水稻纹枯病菌胞外蛋白酶分离纯化及部分特性分析

[目的]为深入研究水稻纹枯病菌的致病机理奠定基础。[方法]水稻纹枯病菌（Rhizoctonia solaniKhn）发酵液经硫酸铵沉淀,用离子交换柱层析、疏水层析和分子筛层析方法分离纯化胞外蛋白酶。[结果]从水稻纹枯病菌发酵液中分离纯化到一个分子量约为49.5ku的胞外蛋白酶。其活性最适温度为30℃,最适pH为6.4。Zn^2＋、Fe^3＋、Cu^2＋对酶活性有抑制作用,Mg^2＋、Mn^2＋对酶活性无影响,Ca^2＋在低浓度下对酶活性有激活作用。[结论]水稻纹枯病菌可产生胞外蛋白酶,该胞外蛋白酶与水稻纹枯病菌致病性的关系还有待深入研究。     

薇甘菊萎蔫病毒感染对薇甘菊光合特性和4种酶活性的影响

为明确薇甘菊萎蔫病毒(Mikania micrantha wilt virus,MMWV)对薇甘菊(Mikania micrantha)叶片生理生化的影响,探讨了MMWV侵染对薇甘菊叶片超氧化物岐化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)和苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)活性及叶绿素总含量和光合特性的影响.结果表明:MMWV感染薇甘菊16d内叶片的SOD活性均比对照组高,其中第16天达最大值,但接种后第24和32天SOD活性分别比对照低了13.28％和25.37％;POD活性在接种后16-32d均显著高于对照.PPO和PAL变化趋势相似,在接种后第8天这两个酶的活性分别比对照高了77.75％和23.58％,而在第32天分别比对照减少了14.27％和20.53％.随着侵染时间的增加,感病薇甘菊叶片中叶绿素含量减少,叶绿素a/b值逐步降低;同时最大净光合速率(Pmax)和光饱和点(LSP)分别比健康对照减少了32.34％和12.52％,而对暗呼吸速率(Rd),光补偿点(LCP)和表观量子效率(AQY)无显著影响.表明MMWV侵染可减低薇甘菊叶片光合作用的效率.     

扶贫产业开发新思路:打造跨区域扶贫产业区

《中国农村扶贫开发纲要(2011-2020年)》把全国贫困地区划分为14个连片特困地区,力求打破以往的点式扶贫和区域限制。但是,扶贫产业发展仍旧受到行政区域的限制,各自为营。为此,从扶贫对象转变的视角梳理了中国贫困治理的历史演进逻辑,并提出打造跨区域扶贫产业区的扶贫产业开发新思路,即在充分尊重连片特困区的资源特点、产业特点的基础上,完全立足于产业发展,打破区域界线,实现资源的统一配置,提高规模效益、资源配置效率和扶贫效果。但是,当前打造跨区域扶贫产业区面临着内涵的科学界定、分工与协调机制欠缺、区内产业发展参差不齐、地方资金配套标准难以统一等现实困境。因此,需要把跨区域扶贫产业区建设作为中央单列扶贫项目管理、探索"联合体发展模式"、普惠与特惠政策相结合以及创新参与式产业扶贫模式等措施,促进跨区域扶贫产业区建设和发展。     

不同钾肥施用水平对木薯产量影响试验初报

为探索不同钾肥施用水平对木薯产量的影响,笔者在藤县进行同量基肥、不同量钾肥追肥施用的研究试验。试验结果表明：追施不同量的钾肥均能增加木薯的产量,但对作物的农艺性状基本无影响;从综合效益出发,以追施氯化钾225kg／hm^2产生的综合效益最好。     

Knockdown of HMGA2 gene inhibits TGF-β1-induced human embryonic lung fibroblast growth and collagen synthesis

AIM: To investigate the effects of high mobility group A2(HMGA2) gene knockdown on the cell viability, apoptosis, collagen synthesis and oxidative stress of human embryonic lung fibroblast (HELF) induced by transforming growth factor-β1 (TGF-β1). METHODS: The HELF were divided into blank group, TGF-β1 group,negative control (NC) group and HMGA2 siRNA(si-HMGA2) group. The protein levels of HMGA2, AKT and p-AKT were determined by Western blot. The cell viability and apoptotic rate was analyzed by MTT assay and flow cytometry,respectively. The mRNA expression of collagen I (COL-Ⅰ) and COL-Ⅲ was detected by RT-qPCR. DCFH-DA was used to detect the content of reactive oxygen species (ROS). RESULTS: Compared with blank group, the protein levels of HMGA2 and p-AKT, the cell viability, the mRNA expression of COL-Ⅰ and COL-Ⅲ in TGF-β1 group were significantly increased, but the apoptotic rate and ROS level were significantly decreased (P<0.05). Compared with TGF-β1 group, the protein levels of HMGA2 and p-AKT, the cell viability, the mRNA expression of COL-Ⅰ and COL-Ⅲ in si-HMGA2 group were significantly decreased, but the apoptotic rate and ROS level were significantly increased (P<0.05). CONCLUSION: Knockdown of HMGA2 gene expression decreases the viability and collagen synthesis, and promotes apoptosis and ROS production of human embryonic lung fibroblasts induced by TGF-β1. The mechanism may be related to down-regulation of PI3K/AKT signaling pathway.     

A new method for measuring microbial biomass nitrogen in soils: Direct extraction after toluene treatment

Abstract

Phenolic compounds occurring naturally are reducing agents which react with hydrous Fe oxide and Mn oxides (Lehmann et al. 1987). Diphenols such as hydroquinone, resorcinol, and catechol were oxidatively darkened by the presence of hydrous Fe oxide though the degree of darkening was much less pronounced than that by Mn oxides (Shindo and Huang 1984). Okazaki et al. (1976) suggested that polyphenols are one of the materials responsible for the dissolution of Mn in a paddy soil under reducing conditions.     

Interaction Between Salinity Stress and Verticillium Wilt Disease in Three Pistachio Rootstocks in a Calcareous Soil

ABSTRACT

The interaction between soil salinity and infection caused by Verticillium dahliae was studied in pistachio (Pistacia vera) in a greenhouse experiment. Treatments consisted of 0, 1400, 2800, and 4200 mg sodium chloride (NaCl) kg^{? 1} soil and three rootstocks (Sarakhs, Badami, and Qazvini cultivars). They were gradually exposed to salinity stress before and/or after root inoculation with a water suspension of 10⁷ conidia/mL of a pistachio isolate of V. dahliae. Salt stress significantly increased rootstock shoot and root colonization by V. dahliae. All rootstocks were susceptible to V. dahliae, but symptoms of the disease appeared earlier in Sarakhs, a salt sensitive cultivar. Moreover, salinity and V. dahliae interaction increased the concentrations of sodium (Na), potassium (K) and chloride (Cl), but decreased the K/Na ratio in all rootstocks. Shoot and root tissues of inoculated Sarakhs and Qazvini (a salt tolerant) contained the highest and the lowest concentrations of Na, K,and Cl, respectively. In salinity treatments, shoot and root dry weight of all rootstocks decreased as compared with controls. Sarakhs showed smaller shoot and root dry weight than Qazvini and Badami. Also, increasing the NaCl level increased accumulation of Na, K, and Cl in shoot and root of the rootstocks. Sarakhs showed higher concentrations of ions in the shoot and root. Based on shoot and root dry weights and ion accumulation, Sarakhs and Qazvini were susceptible and tolerant to salinity, respectively.     

Dry matter and primary nutrient accumulation in soybeans as affected by combined Nitrogen levels

Nodulating and non‐nodulating soybeans were grown on a Alfic Udipsamment and a Typic Hapludoll amended with 10 or 100 kg N/ha. Tissue and grain samples were analyzed to determine N₂‐fixation, dry matter, and N, P, and K accumulation. Highest grain yields were associated with the highest levels of N₂‐fixation and N and K accumulation in grain. The largest dry matter production was by nodulating plants grown on a high soil N regime. Nodulating plants accumulated more grain and tissue N, P, and K than non‐nodulating plants. Nitrogen stress increased P concentrations in both grain and tissue and decreased harvest indices.     

Influence of thigmic stress or chlormequat chloride on tomato morphology and elemental uptake

Root dry weight and leaf number were not affected by thigmic stress or chlormequat chloride. Shoot dry weight, shoot:root dry weight ratio, shoot height, leaf area, and root surface area were decreased by both thigmic stress and chlormequat chloride. However, root length was decreased and root radius increased only by chlormequat chloride. Total element uptake was decreased by both thigmic stress and chlormequat chloride. In the shoot and root, N was not affected, P was increased in the shoots, K decreased in the shoots but increased in the roots, and Mg decreased in the roots as a result of thigmic stress and chlormequat chloride. Whereas, Ca was decreased in the roots and Mg increased in the shoots by chlormequat chloride. The uptake of N, P, Ca, or Mg was not affected by thigmic stress or chlormequat chloride, however, K uptake per root surface area decreased. Thigmic stress decreased K in both the upper and lower stem but did not affect leaf concentration of K. However, chlormequat chloride decreased K in both the stem and leaves. Thigmic stress because of the role of K in cell elongation. Chemical name used: (2‐chloroethyl)trimethyl‐ammonium chloride (chlormequat chloride).     

温度湿度及种球茎大小对花魔芋有性繁殖的影响

有性繁殖是促进魔芋种植业发展和品种改良的有效途径，引起了极大的兴趣。本文研究117个花魔芋（Amorphophallus konjac K. Coch)单株花粉成熟后连续5d的温度、湿度以及花芽种球茎对其有性繁殖的影响，结果表明：授粉期间的温度和湿度对其自花授粉结实影响大，结实株花粉成熟后连续5d的平均温度和湿度分别为24℃和64%，均显著高于不实株，高温高湿有利于散粉和花粉的萌发从而完成受精；花芽种球茎大小对有性繁殖也有影响，结实株平均种球茎重显著高于不实株，且与单株结实浆果数呈极显著的正相关（r=0.6096, N=29），因此，宜选用花芽球茎大的用于魔芋有性繁殖。