渭北地区富士苹果不同砧穗组合幼树内源激素含量与早花性和易成形性关系的研究

以3~4年生5个不同砧穗组合富士苹果幼树为材料,研究植物内源激素含量与早花性和易成形性间的关系。结果表明:乔化砧组合树体高大、生长旺盛,短枝比例和花序数量均显著低于矮化砧组合。矮化砧木组合中,M26组合虽然短枝比例很高,但单株成花量低,树体长势弱;SH16组合次之;T337组合长势中庸、单株成花量大。一年中,春、秋梢开始生长期,幼叶IAA、ZR含量出现高峰,根系第2次生长高峰期,细根IAA、ZR、GA_3含量出现高峰。幼叶ZR和细根IAA含量呈负相关,IAA、GA_3分别在地上部和地下部之间存在正相关。5月5日,幼叶IAA和ZR含量分别与当年生枝量呈正相关;5月20日,ZR、ZR/GA_3分别与短枝比例呈正相关,GA_3与花序数量呈负相关;根系第2次生长高峰期,苹果幼树开花相关指标分别与细根中的IAA、ZR、GA_3、IAA/ZR呈正相关,说明内源激素在地上部成花与地下部根系生长过程中具有重要调节作用。渭北地区,T337自根砧木富士苹果组合体现了早花性和树体易成形性的统一。     

苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析

以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。     

苹果‘长富2号’开花调控转录因子基因MdIDD7的克隆及表达分析

以‘长富2号’苹果为试验材料,采用RT-PCR的方法,从其芽中克隆得到INDETERMINATE DOMAIN(IDD)转录因子基因MdIDD7,其开放阅读框长度为1 626 bp,编码541个氨基酸。序列比对和结构域分析表明,该转录因子含有1个核定位信号和4个高度保守的锌指蛋白结构域。系统进化树分析表明,MdIDD7与白梨(Pyrus×bretschneideri,XP_009364602.1)、桃(Prunus persica,XP_007225628.1)和梅(Prunus mume,XP_008220893.1)聚在一起。实时荧光定量PCR表明,MdIDD7在‘长富2号’不同组织(茎、叶、花、果和芽)中均有表达,其中芽的表达量最高。花后40~60 d,MdIDD7在易成花品种‘烟富6号’芽中表达量显著高于难成花品种‘长富2号’。"小年"树顶芽组织中MdIDD7的表达量显著高于"大年"树。在花芽诱导前期,外源GA处理诱导MdIDD7下调表达,而蔗糖处理诱导其上调表达,说明MdIDD7响应激素和糖信号,促进苹果成花。     

硼对平邑甜茶幼苗硝态氮吸收、利用及分配特性的影响

以平邑甜茶幼苗为试材,运用~(15)N同位素示踪和非损伤微测技术,研究了不同供硼(硼酸0、1.0、2.0、3.0、4.0、5.0和6.0 mg·L~(-1))水平对平邑甜茶根系生长及氮素吸收、利用和分配特性的影响。结果表明,3.0 mg·L~(-1)硼酸处理的幼苗根系活力及根系形态指标显著高于其他处理,幼苗的全氮量及~(15)N吸收量增幅最大,分别比对照提高了19.4%和75.0%。随供硼水平的增加,植株氮素利用率呈现先增高后降低的趋势,在3.0 mg·L~(-1)硼酸处理时最大,为14.8%,是对照的1.8倍。施硼处理对幼苗的~(15)N分配率有一定的影响,3.0 mg·L~(-1)硼酸处理的根系~(15)N分配率达到最大,且显著高于对照。非损伤微测结果显示,3.0 mg·L~(-1)硼酸处理时,平邑甜茶根系对NO_3~-有强烈吸收且内流速度达到最大,在缺硼和高硼(硼酸0和6.0 mg·L~(-1))处理时有明显外排趋势。因此,3.0 mg·L~(-1)硼酸处理最有利于平邑甜茶根系的生长、根系活力的提高和氮素的吸收利用,而低硼和过量供硼均会抑制根系生长及氮素的吸收利用。     

苹果分子标记及辅助育种研究进展

从基于集群分离分析法、杂交群体、全基因组关联分析和功能基因分子标记4个方面对苹果相关的分子标记及其在辅助育种上的应用进行了综述,为苹果分子标记辅助育种和揭示重要性状的遗传提供依据。     

Investigation and genetic mapping of a Glomerella leaf spot resistance locus in apple

Apple Glomerella leaf spot (GLS) is a severe fungal disease that damages apple leaves during the summer in China. Breeding new apple varieties that are resistant to the disease is considered the best way of controlling GLS. Fine mapping and tightly linked marker are critically essential for the preselection of resistant seedlings. In this study, a population of 207 F₁ individuals derived from a cross between ‘Golden Delicious’ and ‘Fuji’ was used to construct a fine simple sequence repeat (SSR)‐based genetic linkage map. The position of R_gls, a locus responsible for resistance to GLS, was identified on apple linkage group (LG) 15 using SSR markers CH05g05 and CH01d08, which was adapted from a published set of 300 SSR markers that were developed using the bulked segregant analysis (BSA) method. These two SSR markers flanked the gene, and its recombination rate was 8.7% and 23.2%, respectively. A total of 276 newly developed SSR markers around the target region and designed from the genome apple assembly contig of LG15 were screened. Only nine of these were determined to be linked to the R_gls locus. Thus, a total of 11 SSR markers were in linkage with R_gls, and mapped at distances ranging from 0.5 to 33.8 cM. The closest marker to the R_gls locus was S0405127, which showed a genetic distance of approximately 0.5 cM. The first mapping of the gene R_gls was constructed, and the locations of the 11 effective primers in the ‘Golden Delicious’ apple genome sequence were anchored. This result facilitates better understanding of the molecular mechanisms underlying the trait of resistance to GLS and could be used in improving the breeding efficiency of GLS‐resistant apple varieties.     

苹果种植区的土壤养分含量情况研究—以白水县为例

为了稳定和提高苹果产量,通过对2013 年渭南白水县500 个点农业土壤区进行分析,并对土壤pH、全氮、有机质、碱解氮、速效钾、有效磷、有效铁和有效锰、锌、铜进行有效的对比研究。利用Microsoft Excel 2010 软件进行数据整理统计。结果发现：白水县苹果生长的土壤酸碱度为中性偏碱,并且土壤中的全氮和有机质的含量丰富,速效钾较丰富;有效锌、有效磷含量适中;有效铜含量高,有效铁、碱解氮、有效锰含量低。     

基于高光谱成像的苹果品种快速鉴别

以"乔纳金"苹果,"红富士"苹果和"秦冠"苹果共90个试验样本为试材分别采集865~1 711 nm的近红外波段高光谱图像,选取苹果图像感兴趣区域(ROI),以分辨率2.8 nm提取其平均反射光谱数据,分别利用K近邻法(KNN)和径向基核函数支持向量机(RBF-SVM)进行品种判别,5折交叉检验。结果表明,3种苹果的近红外高光谱图像均在波长941~1 602 nm之间变得清晰,该区域200个波段下的平均反射光谱数据经KNN法中的10种距离算法评判,当K取值3和5时,切比雪夫距离、欧几里得距离和明可夫斯基距离3种距离算法的识别正确率均达到100%;SVM-RBF核函数模型中,γ取值为2-8~1的范围内识别正确率均在92%以上,当γ取值2-5,C取值为16和32时,识别正确率最高,为96.67%。故利用近红外高光谱图像技术结合KNN计算对苹果品种进行快速鉴别是优异和可靠的方案。     

Characterization and pathogenicity of six Cytospora strains causing stem canker of wild apple in the Tianshan Forest,China

Cytospora species are capable of causing destructive cankers of stems belonging to a wide range of woody plant species. In severe cases, cankers may lead to dieback of twigs and branches. Little is known about the Cytospora species causing canker disease of wild apple (Malus sieversii) trees in the Wild Fruit Forest Reserve in Tianshan Forest, Xinjiang Uygur Autonomous Region, China. In this study, six Cytospora isolates belonging to two species were isolated from cankerous lesions of wild apple twigs. Based on multi‐locus phylogenetic analysis using three DNA markers (ITS, tef1‐α and tub2) and morphological characterization, these isolates were identified as Cytospora mali and Cytospora parasitica. Temperature trials (15, 20, 25 and 30°C) showed that the optimal growth temperature for six isolates was 25°C. At a variety of temperatures, C. mali isolates tended to grow faster than isolates of C. parasitica, with the C. mali isolate, EGI1 performing better than others with regard to growth rate. Morphological observations showed that these species exhibited a single locule without conceptacles, and the conidia length was 3–5 μm. In vitro inoculation trials of twigs and leaves of M. sieversii seedlings revealed that the C. mali isolates were highly virulent phytopathogenic fungi, whereas the C. parasitica isolates were less virulent. The isolate EGI1 was the most virulent isolate among the six isolates. This paper presents the first report of pathogenic Cytospora spp. of the M. sieversii Tianshan Wild Fruit Forest Reserve of Yili, Xinjiang in China. It will aid in the understanding of how apple tree cankers are induced and provide disease management guidelines for M. sieversii forest conservation.     

山楂海棠RAPD亲缘关系研究

应用RAPD分子标记技术,对山楂海棠的分类地位进行了探讨。根据RAPD分子标记聚类分析,将14份苹果材料分为5类,8份山楂材料分为4类,山楂海棠的亲缘关系位于山楂和苹果之间,但与苹果较近,认为山楂海棠可能是苹果和山楂的属间杂交种。