全文获取类型
收费全文 | 1967篇 |
免费 | 118篇 |
国内免费 | 189篇 |
专业分类
林业 | 67篇 |
农学 | 721篇 |
基础科学 | 1篇 |
205篇 | |
综合类 | 477篇 |
农作物 | 194篇 |
水产渔业 | 106篇 |
畜牧兽医 | 268篇 |
园艺 | 128篇 |
植物保护 | 107篇 |
出版年
2024年 | 6篇 |
2023年 | 23篇 |
2022年 | 40篇 |
2021年 | 62篇 |
2020年 | 64篇 |
2019年 | 81篇 |
2018年 | 52篇 |
2017年 | 63篇 |
2016年 | 75篇 |
2015年 | 94篇 |
2014年 | 101篇 |
2013年 | 101篇 |
2012年 | 93篇 |
2011年 | 119篇 |
2010年 | 118篇 |
2009年 | 139篇 |
2008年 | 119篇 |
2007年 | 120篇 |
2006年 | 128篇 |
2005年 | 112篇 |
2004年 | 98篇 |
2003年 | 85篇 |
2002年 | 60篇 |
2001年 | 66篇 |
2000年 | 49篇 |
1999年 | 43篇 |
1998年 | 33篇 |
1997年 | 20篇 |
1996年 | 26篇 |
1995年 | 22篇 |
1994年 | 9篇 |
1993年 | 8篇 |
1992年 | 14篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1955年 | 2篇 |
排序方式: 共有2274条查询结果,搜索用时 15 毫秒
141.
In the small, agricultural, artificially drained Orgeval watershed δ15N values of leached nitrates and soil organic nitrogen were found to be significantly higher than the primary nitrogen (N) sources from which they are derived, namely, synthetic fertilizers, atmospheric deposition, and symbiotic or nonsymbiotic N2 fixation (all with δ15N close to zero). In vertical soil profiles, the δ15N of organic N increased with depth, reaching higher values (up to 8‰) particularly at stations that were frequently waterlogged as judged from ochre iron traces, such as downhill field sites or in riparian buffer strips. Nitrification, volatilization, and denitrification are the main fractionating processes able to modify the isotopic composition of soil N. Using a newly designed algorithm for calculating the equilibrium isotopic composition of all soil N species, resulting from the average annual balance of their transformations, we show that the observed trends can be explained by the action of denitrification. We suggest that the isotopic composition of soil organic N can be used as a semiquantitative indicator of the intensity of denitrification integrated over century-long periods. 相似文献
142.
Marta Novo Ana Almodóvar Rosa M. Fernández Mónica Gutiérrez Darío J. Díaz Cosín 《Pedobiologia》2010,53(6):375-379
Endogeic earthworms are difficult study subjects due to the cryptic medium in which they live; thus, only the behaviour of epigeic and anecic earthworms has been studied before. We used microsatellite markers as a tool to elucidate the mate choice processes of Hormogaster elisae, an endogeic earthworm. It was shown to normally mate with two partners, preferably of the same size that are found in close proximity thereby eliminating the need for long-distance dispersion, which could explain the previously observed high genetic differentiation between populations. The genetic analyses of the sperm within each of its four spermathecae showed a uniform distribution with no signs of differential storage of sperm from different partners. 相似文献
143.
Chemical taxonomy based upon the composition of lipids is widely applied to investigate microbial communities and fatty acids have recently been employed to connect soil microbial and faunal food webs as well as to elucidate functional groups at higher trophic levels. The additional use of compound-specific isotopic analysis of 13C/12C ratios in fatty acids allows assessing specific trophic links and belowground carbon fluxes. In this review systematic patterns and processes underlying variations in the composition of fatty acids and their 13C/12C ratio are described. The emphasis is on biomarker fatty acids, their incorporation and modification, effects of pool size, and analytical methods. Further development of the application of fatty acid profiling to soil ecology should include both advances in experimental research and growth of theory. Accordingly, areas in which future experimentation can lead to progress in soil food web analysis are identified. Overall, combining fatty acid biomarker and their isotopic ratios will allow detailed insight into belowground trophic interactions. 相似文献
144.
Immunohistochemical characterization of canine prostatic carcinoma and correlation with castration status and castration time 总被引:2,自引:0,他引:2
K. U. Sorenmo M. Goldschmidt F. Shofer C. Goldkamp J. Ferracone 《Veterinary and comparative oncology》2003,1(1):48-56
The purpose of this study was to characterize canine prostate cancer using immunohistochemical staining specific for acinar and urothelial/ductal tissue and correlate these results with the dogs' castration status/castration time. Seventy dogs with prostate cancer were included, 71% were castrated and 29% were intact. Compared with an age‐matched control population, castrated dogs were at increased risk of prostate cancer, odds ratio 3.9. Immunohistochemical staining was performed on 58 cases. Forty‐six of the 58 stained positive for cytokeratin 7 (CK 7) (ductal/urothelial origin) and one of the 58 stained positive for prostate‐specific antigen. Dogs with CK 7‐positive tumours were younger when castrated than dogs with CK 7‐negative tumours, 2 versus 7 years (P = 0.03); dogs castrated at ≤2 years of age were more likely to be CK 7‐positive (P = 0.009). These results show that most canine prostatic carcinomas are of ductal/urothelial, androgen‐independent origin. This is consistent with the epidemiological findings, showing increased risk in castrated dogs. Canine prostate cancer may, therefore, not be a realistic model for the human disease. 相似文献
145.
Generalized marker regression and interval QTL mapping methods for binary traits in half-sib family designs 总被引:3,自引:0,他引:3
H. N. Kadarmideen & J. C. M. Dekkers 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2001,118(5):297-309
A Generalized Marker Regression Mapping (GMR) approach was developed for mapping Quantitative Trait Loci (QTL) affecting binary polygenic traits in a single-family half-sib design. The GMR is based on threshold-liability model theory and regression of offspring phenotype on expected marker genotypes at flanking marker loci. Using simulation, statistical power and bias of QTL mapping for binary traits by GMR was compared with full QTL interval mapping based on a threshold model (GIM) and with a linear marker regression mapping method (LMR). Empirical significance threshold values, power and estimates of QTL location and effect were identical for GIM and GMR when QTL mapping was restricted to within the marker interval. These results show that the theory of the marker regression method for QTL mapping is also applicable to binary traits and possibly for traits with other non-normal distributions. The linear and threshold models based on marker regression (LMR and GMR) also resulted in similar estimates and power for large progeny group sizes, indicating that LMR can be used for binary data for balanced designs with large families, as this method is computationally simpler than GMR. GMR may have a greater potential than LMR for QTL mapping for binary traits in complex situations such as QTL mapping with complex pedigrees, random models and models with interactions. 相似文献
146.
147.
148.
149.
Jerzy Drzewiecki 《Euphytica》2001,119(3):279-287
Wheat-rye translocation line (2BS/2RL) has been developed for resistance to biotype L of Hessian fly and agronomically useful
traits. AFLP analysis using 64 primer pairs was conducted in order to identify 2RL-specific polymorphisms between “Coker 797”
(non-2RL), near-isogenic line (NIL) carrying 2RL, and “Hamlet”. Nine primer combinations identified twelve reproducible polymorphic
fragments in the NIL carrying 2RL. These twelve fragments were cloned and sequenced with an aim towards converting AFLP markers
into sequence tagged sites (STS). A comparison of the 12 sequences with non-redundant accessions in the NCBI database using
the BLAST search option indicated that one fragment of approximately 200 bp in length (amplified using primer combination
E+AAC / M+CTA) was highly homologous with the rye-specific repetitive sequence R173-1 and Wis-2-lA, a retrotransposon-like
element in wheat. Two STS primers (SJ07 and SJ09) out of twelve STS primer sets enabled the detection of polymorphisms between
Coker 797 and NIL carrying 2RL. In order to verify whether the polymorphism detected by primers SJ07 and SJ09 was in fact
the result of the presence of 2RL, additional plant material was examined. Amplified products of about 260 bp fragment with
the SJ07 primer set were generated in rye cvs.“Chilbohomil” and “Jochunhomil”, triticale experimental line Suwon 15, and wheat
experimental line K-14 (1AL/1RS & 2BS/2RL), as well as NIL carrying 2RL and Hamlet, but not in Coker 797 (non-2RL), “Keumgangmil”
(non-translocation wheat), KS92WGRC17 (PI592729 /;/ 6BS/6BL-6RL), KS92WGRC19 (P1592731 /;/ 4BS/4BL-6RL), “TAM200” (1AL/1RS),
and “Siouxland” (1BL/1RS). Our data suggest that primer set SJ07 amplifies a “2RL-specific” fragment of diagnostic value.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
150.
Intraspecific olive diversity assessed with AFLP 总被引:6,自引:0,他引:6
F. Sanz-Cortés D. E. Parfitt C. Romero D. Struss G. Llacer M. L. Badenes 《Plant Breeding》2003,122(2):173-177
Amplified fragment length polymorphism (AFLP) was used to study diversity within and among Spanish olive varieties. A high degree of diversity was found among the varieties present in each growing region. Olive oil production and quality relies on appropriate cultivar selection as well as good orchard management. Production based only on a few superior cultivars would result in improved yield, oil quality, and production management. Amplified fragment length polymorphism were evaluated as a tool to identify the intraspecific and intravarietal diversity of olive. Amplified fragment length polymorphism analysis of 38 accessions belonging to 10 cultivars using six primer combinations produced 106 polymorphic bands. Results were analyzed for similarity among accessions via unweighted pair‐group means cluster analysis, resulting in 10 clusters corresponding to named variety designations. Similarity among varieties ranged from 0.60 to 0.72. Diversity within varieties was identified. Similarity within named varieties (accessions with the same varietal name) ranged from 0.75 to 0.96. Differences in several markers were found among 34 accessions. Intravarietal diversity was shown to exist within the Spanish olive varieties grown in the region surrounding Valencia. 相似文献