用RAPD技术筛选中国荷斯坦牛产奶量性状遗传标记

选用320条10碱基随机引物在由36头高产（305d产奶量〉8500kg）和32头低产（305d产奶量〈5600kg）中国荷斯坦牛所组成的2个DNA池间进行RAPD-PCR扩增，从中筛选出稳定性好、带型清晰且在2个DNA池间有明显差异的RAPD引物24条。用筛选到的24条引物对所有个体进行PCR检测，得到了5个与奶牛产奶量性状相关的RAPD标记，并对其中4个进行了克隆测序和SCAR标记转化以及生物信息学分析。结果表明：SCAR标记yield—s139与产奶量密切相关，应用电子克隆方法已将该标记由921bp延伸到2141bp，经同源性比较发现，此标记对应于奶牛基因组中LINEs家族中的一个重复元件，推测该元件附近可能存在与产奶量性状相关的QTLs或主效基因。     

不同条件下蒙古冰草种子产量及产量构成因子的分析

在西苏旗对蒙古冰草的种子产量及产量构成因子进行了分析得出结果为冰草的实际种子产量与潜在种子产量差别很大，蒙古冰草种子产量的主要限制因子为抽穗率和结实率。     

Evaluation of Five Most Commonly Grown Cotton Cultivars (Gossypium hirsutum L.) Under Mediterranean Conditions: Productivity and Fibre Quality

Studies were conducted in four regions of central Greece (Larisa, Karditsa, Livadia, Thiva), to evaluate the productivity and fibre properties (fibre strength and fibre length) of the five most commonly grown cotton cultivars in these regions. The experiments lasted for 6 years (1995–2000). All the characteristics tested were strongly influenced by the factors ‘year’, ‘cultivar’ and ‘region’. Significant cultivar × region, year × cultivar, region × year and cultivar × region × year interactions occurred for all the characteristics studied. The cultivar Alegria had the highest seed‐cotton and lint yield, while Aria had the lowest ones. Both cultivars gave the highest mean lint percentage but the lowest mean boll weight (MBW). Alegria had the lowest fibre strength, while Aria had the highest. The Acala‐type cultivars, Acala SJ2 and Zeta 2, had intermediate seed‐cotton and lint yield, while Vered 171 had high seed‐cotton and satisfactory lint yield. The three cultivars mentioned above had the highest MBW and very good fibre strength. Positive correlation was observed between fibre strength and length. In spite of the fact that mean fibre length was statistically the same in all cultivars, it was influenced by the factor ‘year’, as a result of different weather conditions. The year 1996 was the most unfavourable one for seed‐cotton yield and MBW, because it was characterized by lower than average air temperatures and rather dry conditions for all study sites. The factor ‘region’, a combination of different climatic conditions and cultural practices, influenced significantly all the parameters studied.     

夏直播花生规范化栽培密度与氮肥优化配置研究

采用二次饱和D—最优设计建立了密度和N肥用量与夏直播花生产量的数学模型。进一步分析表明，密度与N肥用量呈负交互效应，肥料用量增加，花生密度可适当减少。密度与N肥用量的最优组合为每公顷播15．36万穴，施纯N99．6kg，最高产量可达到5527．0kg。公顷产量在4500~5527kg范围内的措施组合为：每公顷播15．O～18．7万穴，施纯N65．1～124．4kg。     

杉木林杆材阶段能量积累和分配的研究

目前森林生态系统生产力的研究 ,多以干物质量作为指标 ,这对深入了解生态系统的功能 ,生态效益都有一定的局限性。必须把生物量与能量结合起来 ,因为生物量和能流的研究 ,是人工林生态系统结构和功能的研究中最主要的一环 ,是研究物质生产、物质循环和能量流动的基础。关于森林各组织器官的热值和能量的研究 ,国内外许多学者做过这方面的工作。刘世荣等 (1990 )研究了落叶松 (Larixgmelinii)林群落能量积累、分配、固定和转化的规律 ;陶金川等 (1990 )探讨了银鹊树 (Tapisciasinensis)群落的生物量和能量的现存量 ;张文其等 (1995 )对鹤山…     

Physiological Analysis of Yield Variation in Mungbean (Vigna radiata [Roxb.] Wilczek)

Two mungbean varieties, Guj-1 and PIMS-1 differing in their productivity potential, were examined to investigate their photosynthetic system at various stages of growth in relation to yield with two different dates of sowing. Vertical leaves were found to be beneficial. On the other hand, excessive leaf area during the later stages of growth may be detrimental to yield. Lower LAD at seedling stage and overall high NAR values may be reflected in higher grain yield. NAR declined as season progressed and this, in turn, may be related with increase in LAI. The grain yield was considerably more at the first date of sowing as compared to second date of sowing, the effect was being more pronounced in var. PIMS-1 . Various environmental factors recorded at two different dates of sowing and at various stages of growth indicated that these factors may have influenced the yield potential at two different dates of sowing.     

灌水量对不同类型晚稻生育及产量影响的初步研究

采取盆栽遮雨人工定量灌水的方法,探讨了常规晚稻和杂交晚稻前后两大生育时期不同灌水量对生育和产量的影响.结果表明:两类型晚稻品种前后两个生育时期节水灌溉都能达到高产之目的,但不同类型和不同生育时期的最佳灌水量和节水效果不同.     

Influence of the Indoleacetic Acid-Lysine Synthetase Gene (iaaL) of Pseudomonas syringae subsp. savastanoi on Yield Attributes of Potatoes

The iaaL gene of Pseudomonas syringae subsp. savastanoi encodes an indoleacetic acid-lysine synthetase which conjugates free indoleacetic acid (IAA) with lysine. lAA-lys is biologically less active than free IAA. The iaaL coding region was expressed under the control of the cauliflower mosaic virus 35S promoter and transgenic potato plants were produced (Spena et al. 1991). 35S iaaL potato plants are characterized by increased internodal length and epinastic bending of older leaves. In three greenhouse experiments with plants grown in pots of different size and in two growth chamber experiments tuber number increased in iaaL transgenic plants compared to untransformed and vector-transformed controls of the same genotype. The increase in tuber numbers observed under controlled conditions was reflected in tuber yield which increased in the pot grown transgenics.     

多花黑麦草播期试验(1987～1988年)

本试验研究了7个不同播期对多花黑麦草出苗、生产性能的影响.结果表明:在江西清江种草养畜试验站从8月中旬至11月中旬均可播种多花黑麦草,尤以9月中旬至10月中旬更为适宜.     

Effect of Nitrogen Rate and Stubble Height on Dry Matter Yield,Crude Protein Content and Crude Protein Yield of a Sorghum–Sudangrass Hybrid[Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf.] in the Three‐Cutting System

In this study, the effects of nitrogen (N) rate (60, 120, 180 and 240 kg N ha^?1 applied in three equal dressings at seeding and after the first and second cuttings) and stubble height (7, 14 and 21 cm) on the dry matter (DM) yield, crude protein (CP) content, and CP yield of a sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf., cv. Pioneer 988] in the three‐cut system was investigated. The N rate had no significant effect in the first and third cuttings, but in the second cutting DM yields increased significantly with increase in N rate. The highest yield of 9.1 t ha^?1 was obtained with 80 kg N ha^?1 for the average of 2 years at the second cutting, but no significant difference was found among the 40, 60 and 80 kg N ha^?1 rates. CP content and yield were not significantly affected by N rate at the first and third cuttings, but CP content and yield were significantly affected by application of N at the second cutting. Stubble height had a significant effect on CP content at the third cutting. However, it had no significant effect on CP content at the first and second cuttings. Stubble height had a significant effect on the CP yield at the first cutting, but no significant effect on CP yield at the second and third cuttings.