对虾白斑综合征病毒（WSSV）囊膜蛋白VP110部分重组表达及其与对虾鳃细胞膜蛋白结合分析

将VP110基因的部分序列克隆到pET-28a载体中构建pET28a-vp110b重组质粒并进行原核表达,获得重组表达的蛋白rVP110-B;用rVP110-B注射凡纳滨对虾Litopenaeus vannamei后,经WSSV感染,实验表明,该蛋白注射使凡纳滨对虾感染WSSV的半数死亡时间比对照组延长了20%。用表达纯化的该重组蛋白制备了兔抗rVP110-B多克隆抗体,该抗体用于凡纳滨对虾鳃细胞膜蛋白与rVP110-B的Far-western分析显示,凡纳滨对虾鳃细胞膜蛋白中除90 kDa左右的血蓝蛋白外,在41.7 kDa存在结合条带,经质谱分析表明这条鳃细胞膜蛋白是肌动蛋白。     

高效液相色谱法测定FQS-110的含量

用Ｉｒｒｅｇｕｌａｒ－Ｈ Ｃ１８色谱柱,以０．０５ｍｏｌ／ｌ柠檬酸溶液（以三乙胺调ｐＨ４．０）：乙腈（８１：１９,ｖ／ｖ）为流动相,以邻氨基苯甲酸为内标,于２７３ｎｍ波长处,测定ＦＱＳ－１１０含量,药物浓度（ｘ）与药物峰面积和内标峰面积之比（ｙ）呈良好的线性关系,线性方程为ｙ＝０．０８７５ｘ－０．０９３３,ｒ＝０．９９９４,线性范围为４￣４０μｇ／ｍｌ,日内与日间变异系数分别为０．６４％和０．６７     

FQS-11O对雏鸡人工感染鸡大肠杆菌的保护试验

用 FQS- 110对雏鸡人工感染鸡大肠杆菌进行了保护试验。于感染后 1、6、12、2 4 h给药 ,0 .335mg/ kg为 10 0 %保护率最小剂量 ,0 .16 0 mg/ kg的保护率为 90 %、0 .12 2 mg/ kg的保护率为50 %、0 .0 75mg/ kg的保护率为 10 %。经统计 ,ED50 95%可信限为 (0 .111± 0 .0 19) mg/ kg。试验结果提示 FQS- 110对雏鸡人工感染鸡大肠杆菌病有保护作用。     

Transgenic Expression of a Functional Fragment of Harpin Protein Hpa1 in Wheat Represses English Grain Aphid Infestation

The harpin protein Hpa1 produced by the rice bacterial blight pathogen promotes plant growth and induces plant resistance to pathogens and insect pests. The region of 10-42 residues （Hpa110-42） in the Hpa1 sequence is critical as the isolated Hpa110-42 fragment is 1.3-7.5-fold more effective than the full length in inducing plant growth and resistance. Here we report that transgenic expression of Hpa110-42 in wheat induces resistance to English grain aphid, a dominant species of wheat aphids. Hpa110-42-induced resistance is effective to inhibit the aphid behavior in plant preference at the initial colonization stage and repress aphid performances in the reproduction, nymph growth, and instar development on transgenic plants. The resistance characters are correlated with enhanced expression of defense-regulatory genes （EIN2, PP2-A, and GSL10） and consistent with induced expression of defense response genes （Hel, PDF1.2, PR-1b, and PR-2b）. As a result, aphid infestations are alleviated in transgenic plants. The level of Hpa110-42-induced resistance in regard to repression of aphid infestations is equivalent to the effect of chemical control provided by an insecticide. These results suggested that the defensive role of Hpa110-42 can be integrated into breeding germplasm of the agriculturally signiifcant crop with a great potential of the agricultural application.     

双流县农牧110的建立与实践

为创新服务机制,强化服务职能,提高服务水平,体贴农民,关心农民,帮助农民,切实为农民办实事,在双流县委、县政府的关心支持下,成立了“双流县农牧110”。经过一段时间的宣传和运行,已得到了农民群众的认可,取得了初步的成效。本文从农牧110的建立、农牧110的运行、几点体会、努力方向等方面入手,介绍了农牧110从成立到现在的运行情况,具体做法和取得的主要成效。提出了农牧110今后的努力方向,要把农牧110建成为农民群众服务的民心工程,要以农牧110建设为契机,加快构建和完善农村服务体系,助推社会主义新农村建设。     

不同遗传背景猪PBD-110基因多态性及表达谱分析

为探究猪β-防御素110（PBD-110）基因多态性及其在猪组织中的表达情况,本试验以野杂猪、民猪和大白猪为研究对象,分别扩增PBD-110基因编码区（CDS）和内含子区,采用实时荧光定量PCR法探究3个群体猪肝脏和脾脏的组织表达差异。试验成功扩增出猪PBD-110基因CDS区和内含子区,测序证实3个群体猪PBD-110基因CDS区不存在突变位点,基因组PCR产物测序发现在内含子1存在C314T突变位点,3个群体多态性分析发现CC为优势基因型,基因型频率依次为0.750、0.781和0.516。Hardy-Weinberg平衡检验同时发现该位点在3个群体中均处于平衡状态（P>0.05）,且处于中、低多态性状态（0.25 < PIC < 0.5;PIC < 0.25）。表达谱分析结果表明,PBD-110基因在3个群体猪肝脏和脾脏中均有表达,且存在表达差异,提示PBD-110基因可能与民猪、野杂猪抗病力较强有关,在天然免疫方面发挥重要作用。     

大豆垄上三行“大垄密”栽培技术

介绍大豆110 cm垄上三行"大垄密"栽培技术,该技术是一项垄平结合、宽窄结合、旱涝综防的大豆栽培模式。主要解决阶段性降雨多或是土壤库容小,不能存放多余水的大豆栽培技术。在旱年比70 cm的三垄栽培能增产10%以上,在涝年比其他的大垄密栽培技术增产6%以上,常年其大豆产量能稳定保持在3 000 kg/hm2以上。     

高温高压下P110钢CO2腐蚀电化学行为研究

运用电化学测量技术,研究了P110油套管钢在模拟油田现场腐蚀环境中的腐蚀电化学行为.研究结果表明,随着试验时间的延长,腐蚀速率逐渐降低.在试验时间达到72 h后,腐蚀速率降低的趋势变缓;阳极极化曲线上出现了类似于钝化的现象.阴极极化曲线的Tafel斜率变化较大,P110钢的阴极反应趋势已经不同;P110钢的EIS表明极化电阻逐渐增大,电极反应由最初的活化控制变为由扩散和活化共同控制.     

Variability at the electron microscopic level in leaves of members of the genus Vitis

Scanning electron microscopy (SEM) was used to examine the characteristics of the upper and lower sides of leaves belonging to members of the genus Vitis – 11 vinifera varieties (‘Albariño’, ‘Treixadura’, ‘Caíño Blanco’, ‘Mencía’, ‘Chasselas’, ‘Cabernet Sauvignon’, ‘Alicante Bouschet’, ‘Godello’, ‘Torrontés’, ‘Blanco Legítimo’ and ‘Caíño Tinto’) and three non-vinifera varieties (‘110-Ritcher’, ‘SO4’ and ‘Jacquez’). All the genotypes studied grew in the same plot, were of the same age, and had been raised following the same cultivation practices. The leaves examined were taken from node 8 of a fruiting shoot growing from the previous year's wood. The same part of the leaf – the area between the main vein and the first right lateral vein – was examined in all plants. SEM observations were made at 200×, 655× and 2000×. Photomicrographs and drawings were prepared reflecting the observed morphology of the upper and lower leaf surfaces. Variables such as cell density, cell surface area (in the horizontal plane) and stomatal density and surface area (in the horizontal plane) were measured for both leaf surfaces, and intra- and inter-varietal differences recorded. The upper leaf surfaces of all the studied genotypes were similar, but clear differences were recorded for the lower sides.     

农业科技110综述

农业科技110作为全国三大农业科技推广模式之一,被广大农民誉为传送科技信息的“及时雨”、帮助农民致富的“好帮手”、实现产供销结合的“好桥梁”,本文就有关农业科技110进行综述。