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91.
河南省承接产业转移的现状和特点 总被引:1,自引:0,他引:1
本文就河南省承接产业转移的需要,把快速调整产业结构、实现跨越式发展的现实,区域间的产业梯次分布转移的前提,河南省承接产业转移的时机条件,承接产业转移的势头,以及面临的差距、面临的竞争形势、区域竞争形势进行了阐述。 相似文献
92.
Hugh Riley 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):122-134
Trials were performed with early and semi-early potatoes to test the effects of nitrogen (N) fertilizer level (0-160 kg N ha-1) and timing (all at planting versus half then and half either soon after emergence or 3 weeks later). All seven trials with earlies were irrigated as required, whilst different irrigation regimes (moderate versus intensive) were compared in two trials with semi-earlies. No benefit was derived from splitting the N application. Haulm growth and N uptake increased in all cases almost linearly up to the highest N level, but tuber yield did not respond in the same way. The optimum N level was 80 kg N ha-1 for a yield of 15 Mg ha-1, rising to 120 kg N ha-1 for a yield of 40 Mg ha-1. Tuber quality was lowered by the use of excess N fertilizer, particularly in the case of earlies. The quantity of mineralised N present in the soil after harvest rose sharply with above optimum fertilizer use, and the amount of N present in crop residues also increased. The likely leaching after early potatoes was estimated to be up to 80 kg N ha-1. The proportion of fertilizer N which was not accounted for in either tuber yield, crop residues or mineral N in soil was 26% in earlies and 38% in semi-earlies. 相似文献
93.
Degradation rates of pyrogenic carbon (PyC) under natural environmental conditions are largely unknown. Here we present results from a field experiment monitoring the change in mass, C- and N concentrations of a variety of charcoal types in a Norwegian boreal forest over a period of 20 months. The charcoal types represent different feedstock tree species, production temperature regimes, and placements in the forest, i.e. above ground, in the humus layer or in contact with the mineral subsoil. The types of charcoal had different initial C concentrations mainly depending on their production temperature. Nevertheless, all types of charcoal at all placements in the forest showed an initial drop in their C concentrations, which subsequently rose back to reach near initial values in part of the charcoal types. In part of the charcoal types, N concentrations decreased throughout the experiment, exhibiting considerable variation among feedstock species, production temperature regime, and placements in the forest. C/N ratios rose especially in charcoal made from wood of Scots pine (Pinus sylvestris L.), and charcoal that had been stored in contact with the mineral subsoil showed the most rapid mass gain. Our results confirm the important influence of production temperature and feedstock type on the degradation of charcoal, but they also show that microbial activity and environmental conditions play significant roles in charcoal degradation and thus for the fate of pyrogenic carbon under natural conditions. 相似文献
94.
95.
GUO Yuan LIU Zong-zheng LIU Le MENG Fan-hua ZHANG Yan-ru ZHOU Huan-min LIU Chun-xia 《中国畜牧兽医》2016,43(11):3011-3018
In order to obtain the cloned sheep using bone marrow mesenchymal stem cells(BMSCs)as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son(RCP)was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment. 相似文献
96.
为研究PRRSV N蛋白的结构、功能以及N蛋白在病毒致病中的作用,以临床分离PRRSV毒株E11105为研究对象,采用Primer Premier 5.0设计一对特异性引物,经RT-PCR扩增出N基因片段,利用相关分子生物学软件对N基因序列进行分析;将N基因克隆连接到pColdⅠ原核表达载体上,经PCR、双酶切鉴定及序列测定后,得到重组质粒pColdⅠ-N,将pColdⅠ-N转入大肠埃希菌BL21(DE3)感受态细胞中,IPTG诱导表达后用SDS-PAGE蛋白电泳及Western blot验证分析。结果显示,分离株E11105N基因与北美洲型代表株VR-2332、欧洲型代表株Lelystad virus(LV)、中国2006年暴发的高致病性PRRSV代表株JXA1、中国代表株CH-1a的核苷酸序列同源性分别为93.3%、34.7%、99.2%、95.4%,氨基酸序列同源性分别为94.4%、15.3%、94.4%、99.2%;系统进化树显示,E11105株N基因与美洲型代表株VR-2332、中国高致病性JXA1毒株的亲缘关系较近;分离株E11105N基因所编码蛋白不存在跨膜区;二级结构主要以α-螺旋和无规则卷曲为主,分别占20.33%和63.41%;预测该蛋白可能存在5个较为明显的B细胞优势抗原表位。SDS-PAGE蛋白电泳结果表明,重组N蛋白主要存在于菌体沉淀中,分子质量约为16.7ku;Western blot结果显示,带His标签的重组表达蛋白能被His单克隆抗体识别,显色后条带约为16.7ku,与SDS-PAGE蛋白电泳的条带大小一致。 相似文献
97.
H3N2犬流感病毒(canine influenza virus, CIV)已在中国多地的犬群中流行,是禽流感跨宿主感染并形成新分支的近期案例。研究表明,PA-X基因与甲型流感病毒适应新宿主的能力相关,且其长度能够影响甲型流感病毒的复制及致病能力。为了解PA-X基因的长度变化对H3N2 CIV复制能力及致病力的影响,本研究利用H3N2 CIV的8质粒操作系统,拯救了三株重组H3N2 CIV毒株:PA-X基因表达大小为232个氨基酸多肽的亲本病毒CIV_PA-X_232;对PA编码区第191、192位氨基酸的密码子进行改造,PA-X基因不表达蛋白的重组病毒CIV_PA-X_Knock;对PA+1编码区第232位氨基酸进行突变,PA-X基因表达大小为252个氨基酸多肽的重组病毒CIV_PA-X_252。通过比较3株重组病毒的聚合酶活性,在MDCK细胞中的复制效率及对小鼠致病性的差异,来评价表达不同长度的PA-X基因对H3N2 CIV的影响。结果显示,CIV_PA-X_252和CIV_PA-X_Knock的聚合酶活性显著(P<0.05)高于CIV_PA-X_232,且CIV_PA-X_... 相似文献
98.
Du Yang Xin Mingdao Huang Shaoming 《保鲜与加工》1995,(5):50-56
A new type of three dimenstional inner microfin tube is devoloped. Under four different pressures and in the range of condensing mass velocities from 33 to 153 kg/m2 . s,and by the experimental study of the three kinds of tubes with different microfin parameters, the performances of the condensation heat transfer for R11 in the horizontal microfin tubes and obtained. The experiments show that the inner microfin tube is a hopeful and nighty effective condensing heat transfer enhancement tube. 相似文献
99.
A theoretical model is developed for the dropwise condensation heat transfer on the horizontal circular surface with radial gradient surface energy based on the heat transfer model of individual condensate drop and the size distribution model of condensate drop on homogeneous condensation surface.The effect of variation of contact angle on the gradient surface on condensation heat transfer and condensate drop size distribution is taken account of in this model.The theoretical calculation method was obtained to predict the dropwise condensation heat transfer coefficient on the horizontal circular surface with radial gradient surface energy under various wall subcooled temperature,contact angle profile on wall surface,and working fluid.The effects of surface energy gradient,wall subcooled temperature,and thermophysical properties of condensate on the condensation heat transfer are discussed respectively.The calculation results show that the condensation heat transfer coefficient increases slightly with the increase of wall subcooled temperature.As latent heat and surface tension increasing,the condensation heat transfer coefficient increases.A larger surface energy gradient induces a larger condensation heat transfer coefficient. 相似文献
100.
The authors conduct the experimental study and optimal computing for the condenser of automobile air conditioner. The especially the properties of heat transfer and flow at the side of air when using shutter fin. The authors the influence of several combinations of fin's geometry parameter to heat transfer and flow resistance. The maximal heat transfer is used as a criterion relation to select the fin's geometry parameters in the same facing air area of condenser. Furthermore, a program is designed to conduct optimal computing, and the computing results are basically consistent with experimental results. It shows that the optimal method is reasonable and the results can be applied to engineering practice. 相似文献