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991.
流式细胞术在水生生物DNA含量和倍性分析中的应用 总被引:3,自引:0,他引:3
利用流式细胞仪对鲤鱼、银鲫、黄颡、牙鲆等淡水和海水鱼类进行了DNA含量测定或倍性分析。通过几百个样品的测定和分析,认为流式细胞仪在DNA相对含量测定和倍性分析上结果比较稳定一致,可以用于大量样品的测定分析。同时对待测样品的保存、处理及上样量等进行了一系列比较研究,找出最好的保存方法、最简便的样品处理方法和最少的上样量。流式细胞仪在水生生物的遗传育种、种群分化等方面有很重要的应用价值。 相似文献
992.
J.J. MA Z.R. XU Q.J. SHAO J.Z. XU SILAS S. O. HUNG W.L. HU L.Y. ZHUO 《Aquaculture Nutrition》2008,14(5):464-471
Effects of dietary l ‐carnitine were studied in juvenile black sea bream (Sparus macrocephalus). The semipurified basal diet [crude protein 450 g kg?1 dry matter (DM); crude lipid 126 g kg?1 DM] was formulated to choose white fishmeal as the protein source and fish oil plus corn oil (1 : 1) as the lipid source. Six diets (control + diets 1–5) containing 0.1, 0.12, 0.16, 0.24, 0.39 and 1.1 g of l ‐carnitine kg?1 diet were fed to triplicate groups of black sea bream (initial weight 13.10 ± 0.05 g) for 8 weeks. At the end of the feeding trial, growth performance, body composition and antioxidant status were determined. The results showed that relative growth rate (RGR) was significantly improved by the elevation of dietary l ‐carnitine level from 0.1 to 0.24 g kg?1, but decreased with further increment (P < 0.05). Lipid content decreased significantly (P < 0.05) in the dorsal muscle whereas increased (P < 0.05) in the liver with the addition of dietary l ‐carnitine. Dietary l ‐carnitine supplements elevated enzymatic antioxidants (superoxide dismutase, SOD; catalase, CAT; glutathione‐S‐transferase, GST) activities (P < 0.05) yet decreased the content of non‐enzymatic factor, total sulphydryl groups (TSH) (P < 0.05). In summary, the optimum dietary l ‐carnitine level was 0.284 g kg?1 diet by second‐polynomial regression analysis based on RGR (y = ?647.4x2 +367.97x + 234.55; R2 = 0.977, x = dietary l ‐carnitine levels, y = RGR), and dietary l ‐carnitine addition within the levels adopted in our study could depress lipid peroxidation in tissues of juvenile black sea bream. 相似文献
993.
Mercedes Fernandez-Moreno Alberto Arias-Perez Ruth Freire & Josefina Méndez 《Aquaculture Research》2008,39(5):474-481
Aequipecten opercularis (Queen scallop) and Mimachlamys (Chlamys) varia (Black scallop) are important natural resources occurring in Atlantic and Mediterranean coasts. To develop an optimal sustainable exploitation plan, it is important to study the genetic structure of the different populations. In this study, we used polymerase chain reaction‐restriction fragment length polymorphisms for the determination of the genetic variation and population structure of these two species in different localities. Ten composite haplotypes were generated for A. opercularis and 15 haplotypes for M. varia. Of these, six and four were unique respectively. The analysis of the distribution of the different haplotypes between the localities showed no clear evidence of subdivision in A. opercularis, while in M. varia the results indicated that the two localities analysed should be managed as separate stocks. 相似文献
994.
Saeedeh Rafatnezhad Bahram Falahatkar Mohammad H Tolouei Gilani 《Aquaculture Research》2008,39(14):1506-1513
The influence of stocking density (1, 2, 4, 6 and 8 kg m?2) was investigated on haematological parameters, growth and fin erosion of great sturgeon (Huso huso) juveniles for a period of 8 weeks. The mean weight of fish at the start of trial was 93.13±1.04 g. After 8 weeks of rearing, the mean weight was 362.4, 319.7, 267, 242.1 and 211.1 in densities 1–8 kg m?2 respectively. The results of this study showed that growth parameters, including condition factor, weight, weight gain, feed conversion ratio, specific growth rate and body weight increase, had a statistically significant difference among treatments (P<0.05). A significant difference (P<0.05) was observed in haematocrit, but the other haematological parameters, including red blood cells, white blood cells (WBC), haemoglobin concentration, mean corpuscular haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentration and differential WBC count, showed no significant effect with stocking density (P>0.05). At the end of the experiment, stocking density had no significant effect on plasma cortisol and glucose concentration. The fin length (dorsal, anal, ventral, pectoral and caudal) of fish was measured to calculate the fin index. According to this index, dorsal, anal, ventral and pectoral fins showed no significant difference among treatments (P>0.05), but the erosion of the caudal fin was significantly different between fish held on 6 and 8 kg m?2 (P<0.05). These results showed that rearing density has a major effect on the growth indices of H. huso. Unlike many other fish, great sturgeon exhibited lower stress responses to high stocking density. This indicates that they are more tolerable to rearing conditions in high stocking densities. With respect to the various effects that density causes on growth, fin erosion and physiological and haematological parameters, better understanding of these phenomena considering different levels of density could have a beneficial impact on many rearing steps of this species. 相似文献
995.
平邑甜茶后代双胚苗的倍性鉴定及核型分析 总被引:1,自引:0,他引:1
为了确定平邑甜茶双胚苗的倍性特征和核型特点,采用压片和流式细胞仪观测的方法对平邑甜茶后代中双胚苗进行染色体倍性观察和核型分析。结果表明:4株供试材料均为三倍体,2n=3x=51,染色体为小型,染色体长度比差异不大;核型组成由中部着丝点染色体(m)和亚中部着丝点染色体(sm)组成;株系8-1、8-2、12-1和12-2的核型公式分别为2n=3x=45m+6sm,2n=3x=48m+3sm,2n=3x=27m+24sm,2n=3x=33m+18sm;染色体相对长度和着丝点指数的变异范围分别为1.21%~2.68%和34.19%~42.37%,1.44%~2.63%和35.59%~44.08%,1.36%~3.02%和32.85%~42.18%,1.43%~2.87%和34.23%~44.02%;核型分别属于1B、1A、2B和1B类型,表现为株系之间的核型不完全相同。本研究为丰富无融合生殖型砧木的育种理论,获得新的苹果无融合生殖型砧木提供有益的借鉴。 相似文献
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999.
Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree 总被引:2,自引:0,他引:2
Ningbo Cui Taisheng Du Shaozhong Kang Fusheng Li Xiaotao Hu Mixia Wang Zhijun Li 《Agricultural Water Management》2009,96(11):1615-1622
To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (ΔL) at different growth stages and fruit stable carbon isotope discrimination (ΔF) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on ΔL at different growth stages and ΔF at fruit maturation stage. As compared with full irrigation, the average ΔL at different growth stages and ΔF at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. ΔL and ΔF among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the ΔL and WUEi (photosynthesis rate/transpiration rate, Pn/Tr), WUEn (photosynthesis rate/stomatal conductance of CO2, Pn/gs), WUEy (yield/crop water consumption, Y/ETc) and yield, or between the ΔF and WUEy and yield were found, respectively. There were significantly negative correlations of ΔL with WUEi, WUEn, WUEy and yield (P < 0.01) at the fruit maturation stage, or ΔL with WUEi and WUEn (P < 0.01) over whole growth stage, respectively. ΔF was negatively correlated with WUEy, WUEn and yield (P < 0.05), but positively correlated with ETc (P < 0.01) over the whole growth stage. Thus ΔL or ΔF can compare WUEn and WUEy, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation. 相似文献
1000.
Water use of spring wheat to raise water productivity 总被引:1,自引:0,他引:1
In semi-arid environments with a shortage of water resources and a risk of overexplotation of water supplies, spring wheat (Triticum aestivum L.) is a crop that can reduce water use and increase water productivity, because it takes advantage of spring rainfall and is harvested before the evaporative demands of summer. We carried out an experiment in 2003 at “Las Tiesas” farm, located between Barrax and Albacete (Central Spain), to improve accuracy in the estimation of wheat evapotranspiration (ETc) by using a weighing lysimeter. The measured seasonal ETc averages (5.63 mm day−1) measured in the lysimeter was 417 mm compared to the calculated ETc values (5.31 mm day−1) calculated with the standard FAO methodology of 393 mm. The evapotranspiration crop coefficient (Kc) derived from lysimetric measurements was Kc-mid: 1.20 and Kc-end: 0.15. The daily lysimeter Kc values were fit to the evolution linearly related to the green cover fraction (fc), which follows the crop development pattern. Seasonal soil evaporation was estimated as 135 mm and the basal crop coefficient approach was calculated in this study, Kcb which separates crop transpiration from soil evaporation (evaporation coefficient, Ke) was calculated and related to the green cover fraction (fc) and the Normalized Difference Vegetation Index (NDVI) obtained by field radiometry in case of wheat. The results obtained by this research will permit the reduction of water use and improvement of water productivity for wheat, which is of vital importance in areas of limited water resources. 相似文献