转Bt基因棉对棉铃虫抗性的时空动态

在室内研究了转Ｂｔ基因棉对棉铃虫抗性的时空动态及对棉铃虫幼虫取食行为的影响,结果表明,整个棉花生长期,棉花不同器官随着时间的变化其抗性有较大的差异,如７月以后,棉叶对棉铃虫幼虫的抗性明显下降,由６月下旬的１００％下降到８月初的６２．５％,然后抗性又逐渐回升,但仍为下降的趋势;从空间抗性来看,营养器官的抗性高于繁殖器官,接虫２４ｈ后,棉铃虫幼虫在转基因棉繁殖器官上分布数量较多;转基因棉对棉铃虫幼虫取食行为有较大的影响,和常规棉相比,在转基因棉上取食时间减少５７．６％,吐丝下垂、爬行和静息的时间分别延长３９６．１％、２２．４％和４．０％。     

Feeding responses of juvenile shrimp Litopenaeus vannamei (Boone) fed at different frequencies under laboratory conditions

In shrimp farms, food partitioning during the course of the day is contradictory, ethology studies may help in determining the proper frequency. Litopenaeus vannamei juveniles were placed in 30 L aquariums (41 m⁻²), exposed to a 12:12 h light–dark cycle. Feeding was provided at 10% of biomass three, four and seven times per day, with observations made in 15 min h⁻¹ windows, initiated after the ration was offered. Latency to access the feeding tray and to start eating was recorded using instantaneous focal sampling and digestive tract filling (DTF) by the continuous focal method. Weight gain was recorded at the end of the experiment. We performed seven repetitions, with 28 individuals observed for 33 days for each treatment (490 h of observation). The three-times offering induced less latency for accessing the tray and for ingesting the feed as well as a higher DTF when compared with the other frequencies. Animals fed three and four times had similar weight gains, and were greater than those fed seven times. Our results indicate that a more spaced food offering stimulates the search for and ingestion of feed. As compared with other frequencies, the three-times-per-day option assumes lower labour costs and a more efficient use of the feed.     

Antipredator behaviour of naïve Arctic charr young in the presence of predator odours and conspecific alarm cues

Abstract –  We investigated how Arctic charr young respond behaviourally to olfactory cues from skin-damaged conspecifics, charr-fed pikeperch, and the combination of food-deprived pikeperch and skin-damaged conspecific cues in a two-channel Y-maze fluviarium test arena. Significant antipredator responses were found to skin-damaged conspecifics in three behavioural traits, to charr-fed pikeperch in two traits and to the combination of pikeperch and skin-damaged conspecific cues in all six behaviours investigated. The combination of predator and conspecific cues significantly increased spatial avoidance and cautiousness to approach the source of scent compared with odours from charr-fed predators. The results suggest that damage-released alarm cues exist in charr, and they strengthen the antipredator responses in conspecifics. As the charr used different behaviours and levels of response in the presence of different cues, they seem to have sophisticated skills to distinguish between different odour combinations and be able to adjust their behaviour according to the current predation risk level.     

Transpositional feeding rhythm of loach <Emphasis Type="Italic">Misgurnus anguillicaudatus</Emphasis> from larvae to juveniles and its ontogenesis under artificial rearing conditions

The diel feeding rhythm and ontogenesis during early life stage of loach Misgurnus anguillicaudatus were investigated under experimental conditions (light: L 06:00–18:00, D 18:00–06:00 h). Morphological and behavioral developments of loach from newly hatched to 40 days after hatching were observed. Larvae were able to prey on daphnia 3–4 days after hatching at 23 ± 0.5°C. As the larvae grew, they showed an increasing feeding capacity and a distinct feeding rhythm. Feeding intensity and incidence for day-4 larvae were highest at 10:00 and 16:00 h. The highest levels of feeding intensity for day-12 larvae occurred at 08:00, 12:00, and 18:00 h as did feeding incidence. By day 20, when the larvae metamorphosed, the highest levels of feeding intensity occurred at 06:00, 18:00, and 24:00 h and were concurrent with the highest feeding incidence. After metamorphosis, feeding capacity had again increased considerably and, in contrast to the earlier stages before day 20, feeding intensity for day-30 juveniles peaked at 05:00 and 20:00 h, about 1–2 h after the maximum feeding incidence. The feeding rhythm of loach juveniles at day 40 was almost the same as the day-30 juveniles. The estimated maximum daily feeding rates were 43.1%, 33.4%, 19.0%, 12.8%, and 5.8% of body weight on days 4, 12, 20, 30, and 40, respectively. Thus, loach was found to have different feeding rhythms in the pre- and post-metamorphosis stages, with the highest feeding activity in daytime during the larval planktonic stage before metamorphosis, and intensely nocturnal feeding behavior during the juvenile benthic stage after metamorphosis.     

Effects of dietary macronutrient level and feeding frequency on growth and body composition of juvenile rockfish (Sebastes schlegeli)

An 8-week feeding experiment was conducted to investigate the effects of different dietary macronutrient level and feeding frequency on the growth, feed utilization, and body composition of juvenile rockfish. Triplicate groups of fish (body weight of 4.1 g) were fed the experimental diets containing either high levels of carbohydrate (HC, 35%), lipid (HL, 13%), or protein (HP, 55%) at different feeding frequencies (twice daily, once daily, and once every 2 days). Weight gain was affected by feeding frequency but not by dietary composition. Weight gain of fish fed the diets once every 2 days was significantly (P < 0.05) lower than that of other groups. Daily feed intake and energy intake were affected by both dietary composition and feeding frequency. Daily feed intake of fish fed the HC diet was significantly (P < 0.05) higher than that of fish fed the HL and HP diets at the same feeding frequency. Feed efficiency and protein efficiency ratio were affected by both dietary composition and feeding frequency and decreased with increasing feeding frequency in the same dietary composition. Feed efficiency and protein efficiency ratio of fish fed the HC diet were significantly (P < 0.05) lower than those of fish fed the HL diet at the same feeding frequency. Whole-body lipid content of fish fed the HL diet was significantly (P < 0.05) higher than that of fish fed the HC and HP diets at the same feeding frequency. These results indicate that an increase of dietary lipid level compared with dietary carbohydrate level may have the advantage of a protein-sparing effect at same feeding frequency, and a once-daily feeding regime is more effective than twice daily or one feeding every 2 days to improve growth performance of juvenile rockfish grown from 4 to 21 g.     

Evaluation of codends with sorting grids, exit windows, and diamond meshes: Size selection and fish behaviour

This paper presents the results of an experiment aimed at improving the size selection of cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) in the Nordic bottom trawl fishery. Three systems simulating commercial conditions were tested: a 135 mm diamond-mesh codend fitted with a 55 mm sorting grid (Sort-V); a 135 mm diamond-mesh codend fitted with two lateral exit windows; and a codend built entirely of 155 mm diamond mesh. The selectivity curves showed similar selection ranges (SR) for the three systems. For cod, the mean SRs were around 8 cm, while for haddock, they were around 6 cm. All the estimated l₅₀ values were far above the minimum landing sizes (MLS). For cod, the mean l₅₀s were 56.1, 53.9, and 60.7 cm for the sorting grid, exit windows, and diamond-mesh configurations, respectively, while for haddock, they were 50.2, 50.6, and 49.9 cm, respectively. Underwater observations revealed that most of the fish escaped from the grid and exit-window codends as soon as they reached the vicinity of the sorting device. In contrast, fish remained inside the diamond-mesh codend for a longer time, and consequently were more exposed to physical damage before escape. In addition, many fish escaped from this codend during haul back and these fish were not likely to survive due to the rapid changes in pressure. Thus, the selectivity results obtained with this type of codend should be interpreted carefully.     

山西四种鸮形目鸟类生态习性的初步研究

本研究于山西吴城地区对4种鸮形目鸟类的生态习性进行了研究,旨在积累该地区鸮形目鸟类的生态学基础资料。利用样线法记录迁徙时间,并收集繁殖季栖息地、巢卵特征的数据资料;利用食团内容物分析法分析食性。结果表明不同年份红角鸮、长耳鸮、短耳鸮的迁徙具有相似特点,均是北迁持续期时间短、间隔时间长,南迁持续期时间长,间隔时间短。红角鸮与纵纹腹小鸮在巢址选取、育雏行为等方面存在差异,营巢地更接近居民区的纵纹腹小鸮的繁殖成功率相对较高。4种鸮形目鸟类均以小型哺乳类和鸟类为主要食物,此外也取食少量昆虫和部分家畜、家禽。不同物种之间需求的重叠会导致种间竞争的增加,考虑到鸮形目鸟类在生态系统中的重要作用及其偏低的种群数量,应对其加以关注并实施保护。     

消费者生鲜水果线上购买迁徙行为及其影响因素

为探索消费者生鲜水果线上购买迁徙行为的影响因素和机制路径,本研究基于PPM理论,构建结构方程模型分析了消费者生鲜水果线上购买迁徙行为及其影响因素,并运用多群组分析不同消费者行为影响因素的差异性。结果表明：1）价格、信息、服务不满意构成正向影响消费者生鲜水果线上购买迁徙行为的推力因素;感知有用性、搜索、消费体验感构成正向影响迁徙行为的拉力因素;程序转移成本构成负向影响迁徙行为的锚定因素。2）锚定因素负向调节推力因素对迁徙行为的作用效果。3）性别、年龄、学历水平三个人口统计特征在不同假设路径中的影响较为显著。其中女性、30岁及以下、学历水平大专以上的群体更愿意在线上渠道购买生鲜水果。结论丰富了生鲜水果领域消费者迁徙行为的研究成果,并为生鲜电商可持续发展提供了启示。     

饲料中动物源性成分显微近红外光谱检测分析

探讨了应用显微近红外光谱分析技术检测饲料中动物源性成分的可行性.利用12个动物源性成分饲料样品和14个植物源性成分饲料样品建立偏最小二乘定标模型,数学预处理方法为导数+多元散射校正,利用平均光谱来优化模型实现数据压缩,模型的决定系数、交互验证标准差分别为0.969、0.090.4个配合饲料样品作为外部验证未出现误判.结果表明显微近红外光谱分析技术可用于检测饲料中动物源性成分.     

Carbon and nitrogen budgets of nematodes in arable soil

Summary The amounts of C and N that pass through the nematode biomass in four cropping systems, barley without and with N fertilization, grass ley and lucerne, has been estimated. The nematodes were sampled at the field site of a Swedish integrated research project Ecology of Arable Land: The Role of Organisms in Nitrogen Cycling. The nematode biomass was lower (200 mg dry weight m^–2) in the annual (barley) than in the perennial (grass and lucerne, 350 mg dry weight m^–2) crops. For respiration, the nematodes used 4–71 O₂m^–2 year^–1 corresponding to C liberation of 1.3%–2.0% of the carbon input to the soil. A higher relative contribution by bacterial-feeding nematodes to the C and N fluxes and a higher turnover rate of the nematode biomass is an indication of more rapid nutrient circulation in the annual than in the perennial cropping systems.