盐胁迫下构树DREB转录因子基因表达的实时荧光定量PCR分析

转录因子是指那些专一性地结合于DNA特定序列上、能激活或抑制其他基因转录的蛋白质(王少峡等,2004)。DREB(dehydration responsive element binding protein)转录因子是一种特异性的转录因子,当植物在干旱、低温及盐等逆境胁迫下,DREB转录     

Computed Tomographic Features of Choanal Atresia in a Friesian Foal

A 3-week-old Friesian colt with a history of respiratory distress since birth was presented to our facility. Results after an endoscopy showed obstruction of the right nasal passage to the nasopharynx. Computed tomography was performed to further characterize this unilateral blockage. The images demonstrated a complete membranous obstruction of the ventral meatus, along with deviation of the vomer bone toward the left side of the nasal cavity. The diagnosis of unilateral choanal atresia was confirmed. This report is the first to describe computed tomographic features of choanal atresia in a foal.     

EFFECTS OF ANESTHETIC PROTOCOL ON NORMAL CANINE BRAIN UPTAKE OF 18F‐FDG ASSESSED BY PET/CT

The purpose of this study was to assess the effects of four anesthetic protocols on normal canine brain uptake of 2‐deoxy‐2‐[¹⁸F]fluoro‐d ‐glucose (FDG) using positron emission tomography/computed tomography (PET/CT). Five clinically normal beagle dogs were anesthetized with (1) propofol/isoflurane, (2) medetomidine/pentobarbital, (3) xylazine/ketamine, and (4) medetomidine/tiletamine–zolazepam in a randomized cross‐over design. The standard uptake value (SUV) of FDG was obtained in the frontal, parietal, temporal and occipital lobes, cerebellum, brainstem and whole brain, and compared within and between anesthetic protocols using the Friedman test with significance set at P<0.05. Significant differences in SUVs were observed in various part of the brain associated with each anesthetic protocol. The SUV for the frontal and occipital lobes was significantly higher than in the brainstem in all dogs. Dogs receiving medetomidine/tiletamine–zolazepam also had significantly higher whole brain SUVs than the propofol/isoflurane group. We concluded that each anesthetic protocol exerted a different regional brain glucose uptake pattern. As a result, when comparing brain glucose uptake using PET/CT, one should consider the effects of anesthetic protocols on different regions of the glucose uptake in the dog's brain.     

PET/CT today and tomorrow in veterinary cancer diagnosis and monitoring: fundamentals,early results and future perspectives

Functional imaging using positron emission tomography (PET) plays an important role in the diagnosis, staging, image‐guided treatment planning and monitoring of malignant diseases. PET imaging complements conventional anatomical imaging such as computed tomography (CT) and magnetic resonance imaging (MRI). The strength of CT scanning lies in its high spatial resolution, allowing for anatomical characterization of disease. PET imaging, however, moves beyond anatomy and characterizes tissue based on functions such as metabolic rate. Combined PET/CT scanners were introduced commercially in 2001 and a number of technological advancements have since occurred. Radiolabelled tracers such as ¹⁸F‐fluorodeoxyglucose (FDG) and ¹⁸F‐fluorothymidine (FLT) allow visualization of various metabolic processes within cancer cells. Many studies in human oncology evaluating the utility of PET/CT have demonstrated clinical benefits. Few veterinary studies have been performed, but initial studies show promise for improved detection of malignancy, more thorough staging of canine cancer and determination of early response and disease recrudescence.     

临床医学系统术语SNOMED CT的特点及其应用

SNOMED CT已经成为国际上广泛关注的一种医学参考术语与信息编码。介绍了SNOMED CT的发展历史，着重介绍与分析了其核心内容与特点，最后介绍了SNOMED CT的应用情况。     

模拟冻融循环对黑土黏化层孔隙结构的影响

以东北典型黑土区耕地土壤为研究对象,采用室内模拟冻融以及CT(Computerized tomography)扫描相结合的方法,通过孔隙数目、平均面积、成圆率3个指标的量化与分析,研究了冻融循环对黏化层土壤孔隙特征的影响规律,以期为探明该区土壤侵蚀机理提供科学依据。研究结果表明,冻融循环不同程度地降低了原状土和填充土的孔隙数目和面积,均在循环3次时达到最低值,冻融循环对≥5 mm孔隙影响较为明显。相对原状土而言,前期含水量对黏化层填充土柱孔隙特征的影响较小。黏化层结构对模拟冻融循环的响应特征印证了季节性冻融循环对黑土区黏化层可蚀性的影响,进而诱发土壤侵蚀、促进沟蚀发展的可能。      

利用计算机断层扫描技术研究土壤改良措施下土壤孔隙

为探明不同土壤结构改良措施（秸秆覆盖、免耕、有机肥、保水剂）对土壤孔隙特征及分布的影响,采用计算机断层（computed tomography,CT）扫描法定量分析了土壤孔隙的数目、孔隙度及孔隙在土壤剖面上的分布特征。结果表明：不同措施均提高了土壤总孔隙数、大孔隙数及0.13～1.0 mm孔隙数,且其孔隙度也相应提高。同时孔隙成圆率也得到了改善。各处理中以有机肥和免耕处理效果较佳,其次为保水剂和秸秆覆盖,对照最低。此外,不同措施显著提高了土壤的田间持水量和＞0.25 mm 水稳性团聚体含量,降低了土壤容重,且各处理中,仍以有机肥和免耕处理效果最佳,其田间持水量分别较对照提高了15.9%和16.4%,而土壤容重较对照降低了6.8%和8.8%。相关分析表明：田间持水量、容重和＞0.25 mm水稳性团聚体含量与土壤总孔隙度和大孔隙度呈显著或极显著正相关;而土壤容重对于总孔隙度和大孔隙度及孔隙成圆率呈显著负相关。