春花生超高产栽培技术模式研究与应用

采取正交旋转回归设计,以播种期、种植密度、施肥量为试验因子,以9000kg/hm2为产量目标函数,进行春花生超高产栽培模式集成研究。结果表明,在鲁西南平原地区,超高产春花生以种植密度和N、P、K施肥量为主要限制因素,播种期为次要限制因素。明确了春花生单产达到9000kg/hm2以上的最佳种植密度、播种期和合理的N、P2O5、K2O施用量为主要指标的栽培技术模式,增产效果显著。     

Quantitative trait loci for fruit size and flowering time-related traits under domestication and diversifying selection in cucumber (Cucumis sativus)

In cucumber, the genetic basis of traits under domestication and/or diversifying selection is not well understood. Here, we reported QTL mapping for flowering time and fruit size-related traits with segregating populations derived from a cultivated × wild cross. Phenotypic data of flowering time (FT), fruit size (FS), fruit number (FN) and fruit weight per plant (FW) were collected in multiple environments. QTL analysis identified 19 QTL for these traits. We found that the major-effect QTL FT1.1 played an important role in regulating flowering time in cultivated cucumber, whereas the minor-effect QTL FT6.3 contributed to photoperiod sensitive flowering time during domestication. Two novel consensus FS QTL, FS1.4 and FS2.3, seem to be the targets of selection during breeding for the US processing cucumber. All other FS QTL were co-localized with previously detected QTL using populations derived from cultivated cucumbers, suggesting that they were under selection during both initial domestication and subsequent improvement. Results from this study also suggested that the wild cucumber is a useful resource for capturing positive transgressive segregation and novel alleles that could be explored in cucumber breeding.     

High night-time temperature during flowering and pod filling affects flower opening,yield and seed fatty acid composition in canola

Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod-filling stages. Although the impact of high day-time temperature stress on yield and quality has been documented in canola, similar information under high night-time temperature (HNT) stress is not available. Using six hybrids and four open-pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post-flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open-pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open-pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future.     

基于状态变换法的车辆悬架系统时滞反馈控制

为了提高车辆行驶平顺性和稳定性,研究悬架系统中时滞补偿控制效果,本文以赛欧轿车悬架系统为基础,建立2自由度车辆半主动悬架系统模型,设计了时滞反馈控制器,采用理论与试验相结合的方法对系统时滞反馈控制特性进行研究。首先建立含时滞的悬架系统动力学方程,采用常微分理论和多项式判别方法分析系统稳定性,并通过时域与频域仿真对结果进行验证。研究表明:采用传统二次型最优控制律对含时滞的悬架系统进行控制,当系统控制时滞较大时,系统定性特性可能会发生改变,甚至会失稳发散。为保证系统的稳定性,采用状态变换方法设计时滞反馈最优控制律,仿真表明采用该控制律不仅可以保证系统稳定性,系统的减振特性亦有改善。最后搭建了悬架时滞反馈控制平台,基于时域辨识方法测得系统固有时滞为0.065 s,通过对相同工况下仿真结果与试验结果进行对比,发现两者具有较好的一致性,误差在15%以内,满足业内使用要求,表明研究可信,结果可为主动/半主动车辆悬架控制器实际设计应用提供参考。     

Serum amyloid A (SAA) mRNA expression in chicken and quails in response to bacterial stress

Monitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red chicken (RIR) and Japanese quails using real time PCR analysis in response to inactivated Salmonella gallinarum culture. The results showed that expression of SAA gene was approximately 17–33 folds higher in case of birds administered with bacterial culture when compared to un-inoculated controls and expression was higher and quicker in case of quails than RIR chicken. The SAA genes from chicken and quail were cloned and upon sequence analysis it was observed that deduced amino acid sequence of SAA from chicken and quails were having approximately seven percent variation which might have significance in function of this protein in these species.     

关税和补贴对国企国有股份最优占比的影响——基于混合寡占理论分析

通过建立理论模型，并用2014年湖南省5县区20个城乡社区的调查数据，从家务劳动时间的视角分析湖南劳动力市场的性别工资差异及其影响因素。结果发现，家务劳动时间是影响湖南劳动力市场性别工资差异的重要因素，同时其他影响因素还有月工资收入、受教育程度、行业等，但职务职称对性别工资差异的影响存在不确定性。因此，政府应大力发展家庭服务业，建立健全覆盖城乡的家庭服务体系，基本满足家庭的服务需求，使女性从家务劳动中解脱出来，同时应通过政策倾斜确保女性培训比例，增强就业竞争实力；规范劳动合同，维护私有及民营企业女性从业者在劳动就业、社会保障等方面的合法权益。     

Simulation of future dissolved oxygen distribution in pond culture based on sliding window-temporal convolutional network and trend surface analysis

Dissolved oxygen (DO) is a key ecological factor to measure the quality of water in the aquaculture. As the pond water body is affected by the breeding environment, the spatial distribution of DO shows a certain law in the entire pond. Therefore, to simulate the distribution of DO in aquaculture waters and grasp the temporal and spatial variation of DO is the key to achieving precise regulation of DO. For this purpose, this paper proposed a method for simulating the temporal and spatial distribution of DO in pond culture based on a sliding window-temporal convolutional network together with trend surface analysis (SW-TCN-TSA). This paper first utilized SW to construct DO data sets with different prediction durations, and then used the improved TCN model to realize one-dimensional time series prediction for DO at single monitoring point. Based on the prediction results of DO, a TSA method was performed on the predicted values of DO at the extreme moments of all discrete monitoring points, so as to realize the simulation of the temporal and spatial distribution of DO in the pond. Experimental results show that the SW-TCN model has better prediction performance for one-dimensional time series prediction of DO. Compared with traditional deep networks, such as CNN, GRU, LSTM, CNN-GRU and CNN-LSTM, the values of evaluation indicators (MSE, MAE and RMSE) have been greatly improved. In the process of trend surface fitting, all fitting R² of DO at different water depths are higher than 0.9, indicating that the TSA can accurately reflect the temporal and spatial distribution of DO. This method can provide a basis for the prediction and early warning of DO in the three-dimensional space of the pond and has high practicability in aquaculture.     

自动施肥系统开发与性能测试

目的 开发一种适应于以固态水溶肥为原料的自动施肥系统,测试分析自动施肥系统性能。方法 主控机采用ARM9电路控制模块可实现对轮灌编组、预搅拌时长、施肥开始与结束时间、施肥持续时长、施肥量等参数的设置;选择以蠕动泵为注肥装置,通过变频器控制注肥泵电机功率的方式控制注肥速率,控制施肥量。对装置核心部件搅拌器额定功率、计量方式、溶肥搅拌参数、排肥速度及固液相比例等主要参数等进行设计与测试。结果 电感脉冲计量方式标准误差最大值1.26%,误差小、性价比好,确定其为本装置采用的计量方式;搅拌器以1.5 Kw额定功率、38 r/min转速搅拌、肥液浓度在1.1~1.3 g/mL、预搅拌时间30 min时,罐内各液位输出肥液浓度值差异不显著(P< 0.05),达到对肥料浓度均匀性的设计要求。结论 将施肥开始前的预搅拌时间设为30 min、搅拌转速设为38 r/min、肥液浓度不高于1.3 g/mL,输出肥液浓度有较好的均匀性,实现精准施肥。     

Impact of High Night‐Time and High Daytime Temperature Stress on Winter Wheat

High temperature is a major environmental factor that limits wheat (Triticum aestivum L.) productivity. Climate models predict greater increases in night‐time temperature than in daytime temperature. The objective of this research was to compare the effects of high daytime and high night‐time temperatures during anthesis on physiological (chlorophyll fluorescence, chlorophyll concentration, leaf level photosynthesis, and membrane damage), biochemical (reactive oxygen species (ROS) concentration and antioxidant capacity in leaves), growth and yield traits of wheat genotypes. Winter wheat genotypes (Ventnor and Karl 92) were grown at optimum temperatures (25/15 °C, maximum/minimum) until the onset of anthesis. Thereafter, plants were exposed to high night‐time (HN, 25/24 °C), high daytime (HD, 35/15 °C), high daytime and night‐time (HDN, 35/24 °C) or optimum temperatures for 7 days. Compared with optimum temperature, HN, HD and HDN increased ROS concentration and membrane damage and decreased antioxidant capacity, photochemical efficiency, leaf level photosynthesis, seed set, grain number and grain yield per spike. Impact of HN and HD was similar on all traits. Greater impact on seed set, grain number and grain yield per spike was observed at HDN compared with HN and HD. These results suggest that HN and HD during anthesis cause damage of a similar magnitude to winter wheat.