little is known about the effect of oxides and hydroxides on protein decay. We therefore conducted an incubation experiment with mixtures of beech leaf litter and Fe oxide, Al hydroxide, Mn oxide or quartz sand. The protein amounts (quantified as α-NH₂–N) during the 498-day experiment were recorded. During the first 90–239 days, plant-derived proteins were decomposed, resulting in a decline of protein amounts to about 60% of the initial value. Later in the experiment, the protein amounts increased again to between 70% and 90% of the initial amount, because microbial resynthesis of proteins outweighed decomposition. The change from dominating decomposition to prevailing microbial resynthesis occurred when the microorganisms had to adapt to less favourable conditions and therefore built new, protein-rich biomass. Although the mineral phases did not influence protein decomposition initially, Fe oxide and Al hydroxide stabilized plant-derived proteins. Al hydroxide reduced protein resynthesis in the second phase of the experiment, probably due to a reduction of microbial activity. Mn oxide increased protein decomposition and lowered microbial resynthesis due to its oxidative properties. The mineral phases therefore resulted in a shift of the relative intensities of protein decomposition and microbial resynthesis. Received: 26 August 1998     

激活方法对水牛卵母细胞孤雌发育的影响

系统探讨了几种激活方法对水牛卵母细胞孤雌发育的影响。体外成熟 (IVM) 2 6 h的水牛卵母细胞经 7%乙醇(EH)、钙离子载体 (A2 3187)或离子霉素 (Ion)激活处理 5 min后 ,在 2 m mol/ L 6 -甲二氨基嘌呤 (6 - DMAP)中培养 3～4 h,然后再继续培养 6～ 11d,观察其胚胎发育情况。结果发现 ,5μmol/ L Ion激活处理的水牛卵母细胞分裂率显著高于 EH处理的卵母细胞 (6 3.0 % vs5 4 .2 % ,P<0 .0 5 ) ,其原核形成率也显著高于 EH处理的卵母细胞。激活处理前 ,无Ca2 培养液孵育时间的长短 ,对水牛卵母细胞孤雌发育无显著影响 (P>0 .0 5 )。如用 A2 3187激活处理水牛卵母细胞 ,其激活分裂率则随着浓度的升高而提高。从而表明 ,5 μmol/ L Ion可有效激活水牛卵母细胞     

植物生长调节剂在啤酒大麦上的增产效果试验初报

啤酒大麦使用W -HE生物表面活化剂、C -HE植物生长调节剂、喷施宝、丰产宝、生根粉能促进大麦生育前期的生长发育 ,增加分蘖 ,抑制后期的节间生长 ,有利于根系的发育和干物质的积累 ,对植株有较为明显的活化和调节作用 ,增产效果明显     

显微注射Ca^2＋激活小鼠M Ⅱ期卵母细胞

胞质内显微注入Ｃａ＾２＋可以激活小鼠Ｍ Ⅱ期卵母细胞，且随着卵龄的增加，激活率明显升高。卵龄小于１６ｈ（ｈＣＧ注射后）注射Ｃａ＾２＋引起的激活率很低，卵龄超过１８ｈ，激活率达到５０％以上。卵龄小于１６ｈ的卵母细胞，卵内注入超纯水未能激活卵母细胞，而在１８￣１９ｈ卵龄组，注射水的对照组激活率为３５％，但显著或极显著地低于注射Ｃａ＾２＋的试验组。激活前注射ＥＤＴＡ卵母细胞的激活率约为３０％，激活后注射     

微贮对秸秆在瘤胃NDF降解率的影响

试验采用3个重复对比设计，将玉米，小麦和稻草秸秆的微贮样与原样放置在永久瘘管牛体内，分别在6．12、24．36、48和72小时时间点取出样本进行NDF降解率试验测定分析，结果：秸秆处理样NDF在各个时间点的NDF瞬间降解率和有效降解率均极显著优于原样（P＞0．01）而玉米秸秆处理样与小麦和稻草处理样相比差异不显著（P＞0．05）。     

微生物肥对连作障碍土壤上芥蓝生长的影响

为解决土壤连作障碍问题,以不施微生物肥作对照,选用沃益多、德根贝2种微生物肥在芥蓝上进行设施栽培肥效对比试验。试验结果表明,施用微生物肥后芥蓝产量和经济效益均较对照有所增加,其中,沃益多处理的芥蓝产量增幅为10.07%,增值22 163元/hm2,德根贝处理的产量增幅为11.46%,增值25 817元/hm2,且达到显著差异,说明微生物肥对连作障碍有很好的缓解作用。     

Critical Discussion on Abortive Interspecific Crosses in Brassica

In most interspecific hybridization among diploid Brassica species (n = 8, 9, 10), few or no hybrids are obtained, and the three crosses of tetraploid (n = 17, 18, 19) by diploid species are usually only partially successful, especially in one of the reciprocal crosses. Such a cross-incompatibility complex is explained in this paper by a polar-nuclei activation (PNA) hypothesis which proposes two reversible functions, a normal reproduction and a sexual barrier, of double fertilization in higher plants. The first, i.e. the relative activating value (AV) of diploid species, was estimated as 1.0–3.5, whereas that of tetraploid species was 2.7–5.2. In addition, crosses with a polar nuclei activation index (AI) of about 15–87 % were compatible but those beyond the two limits were incompatible, 50 % of those showing normal seed development after self-pollination.     

Study on the Changes of the Amount of Microorganism and Microbial Biomass Carbon in Soil of Greenhouse Cropping

通过对四川省温江、双流两地不同设施种植年限土壤中微生物数量及其生物量碳的研究,结果表明,不同设施种植年限大棚土壤中的微生物数量是露地的1.63~3.50倍,且大棚土壤中好气性微生物总数随种植年限的增加有逐渐降低的趋势,三大微生物数量占微生物总数的比例中,细菌表现为随设施种植年限的增加而增加;放线菌和真菌表现为下降;露地土壤微生物多样性指数分别是设施年限为1a、2a和4a的大棚土壤的1.29倍、2.28倍和3.45倍,前者明显高于后者。随设施年限的增加,土壤微生物生物量碳、有机碳及微生物对有机碳的利用率都表现     

Functional diversity of microbial communities in the mixed boreal plain forest of central Canada

This study was designed to examine whether or not specific tree species (Picea glauca, Picea mariana, Pinus banksiana, Populus tremuloides), their post-fire stand age, or their position in a successional pathway had any significant effect on the functional diversity of associated soil microbial communities in a typical mixed boreal forest ecosystem (Duck Mountain Provincial Forest, Manitoba, Canada). Multivariate analyses designed to identify significant biotic and/or abiotic variables associated with patterns of organic substrate utilization (assessed using the BIOLOG™ System) revealed the overall similarity in substrate utilization by the soil microbial communities. The five clusters identified differed mainly by their substrate-utilization value rather than by specific substrate utilization. Variability in community functional diversity was not strongly associated to tree species or post-fire stand age; however, redundancy analysis indicated a stronger association between substrate utilization and successional pathway and soil pH. For example, microbial communities associated with the relatively high pH soils of the P. tremuloides-P. glauca successional pathway, exhibited a greater degree of substrate utilization than those associated with the P. banksiana-P. mariana successional pathway and more acidic soils. Differences in functional diversity specific to tree species were not observed and this may have reflected the mixed nature of the forest stands and of their heterogeneous forest floor. In a densely treed, mixed boreal forest ecosystem, great overlap in tree and understory species occur making it difficult to assign a definitive microbial community to any particular tree species. The presence of P. tremuloides in all stand types and post fire stand ages has probably contributed to the large amount of overlap in utilization profiles among soil samples.