Artificially regenerating longleaf pine in canopy gaps: initial survival and growth during a year of drought

Nitrogen fertilization in the nursery, along with altering the configuration of forest gaps, may improve the reforestation success of longleaf pine seedlings. During the very droughty 1998 growing season in Florida and Georgia, survival was higher under the forest canopy than in small (0.10 ha, 36 m diameter) and large (1.6 ha, 144 m diameter) canopy gaps. In the large gaps, survival of containerized seedlings was higher along the edges, particularly the SW edge. Shade from adult trees and the nurse effect of shrubs increased survival, while grass competition reduced survival. During dry years part of the “exclusionary zone” along the edge of canopy gaps (SW sector) may serve as a “survival zone”, at least in the short term. A model using oval-shaped gaps oriented from NW to SE, with an area of 0.25 ha is proposed to maximize the survival and growth of artificially regenerated longleaf pine seedlings.     

Management Effects on Biomass and Foliar Nutritive Value of <Emphasis Type="Italic">Robinia pseudoacacia</Emphasis> and <Emphasis Type="Italic">Gleditsia triacanthos</Emphasis> f. <Emphasis Type="Italic">inermis</Emphasis> in Arkansas,USA

The browse potential of black locust (Robinia pseudoacacia L.) and thornless honey locust [Gleditsia triacanthos f. inermis (L.) Zabel] has not been adequately tested. Our objective was to determine effects of fertilization and pollarding on biomass and foliar nutritive value in separate studies of black locust and thornless honey locust in Arkansas, USA. Shoots were sampled monthly for two consecutive growing seasons in 2002 and 2003 to determine foliar, shoot, and total aboveground biomass, shoot basal diameter, and foliar nutritive value (crude protein and in vitro digestibility). Black locust yielded more foliar biomass when pollarded at 50 or 100 cm and fertilized with 600 kg P ha⁻¹, than at 5 cm with or without P, averaging 3.5 Mg dry matter ha⁻¹. Black locust foliar crude protein and in vitro dry matter digestibility ( ≤ 170 and 534 g kg⁻¹, respectively) decreased as leaves aged, but still met maintenance needs for beef cattle (Bos taurus L.). Thornless honey locust had little agronomic potential because of slow establishment, low foliar yield (330 kg ha⁻¹), and a 2% reversion to undesirable thorny phenotype. Black locust should be considered for livestock browse when drought induces semi-dormancy of herbaceous forages.     

硅酸盐菌剂在砀山酥梨上的试用研究

研究硅酸盐菌剂在砀山酥梨栽培上的应用 ,结果表明在试验条件下最佳施用量为 1kg/株     

枣授粉受精及胚胎发育研究

The processes about pollination, fertilization and embryo development of Chinese Date ‘Wu-he-zao‘ (ziziphus jujuba) were observed by means of lightmicrocope combining histochemical dyeing and fluorescent technique. The results were summarized as follows:The pollen grains germinated on the stigma at the 4th hour after pollination. On the 5th day after pollination,the primary endosperm nucleus formed, and the zygote formed on the 6th day. The double fertilization of Wu-he-zao was the type premitotic syngamy. The first division of the zygote took place after a dormancy for about 6 - 7 days. The proembryonal development of Wu-he-zao belonged to the Solanad type. The development of the endosperm belonged to the nuclear type.     

The effect of multi-nutrient fertilization on understory vegetation nutrient concentrations in inland Northwest conifer stands

This study looked at the effects that multi-nutrient fertilization had on understory vegetation nutrient concentrations at four conifer forested locations in the inland Northwest. Multi-nutrient fertilization of conifer stands cannot only enhance the overstory species in the inland Northwest but also the understory vegetation. Determination of nutrient concentration response to fertilization treatments can provide managers the ability to better manipulate their forests for grazing and wildlife habitat. We grouped the understory vegetation into three general life forms: forbs, grasses and grass-likes, and shrubs. Multi-nutrient fertilization had little effect on nitrogen concentration across all life forms. Potassium and sulfur generally increased in concentration. Micronutrients as a whole showed less variability in response to multi-nutrient fertilization. Boron, copper, molybdenum, and zinc generally showed increases in concentrations across all life forms. We were able to conduct analyses on a selected number of understory vegetation species. Individual species showed variability in nutrient concentration response to multi-nutrient fertilization. Wildlife habitat and grazing quality were both increased and decreased following multi-nutrient fertilization. Increases in nutrient concentrations will provide more nutritious vegetation to these animals and vice versa for decreases in concentrations.     

Influence of nursery soil amendments on water relations,root architectural development,and field performance of Douglas-fir transplants

This experiment evaluated the influence of manure, peat, and vermiculite incorporated at low and high rates (0.0118 and 0.0236 m³/m²) and under two soil moisture regimes on Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedling (1+0 for 1+1) xylem water potential (_xylem), whole-plant growth, root architectural development, and subsequent field performance under fertilized and non-fertilized conditions. Trends in soil moisture retention were observed (high manure > high peat > control) but there were no differences in _xylem. Root length in the wetter soil moisture experiment was initially (three months) greatest for seedlings in high vermiculite and least in high manure but there were no differences among treatments at lifting (eight months). Mean height was greatest for seedlings grown in vermiculite and peat (wetter nursery experiment) after two field seasons. Field fertilization (35 g/seedling) with controlled-release fertilizer in the planting hole stimulated height growth initially, but decreased height and diameter growth during the second growing season. Dramatic improvements associated with the use of nursery soil amendments were not realized, but the failure to identify negative effects, a potential reduction in disease incidence, and improvement of nursery soil physical and chemical properties may justify their use.     

提高杉木种子园种子产量及品质技术措施的研究

对湖南省东安县杉木无性系种子园施肥试验的结果表明：３、６、８月中旬每次每株各施尿素０．４ｋｇ、过磷酸钙０．３ｋｇ、氯化钾０．２ｋｇ为球花产量最高的施肥方案；施肥对当年的种子播种品质有影响，但不大；种子园大片母树不挂果的原因是土壤和树体中锰含量过高而树体中的硼元素缺乏，对此提出对土壤施石灰和对母树针叶喷Ｂ肥的改良措施；３—８月是杉木种子园施Ｎ、Ｐ．Ｋ肥最适宜时期；确定８月是杉木种子园母树营养诊断进行针叶分析的最佳采样时期，并制定出杉木种子园母树的针叶中全Ｎ含量为０．８％、全Ｐ含量为０．１２％、全Ｋ含量为０．７％是其临界值；针叶全Ｎ含量０．８—１．２％、全Ｋ含量０．７－０．９％是其最适浓度范围；中耕松土对种子园种子产量影响不明显。     

作物病害智能诊断与处方推荐技术研究进展

由“植物诊所”形成的电子病历为作物病害处方推荐提供了新的思路。如何高效地挖掘电子病历数据并辅助作物病害处方推荐,目前还是亟待解决的研究热点问题。在总结和整理现有国内外研究文献的基础上,对基于显微图像的作物病害病菌孢子识别、基于光谱的作物病害诊断、基于电子病历的作物病害处方推荐等作物病害诊断与处方推荐关键技术进行了系统分析与讨论。综述结果表明,围绕作物病害病菌侵染过程,以智能化处方推荐需求为导向,开展基于电子病历数据挖掘的作物病害处方推荐研究,将成为一个研究重点。针对作物病害处方推荐过程中,存在由于作物病害致病机理复杂、作物品种及病害种类多、病害病症动态变化且特征多等特点和难点,研究基于电子病历数据挖掘的作物病害致病机理解析、诊断推理、智能化处方推荐及其应用策略,将是研究的重大方向;探索基于知识图谱分析、大数据挖掘和机器学习算法推理等关键技术的作物病害电子病历数据挖掘分析研究,从区域宏观视角可视化解析作物病害致病机理及其与特征间的关联关系,面向实际应用场景实现基于诊断推理的单一作物病害处方推荐、基于语义匹配的多种作物多种病害处方推荐,具有更大的实际意义。     

基于江苏茶园施肥及空间分布特性分析江苏茶园可持续发展

【目的】茶树是一种多年生作物,现已成为我国乡村致富的重要产业,为了掌握江苏茶园土壤养分现状及施肥方式对茶园养分环境的影响分析。【方法】通过对江苏省主产茶128个茶园施肥方式进行调研,测定其有机质、pH值、碱解氮、速效磷、全钾以及钙、镁等微量元素及部分重金属物质含量,同时对经纬度和不同茶园营养元素之间的相关性进行分析。【结果】江苏茶园pH值介于3.82～6.14之间,有机质含量介于11.10～29.4g/kg之间,碱解氮含量介于42.23～119.45mg/kg之间;速效磷含量介于13.75～289.71mg/kg之间;全钾含量介于53.61～79.35g/kg之间;有机质含量与取样点经纬度均存在显著相关关系(相关系数分别为0.7430和-6910),同时土壤全钾含量与取样点经度均存在显著负相关关系(r=-0.820),与取样点纬度存在显著正相关关系(r=0.6992),有机质含量与碱解氮和速效磷含量显著正相关(r=0.8319和r=0.6681),与速效钾含量间存在显著负相关关系(r=-0.9093);土壤pH值和镍元素之间存在极显著正相关关系(r=0.9049)。【结论】尿素的大量施用是导致江苏茶园土壤酸化严重的重要原因,总体而言,江苏茶园土壤总体肥力较好,部分茶园存在酸化现象,茶园有机质含量越高,碱解氮和有效磷含量越高,有效钾含量越低。施肥习惯应结合有机肥(菜籽饼)与尿素相结合的方式,并注意土壤改良剂的应用和尿素的施用,更有利于茶园营养物质的可持续利用和高质量发展。     

Prematurational Culture with 3-Isobutyl-1-methylxanthine Synchronizes Meiotic Progression of the Germinal Vesicle Stage and Improves Nuclear Maturation and Embryonic Development in In Vitro-grown Bovine Oocytes

The objective of this study was to clarify the effects of prematurational culture (pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95 μm in diameter) derived from early antral follicles (0.5–1 mm in diameter) were cultured for 12 days for in vitro growth (IVG). IVG oocytes with a normal appearance were subjected to examinations of diameter and chromatin structure in the germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about 115 μm. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2, the fertilizability and developmental competence of IVG oocytes were examined. Regardless of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but were lower than that of in vivo-grown oocytes (88.0%). Cleavage and blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with pre-IVM was lower than that of in vivo-grown oocytes. In conclusion, pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes, probably due to promotion of their chromatin transition and synchronization of meiotic progression.