胆固醇转运蛋白NPC1L1的研究进展

NPC1L1是近年来人们研究高脂血症的重点内容,该蛋白已被证实在胆固醇的肠道吸收和胆汁分泌中发挥了关键作用。NPC1L1调节体内胆固醇的生物合成,是维持生物体胆固醇动态平衡的重要因素,同时也是新型降脂药物依泽替米贝的作用靶点。     

我国粮食主产区秸秆资源量估算与利用

2011年国家发改委、农业部、财政部联合印发了《十二五农作物秸秆综合利用实施方案》,提出在十二五期间在13个粮食主产区等重点区域开展秸秆综合利用技术。本文通过选取各省区分类的秸秆系数对各粮食主产区近年来的秸秆资源量进行估算,并对粮食主产区的秸秆利用的现状和问题进行分析。一、秸秆资源量估算存在的问题我国是农业生产大国,有着丰富的作物秸秆,随着国家对作物秸秆资源综合利用的重视,秸秆资源量也将逐渐成为农业生产的产量目标。目前对于秸秆资源量的估算一般存在以     

Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning

The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H₂O),cadmium chloride group (2.3 mg/kg CdCl₂),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl₂),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.     

罹患口腔炎乌苏里蝮的相关生理学指标分析

口腔炎是人工养殖蛇类的常见病之一,为了深入了解和尽早做好预防工作,对罹患口腔炎的成年雄蛇乌苏里蝮(Gloydius ussuriensis)进行解剖观察,并检测了相关器官的系数、血液学指标和激素等。结果表明:整体肥满度、躯体肥满度、肾系数等数值罹患口腔炎蛇略高于健康蛇;而肝系数、精巢系数和脂肪体系数等罹患口腔炎的蛇低于健康雄蛇,且前两者呈显著性差异,后者呈极显著性差异。性激素检测结果显示罹患口腔炎的蛇血液睾酮和雌二醇含量均低于健康蛇,且均具极显著性差异。血细胞指标中,红细胞数量、红细胞分布均差、白细胞总数、淋巴细胞数量和淋巴细胞比率等均为罹患口腔炎的蛇高于健康雄蛇,且具显著性差异,但嗜中性粒细胞和淋巴细胞的比值(NLR)、中性粒细胞数量和比率、中间细胞数量及其比率均为患口腔炎的雄蛇低于健康蛇,且具显著性差异。结论:口腔炎不仅影响了主要代谢器官的器官系数的变化,而且血液多项指标和性激素水平也有明显差异。性激素含量的变化将对乌苏里蝮的正常生殖繁育产生不可忽视的影响。另外,NLR和红细胞分布宽度2个血常规指标可作为早期诊断炎症发生的首选参数。     

马、骡蹄角质某些物理性能的测定

对马(20)、骡(10)蹄角质的测定结果表明,硬度、弹性,抗张强度、弹性模量均为蹄壁>蹄底>蹄叉;蹄底的磨耗大于蹄壁;蹄壁的磨擦系数大于蹄底;蹄壁纵向拉伸的抗张强度大于其横向拉伸,强度越大其伸长率越小;在相同压力下,蹄壁侧压弹性模量值大于纵压值,而蹄底侧压模量值则小于纵压值;蹄角质在材料力学上表现为一种各向异性的特点。作者认为,马、骡蹄角质物理性能的差异与蹄病的发生密切相关。     

Caco-2细胞模型及其在营养素小肠吸收机理研究中的应用

Caco-2细胞源自人结肠癌细胞,体外培养时能自发地进行类似肠道细胞的形态学和生化学上的分化,获得许多小肠吸收细胞的特性,如形成微绒毛结构;在细胞表面形成良好的刷状缘;在细胞间形成紧密连接;分泌水解酶以及合成转运糖、氨基酸和药物等的载体转运系统。由培养在微孔滤膜上的Caco-2细胞构建的模型为研究营养素在小肠的吸收机理提供了一个有效且易于操作的实验手段。本文主要综述了Caco-2细胞模型的建立、特征、检测及其在氨基酸、维生素、核苷和微量元素等营养素小肠吸收机理研究中的应用。     

我的中蜂转运经历及"全封闭中蜂运输"法

中蜂要进行产业化发展,首先要解决中蜂的运输问题.而中蜂运输与意蜂运输确实有较大差异.一般来说,中蜂蜂群对转运较难适应,人们普遍认为,中蜂只适宜小转地或定地饲养,能否适应转运,如何转运才安全?我认为还应由客观事实来证明.     

中国地方猪种间的核型似近系数

计算了１６个中国地方猪种的核型似近系数，采用最短距离法进行了系统聚类。结果表明，各猪种间的核型似近系数在０．８９１３—０．９８７８之间，反映出中国猪种在核型上的高度稳定性和高度相似性。在核型上，华中型猪种更倾向于江海型；华南型更接近于西南型。