A method for the total collection of portal vein blood over extended periods has been developed. The method has been applied in gastrointestinal toxicology research involving rats. The method facilitated quantitative recovery of portal vein blood for 120 min for measurement and identification of absorbed radiolabeled components derived from a toxic lipphilic pesticide placed in the lumen of the small intestine. The method can be applied to any substance—nutrient, anutrient, xenobiotic chemical, or endocrine factor—absorbed into the portal vein from the gastrointestinal tract. The method (a) provides for collection of compounds absorbed from the gastrointestinal tract before distribution to nondigestive tissues (i.e., liver), (b) allows measurement of the compounds absorbed without the need to monitor flow rate, and (c) facilitates accumulation of sufficient material from the total portal circulation for analyses when submicrogram quantities are absorbed. The latter two features are important but impossible to attain with small samples of portal blood and are crucial in research with minute quantities of substrate. These features become requirements in metabolism and toxicology research. The method involves replacement of the portal blood with a suspension of perfluorohydrocarbons to substitute for the major functions of natural blood. The total-continuous portal vein fistula makes it possible to investigate metabolic and transport phenomena in live animals which previously could be explored only by in vitro methods. The method may be applied to conscious animals with further refinements. 相似文献
Abstract Plant-available P was first extracted in soils 114 years ago and a number of different analytical methods have since been developed, but for good reasons none of these methods has been adopted as a standard for all soils. With increasing cooperation within research, there is a need to harmonise the interpretation of analytical data for fertiliser recommendations, research, and environmental control. This paper evaluates the compatibility of the Swedish standard ammonium lactate (PAL) method and the widely used Olson's sodium bicarbonate (POls) method in 82 topsoil samples taken from Swedish long-term soil fertility field trials. The PAL-values were usually larger than POls, with a mean PAL/POls quotient of 2.30±1.04 (0.67–6.58). The PAL and POls means and ranges were 126±89 (5–360) and 55.1±33 (3.1–122.8) mg P kg?1 dry soil, respectively. Stepwise multiple linear regression analysis was used to evaluate the relationship between PAL and POls and how this relationship was affected by clay content, pH, and soil organic carbon content (SOC). After statistical transformation, it emerged that the square root of clay content (17.4%±13.82, range 1–54.4) and pH (6.45±0.54, range 5.5–7.7) significantly affected the relationship at partial R2-values of 2 and 12%, respectively, while ln(SOC) (2.54%±1.21, range 1–6.03) did not, apparently due the narrow range. The regressions of predicted vs. measured values explained 95 and 94% of the variation in PAL and POls, respectively. The mean deviation of predicted compared with measured values was 21.3 and 8.3 mg P kg?1 dry soil for PAL and POls, respectively, corresponding to 20 or 19% of the measured values. We conclude that a data set consisting of PAL-values can be converted into POls-values and vice versa with reasonably high accuracy when accounting for clay content and pH. 相似文献
Estimation of soil water content (SWC) in deep soil profiles is of crucial importance for strategic management of water resource for sustainable land use in arid and semi-arid zones, as well as for soil and water conservation. Soil properties have a very important effect on SWC. This study aimed to analyze the influence of soil particle size on SWC, for the first time using soil particle size to estimate SWC in deep soil profiles. SWC was measured mainly in farmland, natural grasslands and plantations of Caragana from the surface to more than 20 m depth. The same soil samples were also tested for particle size. The results show that the soil desiccation is formed in the caragana forest in 3–18 m soil layers, but almost no formation in 18–24 m layers; water content of farmland and grassland is different in all soil profiles although they are both shallow rooted plants. Correlation analysis indicated that SWC could be well predicted by clay content and the close correlation between SWC and clay content yielded a coefficient of determination ( R 2 ) of 0.82 and 0.72, respectively, for farmland and grassland. After multiple regression analysis, a regression model was built using SWC, clay content and sand content data, givingR 2 =0.66. The model provided reliable estimates of SWC profile based on textural class. This can assist in estimating water depletion by vegetation, by comparing moisture of farmland and grassland soils with that of plantation forests, and in selecting sustainable land use of arid land.View The PDF 相似文献
