大珠母贝组织蛋白酶D的cDNA克隆、序列特征分析及应激表达研究

通过同源克隆方法和cDNA末端快速扩增技术(RACE),获得了大珠母贝(Pinctada maxima)组织蛋白酶D基因(命名为pmCTSD)的cDNA全长序列1 742 bp,其中5'非翻译区(UTR)38 bp,3'UTR 534 bp,开放阅读框1 170 bp,编码390个氨基酸,分子量约为41.9 ku,等电...     

西葫芦抗冷性生理生化指标分析

研究了9个不同品种的西葫芦对低温的反应.结果表明,以5℃条件下幼苗叶片的电导百分率、可溶性糖的含量、脯氨酸的含量作为耐冷性鉴定指标,品种之间差异明显.可溶性糖含量与脯氨酸含量这两个指标间呈极显著相关;可溶性糖含量与电导百分率之间呈显著相关;但电导百分率与脯氨酸两个指标间的相关性不是很显著.以上3个指标可以作为西葫芦耐冷性的鉴定.     

中国南瓜主要性状配合力与遗传力分析

以12个中国南瓜高世代自交系为试验材料，采用NCⅡ设计配制36个杂交组合，分析了12个数量性状的遗传力、配合力。结果表明：在一般配合力上，6个母本的优劣顺序为Q1〉Q3〉Q4〉Q6〉Q2〉Q5，6个父本的优劣顺序为B1〉B3〉B5〉B4〉B2〉B6；亲本的一般配合力与其在杂交组合中的特殊配合力之间无必然联系；主蔓长度、可溶性固形物含量、干物质含量的狭义遗传力较高，早代选择效果较好；节间长度、单果质量、多糖含量、β-胡萝h素含量、维生素C含量、果实纵径、果实横径的狭义遗传力较低，活合存中高代讲行进择.     

迷你南瓜银叶病的发生与防治

迷你南瓜(Cucurbita moschata)银叶病典型症状表现为叶面银灰色,主要由银叶粉虱(Bemisia argentifolii Bellows&Perring)传播,发病程度因受光照、温度、湿度的影响而有所变化,据此提出迷你南瓜银叶病的有效防治措施.     

Sorption of phosphorus by some surface soils from Quebec in relation to their properties

Abstract

Surface horizons from Podzolic and Gleysolic soils were collected in various parts of the province of Quebec, Canada, and equilibrated with various amounts of KH₂PO₄ in 0.01 M CaCl₂ for 48 hours. P sorption data conformed to the linear form of the Langmuir and Freundlich equations. P solubility isotherms showed evidence of hydroxyapatite formation in most samples studied, whereas equilibration solutions of only few samples were saturated with respect to either dicalcium phoshate dihydrate or octocalcium phosphate. These reaction products were associated to soil pH and levels of added phosphate. The average values of the Langmuir sorption maximum for these studied Gleysolic and Podzolic samples were 763 and 1096 μg/g respectively. These values were higher than those obtained by the segmented and modified Freundlich models.

Relationships between the soil characteristics and P sorption parameters were evaluated by regression analysis. Among all variables, oxalate‐extractable Fe plus Al content of the Podzolic samples and the ratio of oxalate—extractable Al to clay of the Gleysolic samples gave the best significant correlation coefficients. Furthermore, soil pH and various ratios such as pyrophosphate‐extractable Fe and Al, oxalate‐extractable Fe and organic matter to clay were found to be significantly correlated only with the P sorption parameters of the Gleysolic samples.     

基于机器视觉的番茄多目标提取与匹配

果实的提取和匹配是番茄采摘机器人进行番茄定位和采摘的基础。为解决获取图像中多个成熟番茄粘连或被遮挡的情况下果实的提取和匹配问题,该文提出了使用局部极大值法和随机圆环变换检测圆算法结合进行目标提取,再使用SURF算法进行目标匹配的算法。该方法首先基于颜色对番茄进行分割提取,然后使用局部极大值法对番茄个数进行估计,结合番茄区域面积进行半径估计,之后通过随机圆环变换算法检测番茄中心和半径进行目标定位,再使用SURF算法进行双目目标匹配的算法。该方法在一定程度上解决了复杂自然环境下,多个番茄的提取和图像特征匹配的问题,并通过试验验证了其有效性和准确性,可为采摘机器人目标识别技术的研究提供参考。     

Antihyperglycemic effect of Cucurbita ficifolia fruit extract in streptozotocin-induced diabetic rats

In the present study, the antihyperglycemic effects of Cucurbita ficifolia fruit extract were investigated on streptozotocin-induced experimental diabetes in rats. Oral administration of the extract (300 and 600 mg/kg body weight, day) for 30 days resulted in a significant reduction in blood glucose, glycosylated haemoglobin, and an increase in plasma insulin and total haemoglobin. The effect was compared with 150 mg/kg b.w. tolbutamide.     

In Vitro Antioxidant Properties of <Emphasis Type="Italic">Cucurbita Pepo</Emphasis> L. Male and Female Flowers Extracts

Total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (DPPH·), hydroxyl (HO·), scavenging activities, and total phenolic values were determined in extracts of Cucurbita pepo L. female and male flowers. Powdered C. pepo L. samples were extracted in aqueous ethyl acetate (EA: W1, 17:3), ethanol (E), and water (W) by agitating in magnetic stirrer for 80°C, 15 min and also by in aqueous ethyl acetate (EA: W2, 17:3) at 25°C, 15 min. DPPH·, HO· scavenging capacities and total phenolic values of C. pepo L. female and male were higher in EA:W2 than in other extracts. In addition, all determined antioxidant capacities of female were significantly higher than male.     

不同温度对西葫芦果实冷害及生理变化的影响

通过试验确定西葫芦果实在不同温度下贮藏的安全期,并分析不同冷藏温度下其营养品质和生理生化指标的变化.结果表明,在单纯5、8、10℃低温处理下冷害出现的时间分别为处理后的7、5、6d.但5、8、10℃处理分别冷藏3、4、5d后,再在20℃环境的货架上1d就会表现出冷害;冷害对西葫芦果实可溶性蛋白含量影响不大,但会降低可溶性固形物的含量与过氧化氢酶活性,影响细胞膜透性、丙二醛和过氧化氢含量.     

Parentage analysis of huyou (Citrus changshanensis) based on internal transcribed spacer sequences

Huyou (Citrus changshanensis K. S. Chen et C. X. Fu) citrus is a natural hybrid with unidentified parents. Previous studies have hypothesized that pummelo and sweet orange might be parents, which contradicts the higher freezing resistance of huyou citrus than both the assumed parents. In this study, the parental analysis was based on internal transcribed spacer (ITS) sequences of huyou as well as six candidate parents: three mandarins, one sweet orange, one sour orange and one pummelo. Two different ITS1 sequences, with eight variable sites within the 250 bp region, were observed for huyou, which were identical only to that of sour orange and pummelo, respectively. The results indicated that huyou is most likely a hybrid between sour orange and pummelo. In addition, the application of ITS1 sequences in the phylogenetic analysis of citrus was discussed.