Purification and Characterization of a New DPPH Radical Scavenging Peptide from Shrimp Processing By-products Hydrolysate

A peptide with high antioxidant activity was isolated and identified from shrimp processing by-products hydrolysate. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was used to evaluate the antioxidant activity of fractions. The purified antioxidant peptide was identified as Ser-Val-Ala-Met-Leu-Phe-His (804.4 Da) by electrospray ionization tandem mass spectrometry. The purified peptide at 50 μg/mL showed antioxidative activity of 65.7 ± 0.9%, which was 3.18-fold higher compared with the first step separation by ion-exchange chromatography. It is possible to produce natural antioxidative peptides from shrimp processing by-products hydrolysate. The high antioxidant activity may be due to the presence of Phe-His segment at the C-terminus of the peptide.     

Nutrient Compositions and Properties of Antarctic Krill (Euphausia superba) Muscle and Processing By-Products

ABSTRACT

This study investigated the morphometrics, proximate chemical compositions, pH, total amino acid (TAA), fatty acid profile, and minerals of the processing by-products of Antarctic krill (Euphausia superba). The nutrient compositions and properties of the by-products were revealed by being compared to those of Antarctic krill muscle and the economically important species of freshwater prawns (Macrobrachium nipponense) and penaeid shrimps (Metapenaeus ensis). The by-products are worthy of utilization because of the high ratio to the total weight (65.7%). The crude protein contents in the muscle and by-products of krill are 17.4 and 11.7%, respectively. The krill proteins have higher contents of essential amino acids (EAAs). The EAAs constitute 42 and 37% of the TAAs in muscle and by-products, respectively. The krill processing by-products contain high levels of total lipid (3.3%), and polyunsaturated fatty acids constitute 34% of fatty acids with high levels of eicosapentaenoic acid (EPA; 19.08%) and docosahexaenoic acid (DHA; 10.02%). Krill meat provides considerable iron, zinc, calcium, selenium, and copper. It is imperative to lower the fluoride level (70.1 mg/kg, wet basis) in krill muscle. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) reveals that myosin heavy chain (MHC) and actin are the major proteins in muscle, and their contents vary between species.     

Chemical composition and in sacco dry matter degradability of residue and by-products of palm fruit processing in the rumen of steers

The nutritive value of residue (palm calyx leaves (PCL)) and by-products (palm press fiber (PPF) and palm oil sludge (POS)) of palm fruit processing were studied through analysis of their chemical components and degradability of their dry matter (DM) in the rumen of steers. Chemical analysis showed that the materials have similar organic matter components (mean = 95.2%), while crude protein (CP, g/100 g) and metabolisable energy (ME, MJ/kg DM) were highest in POS (10.02 and 9.43), followed by PPF (7.02 and 8.61) and least in PCL (5.42 and 8.04). An opposite trend of CP and ME was noticed in NDF and ADF contents, being 61.53 and 49.11% in PCL, 44.84 and 32.08% in PPF and 25.35 and 20.29% in POS. Mineral contents showed that PPF and POS had the lowest and highest concentration, respectively, of Mg (0.07 vs. 0.11%), Cu (58.5 vs. 143.9 mg/kg) and Fe (1374.5 vs. 4086.0 mg/kg). Dry matter degradation characteristics and effective degradability varied significantly ( P  < 0.05) and were consistently highest in POS and least in PCL. Results have generally shown that the residue and by-products could be ranked for their potential feeding value as POS > PPF > PCL. It is concluded that POS and PPF can be harnessed directly as feed resources for ruminant animals, while PCL will require some treatments to enhance its nutritive value in ruminants.     

Apparent digestibility coefficients of nutrients in brewer's and rendered animal by-products for rainbow trout (Oncorhynchus mykiss (Walbaum))

Two experiments were conducted to measure the apparent digestibility coefficients (ADCs) of nutrients, dry matter, and energy in by‐products of the brewing industry and in selected animal protein ingredients for rainbow trout. In experiment 1, 500 rainbow trout (average body weight 170.8±5.5 g) were stocked in ten 140‐L digestibility tanks with 50 fish per tank and two tanks per diet. Yttrium oxide was used as an inert marker in the diets. The high fibre content of brewer's dried grains (BDG) affected the ADCs of dry matter but not of protein or amino acids. Brewer's dried yeast had a higher protein content than BDG, but ADC values for protein and amino acids were significantly lower. The ADCs of phosphorus were similar among brewer's dried products. The BDG high‐protein, fraction had marginally higher ADC values for dry matter, protein and amino acids than regular BDG. Conditions in experiment 2 were similar to those in experiment 1. ADC values for spray‐dried porcine plasma were over 98% for dry matter, crude protein, crude fat, and gross energy. ADC values for spent hen meal were higher than those of poultry by‐product meal or feather meal, with the exception of gross energy. However, spent hen meal was unpalatable. The ADCs in these ingredients were variable, and this variability must be taken into account when these ingredients are formulated into feeds for fish.     

Influence of the proportion of poultry slaughter by-products and of untreated or hydrothermically treated legume seeds in diets for rainbow trout, Oncorhynchus mykiss (Walbaum), on apparent digestibilities of their energy and organic compounds

Apparent digestibilities (D) of energy and proximate nutrients were determined in rainbow trout, Oncorhynchus mykiss (Walbaum), by stripping faeces and using Cr₂O₃ as a marker. When wheat gluten + L-lysine-HCl (WG-lys) as sole source of dietary N were increased stepwise from 324 to 941 g·kg^?1, replacing 210 g·Kg^?1 fish oil (FO) and 407 g·kg^?1 gelatinized corn starch (GCS), D of crude protein rose from 96 to 99% and that of FO was always above 96%. D of GCS was 70% at inclusions up to 210 g·kg^?1, but only 41 at 407 g·kg^?1. Three by-products of poultry slaughtering and three legume seeds replaced WG at levels of 250 and 500 g·kg^?1 in a basal diet of WG-lys, minerals and vitamins. Soy beans had been pressure cooked at one of two intensities, and field beans and fields peas were either untreated or had been autoclaved. D of energy and crude protein was hardly affected by dietary proportion in poultry blood mean (84 and 85%), poultry offal meal (80 and 81%) and incompletely pressure cooked soy beans (82 and 90%); increasing dietary proportions from 250 to 500 g·kg^?1 reduced the respective values of feather meal (88–81 and 86–83%), raw (43–32 and 76–70%) and autoclaved field beans (67–57 and 89–86%), as well as raw (47–39 and 86–83%) and autoclaved field peas (65–40 and 91–86%). At the higher proportion, D of energy of completely pressure cooked soy beans was reduced from 91 to 86%, but that of crude protein was constant at 94%.     

Evaluation of fisheries by-catch and by-product meals in diets for red drum Sciaenops ocellatus L.

This study evaluated various by‐catch and by‐product meals of marine origin with red drum (Sciaenops ocellatus L.). Four different kinds of by‐catch or by‐product meals [shrimp by‐catch meal from shrimp trawling, Pacific white shrimp (Litopenaeus vannamei (Boone)) processing waste meal, red salmon (Oncorhynchus nerka (Walbaum)) head meal, and Pacific whiting (Merluccius productus (Ayres)) meal] were substituted for Special Select? menhaden fish meal at 33% or 67% of crude protein in diets formulated to contain 40% crude protein, 12% lipid, and 14.6 kJ digestible energy g^?1. Each of these diets and three additional diets consisting of shrimp processing waste meal formulated on a digestible‐protein basis and two Pacific whiting diets containing reduced levels of ash were also evaluated in two 6‐week feeding trials with juvenile red drum (initial weight of 4–5 and 1–2 g fish^?1 in trials 1 and 2). Red drum fed by‐catch meal at either level of substitution performed as well as fish fed the control diet; whereas, fish fed shrimp processing waste meal diets had significantly (P≤0.05) reduced weight gain and feed efficiency ratio values compared with the controls, even when fed on a digestible‐protein basis. The diets containing Pacific whiting at either levels of substitution and regardless of ash level supported similar performance of red drum as those fed the control diet. Fish fed the red salmon head meal diet fared poorly, probably owing to an excessive amount of lipid in the diet that became rancid. Overall, by‐catch meal associated with shrimp trawling and Pacific whiting appear to be suitable protein feedstuffs for red drum.     

新疆籽瓜副产物加工利用研究现状及前景

籽瓜是西瓜的分支品种,丰富的籽瓜资源是制作炒瓜子的主要原料。取籽后的籽瓜瓜皮和瓜瓤中含有大量的氨基酸、维生素和矿物质等营养成分,充分利用籽瓜废弃物有利于提高籽瓜产品的附加值。本文介绍籽瓜的营养成分,综述籽瓜副产物加工利用的研究现状,展望籽瓜及其加工产业的发展前景。     

低值水产品及水产副产品的加工与综合利用

低值水产品及其副产品通常经过简单加工后被作为饲料利用或直接作为废物丢弃,不仅造成资源浪费,而且造成对环境的污染。研究表明,利用高新技术,可以把低值水产品或水产品下脚料加工成功能性鱼油制品、新型鱼糜制品、海鲜调味品和营养丰富的水解鱼蛋白饲料等高附加值的产品,以及骨糊、硫酸软骨素和骨胶等功能性添加剂。这些产品的研究开发为低值水产品及副产品的加工利用提供了新的途径。     

Short-term responses of soil decomposer and plant communities to stump harvesting in boreal forests

Recently, in addition to logging residues, stumps have become an important component in energy production since there is growing global interest in the use of renewable energy sources in order to decrease anthropogenic carbon emissions. Harvesting of stumps influences the forest floor by changing vegetation and soil organic layers and exposing mineral soil across large areas. We studied whether stump harvesting after clear felling poses further short-term changes in boreal forest soil decomposer community (microbes and mesofauna) and vegetation when compared to the traditional site preparation practice (mounding). In general, stump harvesting caused decline in enchytraeid abundance but did not induce further major changes in decomposer community otherwise nor in vegetation of each soil micro-habitat (intact soil and exposed mineral soil). However, the abundances of almost all decomposer animals were lower in the exposed mineral soil than in the intact soil. Stump removal increased the area of exposed mineral soil in the clear felled areas, leading to lower amount of high quality habitat for most decomposer organisms. Hence, it is obvious that there are (or will be) differences in the decomposer community dynamics between the treatments at the forest stand level. Both species richness and coverage of plants benefitted from large-scale exposure of mineral soil. Because the stump removal procedure disturbs soil organic layers and negatively affects the decomposer community, it has the potential to alter nutrient dynamics in forests.     

无土栽培基质原料的改性方法综述

简述了专利申请文件中涉及的可用作无土栽培基质原料的农林工矿业副产品、废弃物种类,以及其作为无土栽培基质原料利用时进行改性的方法,并对该类物质资源化利用前景进行了展望。