Engineering Calculation Mode Based on Reformative B/S Architecture

Engineering designed mode has entered a new stage because of the development of network. New calculating system based on network appeared. By analyzing the common B/S architecture, this paper brings forward an reformative architecture based on Web services and ASP. NET in order to build the new mode of engineering calculation. It can fully implement the sharing of the resources and provide the engineering designers a wonderful calculating environment, and the working eifficiency of designers can be enhanced.     

基于B/S结构的数字地质图元数据信息系统设计与实现

以国土资源环境与区域经济信息系统信息共享元数据内容标准草案(“NREDIS”)为依据,结合ASP.NET技术,利用C#语言,设计了一个基于B/S(浏览器/服务器)结构的数字地质图元数据信息系统。实现了元数据标准的浏览和元数据的输入、查询及维护4大功能。研究成果为国家空间信息基础设施和数字地球的建设提供了一定的理论基础和技术原型,推动了地理空间信息的网络发布和共享。     

基于BIM技术与B/S架构的大坝安全可视化监测系统

为了实现大坝整体结构、安全监测测点布置及监测成果的可视化展示功能,并且能够及时、准确地掌握大坝运行状态和异常情况,以甘再碾压混凝土重力坝为例,对传统大坝安全监测系统存在的问题进行了深入的分析与解答,并围绕问题提出了可视化的大坝安全监测系统思想,利用Revit、Dynamo、Civil3D等主流BIM软件建立了大坝主体结构及监测点的模型族库,集成了大坝及监测系统的三维可视化BIM模型.借用于BIMFACE平台轻量化技术、PHP脚本语言和MySQL数据库等现代化信息技术,开发出基于B/S架构的甘再大坝安全可视化系统,实现了BIM模型的Web轻量化展示,可通过客户端浏览器在三维模型中直观查看大坝结构、监测仪器信息以及监测点时程曲线变化等内容.通过本系统的应用,大大提高了大坝运行期间安全监测的可视化管理水平,同时对传统的大坝安全监测系统提出了现代化的建立方式与思想.     

干旱区B/S模式水库调度系统设计

利用Visual Studio．NET的优良集成开发环境、采用SQL Server 2000作为B／S计算环境数据库服务器的分布式数据管理系统。针对干旱区水库调度的实际特点，选择了适合当地实际特点的预报、调度模型，考虑到该系统维护、升级的需要，系统采用组件技术，对各子系统进行功能分区，模块化处理。系统具备基本信息接收、存储和查询，洪水预报，水位预报，水库调度，成果管理和数据库管理等主要功能。系统界面简洁、友好，功能适用，方便操作。结合实例展示了系统的在干旱区的应用情况以及在进一步推广应用前景。     

Visits to the U.S. Bureau of Reclamation from South Asia and the Middle East, 1946–1990: An indicator of changing international programs and politics

International study programs have contributed to the diffusion of modern irrigation technologies, approaches, and problems. But the patterns and processes of international travel have received little attention to date. This paper examines foreign visits to the US Bureau of Reclamation (USBR) from South Asia and the Middle East from 1946 to 1990. Using data from the Foreign Activities Branch of the USBR, we compare the participation rates from 16 countries in South Asia and the Middle East. India, Turkey, and Egypt had the highest rates of participation, followed by Pakistan, Iran, and Israel. The frequency of visits is influenced by political, economic, cultural, and institutional factors. But the most important factors appear to be foreign relations and geopolitics. Given the problems faced by national irrigation bureaucracies around the world, there is a need to focus more directly on political factors than in the past.     

早籼型水稻温敏核不育系M103S的选育与光温反应特性

以温敏核不育水稻安农S—1为母本，以常规高产早籼稻品系92153为父本进行杂交，通过低温选择和人工气候箱鉴定，育成了育性转换临界温度23—24℃的早籼型温敏核不育系M103S。光周期长于12．5h，日平均温度高于24℃，可以用于制种，日平均温度低于23℃，可以用于繁殖。光周期长于13．5h，日平均温度高于23℃，可以用于制种。自然条件下不育起点温度为日均温23—24℃，耐低温期为3—4d。敏感期为抽穗前的7—14d。播始历期为53—79d，主茎叶片数10．0—12．6片。从播种到始穗的活动积温和有效积温，分别为1493．8—1766．3℃和733．4—1058．3℃。不育期的株高63．6—77．5cm，平均72．0cm，单株有效穗6．4—12．0个，平均9．3个，穗长20．9—22．7cm，平均21．2cm，每穗颖花数112．1—137．3朵，平均122．3朵。可育期株高65．7cm，单株有效穗7．2个，穗长17．2cm，每穗总粒数114．1粒，结实率47．23％，千粒重31．34g。以M103S为母本，与100多个早籼品种进行测配，杂种表现生育期早、穗大、粒多、千粒重高。     

赛加羚羊的分子生物学鉴别

为了解决以往赛加羚羊(Saiga tatarica)鉴别方法存在的不确定性或分辨率低的问题,在mtDNA的12S rRNA基因易变区设计了2对赛加羚羊特异性引物Saiga A和Saiga B,通过PCR扩增和常规电泳检测,分别得到127 bp和320 bp的片段,进而可通过这两个片段的有无来特异性检出赛加羚羊的DNA。引物Saiga A和Saiga B的最小检出量为1．0 ng DNA,稳定性和特异性好、灵敏度很高,可以应用于赛加羚羊样品的检测。     

高通量测序解析冰鲜刀鲚细菌群落变化特征

为了研究养殖刀鲚冰鲜保存过程中细菌群落组成及变化情况,采用Illumina Miseq高通量测序方法对冰鲜条件不同贮藏时间（0、3、6、9、12、15、18 d）刀鲚肌肉样本细菌的16S rRNA基因的2个高变区（V3—V4）进行测序,比较其细菌多样性及门、属水平的细菌相对丰度,并进行主成分分析、Heatmap分析以及特定腐败菌分析。结果显示,7组样品细菌分布于40门787属,冰鲜条件下刀鲚肌肉的优势致腐败菌属为假单胞菌、微杆菌属和气单胞菌属。主成分分析显示,刀鲚腐败前后的微生物结构存在差异。Heatmap图分析显示新鲜样本的细菌丰度最大。3组冰鲜贮藏6 d前的样品群落结构较相似。贮藏12 d时常见致腐菌（微杆菌属、假单胞菌属、气单胞菌属）占比显著提高,其细菌的多样性及丰富度最低。随着冰鲜保存时间的延长,刀鲚肌肉的细菌菌落结构产生了动态变化。冰鲜保存6～9 d后刀鲚肌肉的细菌菌落结构变化较大,研究结果可以为预测冰鲜刀鲚货架期提供参考。     

A Novel Gelatinase from Marine Flocculibacter collagenilyticus SM1988: Characterization and Potential Application in Collagen Oligopeptide-Rich Hydrolysate Preparation

Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacterium Flocculibacter collagenilyticus SM1988^T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed in Escherichia coli showed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.     

水稻不育系培矮64S的空间诱变效应及后代的SSR分析

对经过卫星搭载的培矮64S种子及后代进行了有关性状研究和SSR标记分析,结果表明:培矮64S空间诱变后代SP1代发芽率、存苗率、株高、抽穗期、株叶型的性状与对照相比没有明显变化;但SP2代在株高、粒型和育性等性状上发生了变异;从SP3代中获得柱头比培矮64S明显增大的变异株系,这对于改良培矮64S的异交结实率和提高制种产量具有重要意义。此外,对10个SP3变异株进行SSR分析结果表明,扩增片段数目变化约占55.21%,扩增片段分子量变化约占44.79%,空间诱变引起的变异可能是以DNA缺失-重复为主。SSR座位的变异频率平均为23.33%,变异座位在水稻基因组中是随机分布的。