长寿花快繁及试管苗开花研究

[目的]探讨长寿花试管苗培养及诱导开花的关键技术。[方法]以长寿花带芽茎段为材料,研究HgC l2消毒时间、植物生长调节剂配比对外植体污染率、增殖效果及试管苗开花的影响。[结果]HgC l2消毒8 m in效果最好,污染率为20%,明显低于不消毒的77.77%;培养基中6-BA添加量为2.8 mg/L时,试管苗明显高于添加量为1.0、1.5、2.1 mg/L的处理,IAA对丛芽增殖影响不明显,但添加量过高抑制丛芽增殖。增殖培养基中植物生长激素的最佳组合为：0.30 mg/L IAA＋2.1 mg/L 6-BA＋0.6 mg/L GA3;NAA添加量为2.0-4.5 mg/L时,均能诱导花芽分化,诱导率为5.5%-35.6%,单独使用GA3不能诱导试管苗开花,但GA3与NAA配合使用可使试管苗提前15 d开花。[结论]诱导长寿花试管苗增殖及开花的最佳培养基分别为MS＋0.30 mg/L IAA＋2.1 mg/L 6-BA＋0.6 mg/LGA3和MS＋2.5 mg/L NAA。     

长寿花离体繁殖技术研究

用长寿花的带芽茎段为外植体,进行了外植体的建立、增殖、生根及移栽研究。结果表明:芽启动培养基以MS+6-BA0.5mg/L+NAA0.1mg/L为宜,启动率达100.0%。增殖培养中,6-BA与NAA、GA配合使用增殖效果好,外植体培养在MS+6-BA2.0mg/L+NAA0.2mg/L+GA30.1mg/L上可增殖9.3倍。采用MS+6-BA1.0mg/L+GA30.1mg/L+AC2.0g/L的半固体培养基,可使长寿花增殖与生根同步进行,每个外植体平均可产生5.5个芽,生根率达100.0%。试管苗移栽入土成活率达100.0%。     

水培条件对长寿花的影响

本试验以黄色重瓣长寿花(kalanchoe blossfeldiana)为材料,研究了花肥种类、换液时间、杀菌剂及它们共同作用对水培长寿花生长的影响,旨在研究出简易便于推广的长寿花水培方式方法。     

大叶落地生根细胞周期蛋白依赖激酶KdCDK基因克隆及表达分析

为了更好理解大叶落地生根中细胞周期调控的分子机制,利用RACE-PCR技术从大叶落地生根中克隆了1个新的基因KdCDK。该基因编码295个氨基酸残基,分子量为34.12kDa,等电点为6.15,其开放阅读框长度为888bp。其蛋白与笋瓜CDK蛋白的同源性最高,属于CDKA类蛋白家族。实时荧光定量PCR分析表明,该蛋白基因在大叶落地生根的根中表达量最高,且受渗透胁迫(甘露醇)的诱导上调表达。从大叶落地生根中克隆出KdCDK基因,为将来该基因的功能研究打下了基础。     

An overview of potentially life-threatening poisonous plants in dogs and cats

Objectives: This article discusses the toxicity of the most common poisonous plants known to cause life‐threatening systemic effects or death in dogs and cats as reported to the ASPCA Animal Poison Control Center (APCC). Data sources: The article presents an overview of geographic distribution, toxic principle, clinical signs, clinical chemistry alterations, and treatment of intoxication as reported in the literature and based upon data retrieved from the APCC between January 2001 and December 2003. Summary: Most plant exposures in dogs or cats result in mild to moderate signs of vomiting and diarrhea while liver, kidney, central nervous system, or cardiovascular effects are rare. Some garden or household plants can cause serious systemic effects or death when a small amount of plant material has been ingested. Based on APCC records, the most frequently reported poisonous plants causing serious systemic effects include Lily (Lilium and Hemerocallis spp.), Azalea (Rhododendron spp.), Oleander (Nerium oleander), Sago palm (Cycas spp.), Castor bean (Ricinus communis), Kalanchoe (Kalanchoe spp.), and Autumn Crocus (Colchicum autumnale). Conclusion: Despite the variety of toxins present in the plants listed above, early clinical signs of toxicosis in dogs and cats can be nonspecific and can include vomiting, lethargy, anorexia, salivation, or diarrhea. Cardiac arrhythmias may be present with oleander, azalea, or kalanchoe ingestion. Evidence of liver or kidney damage (Cycas, Lilium, Hemerocallis spp., Ricinus communis) may occur 1–2 days after the exposure. Treatment consists of early decontamination and supportive care and may vary according to the type of plant involved and clinical signs present.     

褐斑伽蓝的组织培养与快速繁殖

试验选材为景天科多肉植物褐斑伽蓝,在建立起再生系统后,分别以茎段和叶片为试验材料,研究了不同外源激素配比对其器官分化的影响,从中筛选出适合褐斑伽蓝快速繁殖的最适培养基。     

红花长寿花愈伤组织的诱导和植株再生研究

350002 福建省福州金山福建农林大学园艺植物生物工程研究所     

重瓣长寿花叶片组织培养及植株再生的研究

摘要:以重瓣长寿花幼嫩叶片为试材,筛选各个培养阶段的培养基配方,研究重瓣长寿花的快速繁殖技术。结果表明: 0.1％升汞灭菌4min,外植体污染率为12.5％,死亡率2.5%,效果最佳;愈伤组织诱导培养基以MS+6-BA1.0 mg／L+NAA0.1～0.3 mg／L最佳,不定芽分化和增殖的最适培养基为MS+6-BA0.5 mg／L+NAA0.1 mg／L,生根培养基用1/2MS+NAA0.5mg／L最好,试管苗移栽成活率达99％。     

一种适于繁殖东亚小花蝽的产卵植物——寿星花

比较了3种植物组织（寿星花嫩茎、黄豆芽和燕子掌叶片）作为东亚小花蝽产卵植物的适合性。东亚小花蝽对这3种产卵植物无明显的选择性，在单雌产卵量、产卵前期、产卵期和雌成虫命等指标上也明显差异。以米蛾卵和桃蚜为饲料，东亚小花蝽单雌产卵量平均为42．1粒、产卵前期3．3d、产卵期9．7d、雌成虫寿命15．0d。产于寿星花嫩茎上的小花蝽卵孵化率高，在4－6℃条件下冷藏13d不影响孵化率。较之黄豆芽及燕了掌叶片，寿星花嫩茎具有能满足若虫发育期间对植物的需求和植件易于栽种的优点，是一种较好的小花蝽产卵植物。     

不同培养条件下长寿花叶片再生体系的构建

（河南农业职业学院植物科学系，河南中牟 451450）