杨梅自发气调保鲜研究初报

对3种气调保鲜箱贮藏环境的气体成分变化及对杨梅保鲜效果进行了研究。试验结果表明,在0℃冷藏条件下,使用气调保鲜箱杨梅适宜贮藏时间为20天,保鲜效果明显好于对照;气调箱-2效果最好。气调箱-2在贮藏6天时O2、CO2含量达到平衡;在贮藏12～15天时CO2含量达到峰值20.65%,O2含量接近2%,该环境条件有利于杨梅贮藏保鲜。     

成熟期库尔勒香梨理化指标变化规律及相关性研究

为了研究库尔勒香梨成熟过程中理化指标的变化规律,探求成熟期库尔勒香梨主要理化指标间的相关性,以成熟期库尔勒香梨为研究对象,研究库尔勒香梨果实硬度、叶绿素含量、SSC(可溶性固形物含量)和维生素C含量的变化规律;采用相关分析和回归分析的方法,应用Spss软件和Excel软件对指标均值以及各指标间相关性进行了分析,并对试验结果进行了实验验证。研究结果表明:在成熟期内,库尔勒香梨果实理化参数变化范围分别为:硬度4.23~7.99 kg/cm2、叶绿素3.17~6.54 mg/100 g、SSC11.54%~15.41%、维生素C 1.9~4.2 mg/1 0 0 g;随着成熟的深入,果实硬度逐渐降低、叶绿素含量逐渐减少、SSC逐渐增加、维生素C含量逐渐增加;各指标均值在0.01水平上显著相关。回归分析过程中选取最优的拟合方式,并建立相应的数学模型,果实硬度和SSC与叶绿素含量和维生素C含量间决定系数均大于0.95,验证试验预测值与测量值误差小于3.2 1%,在库尔勒香梨成熟过程中可应用果实硬度和SSC对叶绿素和维生素C含量进行预测。研究结果能够为库尔勒香梨成熟度评价和分级提供参考。     

香梨幼林园在不同灌溉条件下生长的动态变化相关分析

在香梨3年生幼林园内,进行了常规沟植、渠道内外二侧种植的土壤贮水量和树木新梢生长量、叶片生长状况变化的测定。结果表明,渠外行的土壤贮水量和生长状况均好于沟植和渠内行。揭示了影响树木生长的不同灌溉条件的土壤水分状况和过程。提出了生产中要提倡渠外行栽种香梨,保持沟植香梨,放弃渠内行栽植香梨的生产模式理论依据。     

基于耕地机犁体自由曲面的数控铣削加工

耕地机犁体曲面是空间任意的曲面。设计合理的犁体曲面,应采用CAD/CAM软件进行实体造型,曲面设计成高速梨曲面,既可以降低无效阻力,又能减轻犁的质量,增强曲面的光滑程度。为此,采用数控加工中心对耕地机犁体空间自由曲面进行铣削加工,选择不同的加工方式和合理的参数,对粗加工时间和精加工结果进行对比分析。结果表明:流线式粗加工和环绕等距式精加工组合有更高的加工效率和更好的质量,能使耕地机减少抛扔的能量消耗,提高耕地机零部件的加工效率。     

Effect of CO2 enrichment on fruit growth and quality in Japanese pear (Pyrus serotina Reheder cv. Kosui)

Six year-old Japanese pear (Pyrus seratina Reheder cv. Kosui) trees grafted on P. serotina cv. Nihonyamanashi were grown in containers filled with Granite Regosol under glasshouse conditions. At different stages of fruit growth, pear trees were exposed to an elevated CO₂ concentration (130 Pa CO₂ ) along with a control (35 Pa CO₂). For one group of plants, CO₂ enrichment was applied for 79 d from 52 d after full bloom (DAB) to fruit maturity (long-term CO₂ enrichment) and for another group the same treatment was applied for 35 d from 96 DAB to fruit maturity (short-term CO₂ enrichment). The effects of the elevated CO₂ concentration on vegetative growth, mineral contents, and fruit production and quality were examined. Long-term CO₂ enrichment enhanced vegetative growth, without any significant effect on the mineral contents in either flower bud or fruit except for a remarkable increase in the K content. Long-term CO₂ enrichment increased the fruit size and fresh weight, but had no significant effect on the fruit quality. On the other hand, the short-term CO₂ enrichment did not induce any significant change in the fruit size but increased the fruit sugar concentration. Along with the reduction of the sorbitol concentration in fruit, the fructose and sucrose concentrations increased and these changes occurred earlier at elevated CO₂ than at ambient CO₂ concentrations. From these results, we concluded that the effect of CO₂ enrichment on fruit growth varies depending upon the growth stages of fruit: during the initial and fruitlet stages when fruit expansion occurs, CO₂ enrichment increases the fruit size, whereas, during maturation when fruit expansion has slowed down and sugar accumulation in fruit is active, it increases the fruit sugar concentration.     

梨汁生产配方的研究

采用L_9(3~3)正交试验研究梨汁的配方,在单因素试验的基础上,确定生产出风味纯正的梨汁的最佳配方为:梨加入量2.5 kg,糖质量分数6.5%,糖水添加量6.0 kg。     

软儿梨冷冻贮藏过程酚类物质与多酚氧化酶的区域化分布

为研究冷冻贮藏中不同部位酚类物质和多酚氧化酶的分布特征,以青海省地方梨优良品种‘软儿梨’果实为研究材料,采用高效液相色谱法和比色法测定软儿梨果实后熟(室温)、冻藏(-18℃)和解冻(室温)3个时期的果皮、果肉中总多酚、绿原酸的含量和多酚氧化酶活性。结果表明:软儿梨果实后熟后,酚类物质果肉?果皮,多酚氧化酶则果皮?果肉;冷冻贮藏过程中,不同部位的酚类物质和多酚氧化酶均呈现不同程度的升高,但绿原酸在果皮急剧下降、果肉中略有增加。解冻后总体呈下降趋势,2个部位总酚、多酚氧化酶下降明显,而果皮中的绿原酸变化不大。研究表明:软儿梨果实后熟后,酚类物质和多酚氧化酶分布呈现区域化,冷冻贮藏加剧了酚类物质和多酚氧化酶活性的积累,导致了软儿梨冷冻贮藏后果皮先于果肉发生了褐变。     

‘新世纪梨’ב崇化大梨’F1代分子遗传连锁图谱的构建

研究目的在于为以后控制重要农艺性状的QTL定位、梨分子标记辅助育种及品种改良提供基础理论。利用‘新世纪梨’ב崇化大梨’杂交得到的210 株F1代实生苗为作图群体,对分离群体进行了ISSR、SRAP、SSR 标记的多态性检测,共得到154 条多态性条带,其中偏离孟德尔遗传比例的含21.4%(P＜0.01)。应用JoinMap 4.0 软件对154 个多态性条带进行遗传连锁分析,构建了一张包括9 个SSR标记,79个SRAP标记,8个ISSR标记,合计96个标记分属于14个连锁群的遗传图谱,图谱总长度为1530 cM,平均图距为16.1 cM,最大的连锁群含有64个标记,最小遗传距离小于0.1 cM。     

一种利用流式细胞术鉴定梨倍性的方法

流式细胞术鉴定植物染色体倍性的方法广泛应用于果树多倍体育种中,然而相关流式细胞术鉴定梨染色体倍性的方法还没有系统的研究。以梨树叶片为试材系统优化了各个流式细胞术关键实验节点,结果表明：实验材料选取当年春季新梢第3~5 片梨树嫩叶,尤其是大棚新梢嫩叶最好;叶片前处理时用蒸馏水、去离子水依次洗净叶片表面后,再用去离子水浸泡10 min;使用WPB解离液对梨叶片细胞核进行提取后,用500 目滤膜过滤2 次,离心1 次后用流式细胞仪进行检测可得到理想的实验结果。该方法步骤简单、结果可靠,是一种快速、高效的梨染色体倍性鉴定的方法,可广泛用于梨树多倍体鉴定。     

Combined effects of sodium chlorite dip treatment and chitosan coatings on the quality of fresh-cut d’Anjou pears

This study evaluated the effects of sodium chlorite (SC) alone and its sequential treatment with edible coatings on browning inhibition and quality maintenance of fresh-cut d’Anjou pears. Edible coatings were prepared from chitosan (CH) and its water-soluble derivative carboxymethyl chitosan (CMCH), separately. Pear wedges were immersed in SC solution, followed by coating with CH or CMCH solutions. The samples were packed in unsealed bags and stored at 4 °C for subsequent color, firmness, and weight loss measurement. The effects of the SC and coating treatments on polyphenol oxidase (PPO) inhibition and microbial inactivation were also evaluated. Results indicated that SC exhibited significant (P < 0.05) inhibition of browning and PPO activity. The SC treatment was also strongly effective in inactivating Escherichia coli O157:H7 on pear slices. Coating SC-treated pear slices with CH adversely affected the quality of pear slices by accelerating the discoloration of cut surfaces and increasing the PPO activity. On the contrary, coating SC-treated samples with CMCH significantly prevented the browning reaction and inhibited PPO activity. In addition, SC and CH/CMCH coatings maintained tissue firmness and did not affect weight loss. Our study may provide a scientific basis for the use of SC + CMCH treatment as an alternative preservation treatment for fresh-cut fruits and vegetables.