切断匍匐茎对臭柏叶绿素荧光特性的影响

用荧光测定仪 (MINI-PAM)测定了切断匍匐茎对臭柏叶绿素荧光特性的影响。结果表明 :匍匐茎被切断前 (8月 15日 ) ,备切断处理的小枝与对照的PSⅡ实际光化学量子产量 (ΔF F′m)、表观光合电子传递速率 (ETR)及非光化学猝灭 (NPQ)值 ,除在 15 :0 0差异显著 (P <0 0 5 )外 ,其他时段差异不显著。切断匍匐茎处理第一天 (8月 16日 ) ,9:0 0和 18:0 0切断匍匐茎处理小枝的ΔF F′m 与对照间的差异显著 (P <0 0 5 ) ,9:0 0较对照平均低约 2 5 % ;ETR与对照间的差异虽不显著 ,但平均降幅达 13% ,午后差值减小 ;NPQ在 9:0 0和 12 :0 0与对照间的差异显著 (P<0 0 5 ) ,午前平均增幅高达 4 7% ,午后平均增幅减至 15 %。 8月 2 4日 ,切断匍匐茎处理小枝的ΔF F′m 与对照小枝之间差异显著率约为 33% (P <0 0 5 ) ,ETR的差异显著率约为 4 3% (P <0 0 5 ) ,NPQ的差异显著率约为 5 8% (P <0 0 5 )。 8月 2 4日切断匍匐茎处理样枝的气孔导度在 8:30仅为对照的 30 %左右 ,12 :0 0为 36 % ,12 :0 0后二者间的差距逐渐缩小 ,较对照平均约低 39%。 8月 19日切断匍匐茎处理样枝各部位的Fv Fm 值与对照间差异不显著 (P>0 0 5 ) ,表明不定根在消除切断匍匐茎所造成的不利干扰中发挥了明显的作用。     

毛乌素沙地臭柏年轮生长动态的研究

以毛乌素沙地天然臭柏为材料 ,用实体解剖镜和光学显微镜研究观察臭柏的年轮形态特征 ,应用树干解析法研究毛乌素沙地沙丘顶部臭柏和滩地臭柏的径向生长和伸长生长动态。结果表明 :沙丘顶部臭柏偏心生长明显 ,长径与短径比为 14 0 .36 % ,伪年轮发生频率为 12 .0 7% ;滩地臭柏偏心生长不明显 ,长径与短径比为 10 5 .2 2 % ,伪年轮发生频率为 1.80 % ;根据年轮数推断 ,沙丘顶部臭柏群落的年龄至少有 12 3a以上 ,滩地臭柏年龄有 5 0a以上 ;沙丘顶部臭柏径生长缓慢 ,生长过程中没有明显的峰值 ,年平均生长量为 0 .94mm ,累积生长曲线的S型特征不明显 ;滩地臭柏的径生长高峰出现在 35年 ,年平均生长量为 1.5 1mm ,是沙丘顶部臭柏的 1.6倍 ,累积生长曲线的S型特征也不明显 ;沙丘顶部臭柏的匍匐茎长度与年龄之间表现出指数函数关系 ,而滩地臭柏的匍匐茎长度与年龄之间存在着显著的线性相关关系     

黄金间碧玉竹引种试验

通过对黄金间碧玉竹的繁殖和适应性试验，认为黄金间碧玉竹繁殖容易，适应性强．在亚热带轻霜地区种植，生长良好，可作观赏和材用。影响其生长发育的主要限制因子是霜冻，若定檀当年和次年采取防护措施，待其根系、地下茎和茎杆抗性增强后，便可抵御一定程度的寒窖。     

PEG与低温对法国百里香种子萌发的影响

以法国百里香(Thymus vulgaris)为材料,研究了PEG模拟干旱胁迫和低温对种子萌发和幼苗生长的影响,旨在探索进口百里香种子播种育苗的适宜条件。结果表明,温度、干旱及其互作对法国百里香种子萌发和幼苗生长均有显著影响。0~10%PEG-6000水分条件下,8~24℃下法国百里香种子均能良好萌发,其中在10~12℃时发芽率最高,达79.3%,显著高于其余各处理(P0.05)。当PEG-6000达15%时显著抑制其萌发,至30%PEG-6000时仅在22~24℃下少量萌发。萌发后,当温度为22~24℃,根长、茎长均显著高于其他处理。种子和幼苗生长均表现出对PEG模拟干旱胁迫和低温不同的响应。     

遮荫处理对铁尾矿基质臭柏实生苗快速叶绿素荧光特性的影响

【目的】通过比较分析铁尾矿基质下不同遮荫处理间臭柏实生苗叶片原初光化学反应的差异,揭示臭柏在不同光照条件下的光保护机制,从理论上解释其天然更新过程,为臭柏光胁迫的研究提供理论依据,也为臭柏在铁尾矿废弃地造林提供技术依据。【方法】以唐山迁安铁尾矿为基质,通过盆栽遮荫试验,利用快速叶绿荧光诱导动力学测定与 JIP-test数据分析方法,分别测定0,25%,50%,75%和90%5个遮荫处理下臭柏叶绿素8项荧光参数。【结果】1)全光照条件下Fo 增大,Fm 减小,而叶片光合系统量子产额和能量分配比率(φPo,Ψo和φEo)、单位面积光合机构的比活性参数(Fm,ETo/CSm和 TRo/CSm)和单位面积内反应中心的数量(RC/CSm)均最小,且各个遮荫处理组间差异均达到显著(P＜0.05)或极显著水平(P＜0.01),表明全光照条件下臭柏实生苗反应中心失活,受到光抑制,而全光照下快速叶绿素荧光诱导动力学曲线中 K 点与 J 点明显高于其他遮荫处理,而且光抑制对臭柏叶片PSⅡ的供体侧(OEC)有伤害,造成PSⅡ受体侧(Q －A )的大量积累,阻碍电子传递链向Q －A 下游传递。2)全光照条件下叶片热耗散的量子比率(φDo )和单位面积光合机构的比活性参数( DIo/CSm )均最大,且与其他遮荫处理组间差异也均达到显著(P＜0.05)或极显著水平(P＜0.01),表明全光照条件下臭柏在遭受光抑制后,通过增加天线色素的热耗散,增加非辐射能量耗散等一系列机制来保护光合机构免受过量光能的危害。3)随着遮荫率增加,φPo ,Ψo和φEo,Fm(ABS/CSm),ETo/CSm和TRo/CSm,RC/CSm随之上升,而φDo和Fo(ABS/CSo),DIo/CSm随之下降,90%遮荫处理表现出明显的光捕捉特征,50%遮荫处理 Fm ,PIABS的平均值最大,说明该遮荫条件下臭柏叶片光抑制最小,光合能力最强,其他参数在不同遮荫处理组间无明显差异( P＜0.05)。【结论】臭柏幼苗若无遮荫措施,受到光胁迫,而其通过增加非辐射能量耗散途径达到光抑制防御的机制。因此臭柏天然更新从乌柳灌丛下开始,逐渐脱离乌柳灌丛的保护,完全暴露在全光照条件下,进而实现自身的不断生长、壮大,但在废弃地上植苗造林初期尚需采取一定的遮荫措施,以提高造林成活率。     

Colouring ornamental fish (Cyprinus carpio and Carassius auratus) with microalgal biomass

Koi carp and goldfish value increases with intensity of skin colour, which is an important quality criterion. Fish cannot fully synthesize their own carotenoid colourings and these must therefore be included in their diet. Two trials were undertaken to investigate skin colour enhancement in ornamental species (i.e. three chromatic varieties of koi carp (Cyprinus carpio), namely Kawari (red), Showa (black and red) and Bekko (black and white) and goldfish (Carassius auratus)) by feeding a dietary carotenoid supplement of freshwater microalgal biomass [Chlorella vulgaris, Haematococcus pluvialis, and also the cyanobacterium Arthrospira maxima (Spirulina)], using a diet containing synthetic astaxanthin and a control diet with no colouring added for comparison. In the first trial, five homogeneous duplicate groups of 25 juvenile koi carp (C. carpio) (initial mean body weight 24.6 ± 0.7 g) were fed, for 10 weeks, one of the four diets containing 80 mg colouring/kg diet. In the second trial, this procedure was repeated for five homogeneous duplicate groups of 25 goldfish (C. auratus) (initial mean body weight of 0.9 ± 0.1 g). Initial and final samples of skin along the dorsal fin were withdrawn, from five fish per group, for subsequent analysis of total carotenoid content (spectrophotometric analysis), and red hue (colorimetric analysis, CIE (1976) L* a* b* colour system). Growth and feed efficiency were not significantly different between groups administered by the various dietary treatments. In both trials, dietary carotenoid supplementation increased total skin carotenoid content. The more efficient colouring for koi carps was found to be C. vulgaris biomass, providing both maximum total carotenoid deposition and red hue for the three chromatic koi carp varieties studied, and particularly for the kawari variety. For goldfish the best colouring obtained, as ascertained by total carotenoid content, was also achieved using C. vulgaris biomass, and red hue was maximum when using H. pluvialis biomass.     

Validation of the otolith increment deposition ratio using alizarin marks in juveniles of the sparid fishes, Diplodus vulgaris and D. puntazzo

Deposition rate of otolith increments was validated by two immersions in alizarin complexone 10 days apart in juvenile Diplodus vulgaris and D. puntazzo. Alizarin complexone produced a well-defined scarlet band on the otoliths of all individuals. One increment:one day ratio was found for both species.     

Pigmentation of gilthead seabream, Sparus aurata (L. 1875), using Chlorella vulgaris (Chlorophyta, Volvocales) microalga

A feeding experiment was conducted over 9 weeks with seven groups of 30 (fish per group) unpigmented gilthead seabream, Sparus aurata (L. 1875) (initial mean weight = 145.2 ± 12.3 g). Three experimental diets were prepared by adding to a basal diet free of carotenoid (final pigment content of around 40 mg per kg feed): (i) a biomass of the carotenogenic Chlorella vulgaris (Chlorophyta, Volvocales); (ii) a synthetic astaxanthin; and (iii) a mixture (1:1) of microalgal biomass and synthetic astaxanthin. At 3‐week intervals, five fish were sampled from each tank for total carotenoids analysis in skin and muscle. The carotenoid pigments (total amount = 0.4%) identified in the carotenogenic alga were lutein (0.3%), β‐carotene (1.2%), canthaxanthin (36.2%), astaxanthin, free and esterified forms (55.0%), and other pigments (7.3%). Carotenoid pigments were significantly deposited in the four skin zones studied during the feeding trial: the forefront between the eyes, the opercule, along the dorsal fin and in the abdominal area. In the muscle, regardless of the astaxanthin source, the amount of carotenoids measured was very low (less than 1 mg kg^?1) and differences not significant. Moreover, no muscle pigmentation was evident, and there was no variation in the amount of carotenoid analysed in skin tissue, through the trial, for each treatment. It was concluded that supplementing the feed with C. vulgaris would be an acceptable practice in aquaculture to improve the market appeal of the gilthead seabream.     

Nodule phosphorus requirement and O2 uptake in common bean genotypes under phosphorus deficiency

Abstract

The effect of P deficiency on nodulation, nodule P content, nodule O₂ permeability and N fixation rates in Phaseolus vulgaris–rhizobia symbiosis was studied under glasshouse conditions. Four recombinant inbred lines (L34, L83, L115 and L147) and one variety cultivated in Morocco (Concesa) were inoculated with Rhizobium tropici CIAT 899 in hydroaeroponic culture. Two P levels i.e. 75 (deficient level) and 250 µmol plant^?1 week^?1 P (sufficient level) were applied and the trial was assessed 42 days after transplanting that coincide with plant flowering stage. Under P-deficiency, decrease of plant growth (18%) and nodule biomass (19%) was detected and significantly pronounced in the sensitive line L147 compared with the remaining genotypes. Additionally, under P-deficiency, the efficiency in use of rhizobial symbiosis, estimated by the slope of the regression model of shoot biomass as a function of nodule biomass, was significantly increased in the four lines. This constraint did not significantly influence nodule P content in Concesa, but it was 24 and 41% lower in the tolerant and in the sensitive lines, respectively. Nodule P content was positively correlated to nodule biomass, r=0.75, and shoot N, r=0.92. These genotypic variations were associated with variability in nodule O₂ permeability that was significantly affected by the P level-bean genotype interaction. Under P-deficiency, nodule O₂ permeability was significantly reduced in the tested genotypes and accompanied with a decrease in shoot N content, especially in the sensitive lines (35%). Moreover, the ratios plant N fixed: nodule P content and plant N fixed:nodule dry weight were affected under P-deficiency in four lines with an exception observed in Concesa. Depending on the observed data we concluded that N₂ fixation efficiency could be influenced by nodulation and level of nodule P requirement which depend on both bean genotypes and P level.     

驱除牛肝片形吸虫及消化道圆形线虫与体增重相关性的研究

通过寄生虫区系调查和临床检验，发现肝片形吸虫及消化道圆形线虫感染普遍。因此，应用硝氯酚和左旋咪唑给牛驱虫，并观察牛的虫卵减少率与体增重的关系。结果用药组的体增重与对照组比，硝氯酚组平均多增重２６．１ｋｇ／头；左旋咪唑７８．９ｋｇ／头。