五种"种植+小规模养猪"模式

为了引导农户充分利用土地和庭院资源优势,积累发展资金,根据对农户养猪生产的现状分析,现提出五种"种植 小规模养猪"的技术模式,供参考.     

杜仲果实内杜仲胶形成积累规律的研究

采用选择典型样株和随机取样的方法对杜仲果实含胶率的年变化及逐年变化特点和规律进行了研究。结果表明,杜仲果实内含胶特性的年变化特点可以划分为两个阶段：在果实基本停止生长以前,果皮和果实含胶率变化与果实的生长发育密切相关,果皮和果实含皎率随着果实的生长而迅速提高;在果实基本停止生长以后,含胶率提高缓慢。不同树龄杜仲果皮和果实的含胶率比较稳定。采用高接换雌建园和嫁接苗建园两种方式建立杜仲高产皎果园都具有十分明显的增产效果。     

不结球白菜高亲和性硝酸盐转运蛋白NRT2.2的克隆和表达分析

以不结球白菜品种"苏州青"为材料,克隆得到了1个高亲和性硝酸盐转运蛋白基因——BcNRT2.2,该基因的全长为1593 bp,编码530个氨基酸。序列分析结果表明:BcNRT2.2基因含有AGWGNMG共识别基序,其氨基酸序列与拟南芥NRT2.2的同源性为86%;其二级结构主要由α-螺旋和自由卷曲构成;预测BcNRT2.2蛋白含有12个跨膜域。实时定量PCR分析结果表明:BcNRT2.2主要在根部表达,并受高浓度硝酸盐的诱导;BcNRT2.2在原始叶片中表达量较低,但受低温、高温和干旱胁迫的诱导后而高量表达。     

高寒地区燕麦籽粒糖降解和淀粉积累动态分析

为探讨高寒地区燕麦花后籽粒中糖降解和淀粉积累特点,分析了2个品种花后籽粒中糖降解和淀粉积累动态。结果表明籽粒中淀粉均呈"S"型曲线增长,花后6~15d可溶性糖转化速率较高,淀粉积累速率较快,其后可溶性糖转化速率和淀粉积累速率较低,可溶性糖处于动态平衡中。     

妊娠母猪生产异常的原因及防治

本人从事基层畜牧兽医工作近20年,对妊娠母猪的异常生产（早产、流产、死胎、畸形胎、木乃伊等）多次接诊,并对成因和防治措施有一定的经验积累,现报告如下：     

Effect of diacylglycerol acyltransferase 2overexpression in 3T3-L1 is associated to an increase in mono-unsaturated fatty acid accumulation

Background：Fatty acid（FA） composition is the most important parameter affecting the flavor and nutritional value of the meat.The final and the only committed step in the biosynthesis of triglycerides is catalyzed by diacylglycerol acyltransferase 2（DGAT2）.The role of DGAT2 in lipid accumulation has been demonstrated in adipocytes,However,little is known about the effect of DGAT2 on the FA composition of these cells.Methods：To investigate the role of DGAT2 in regulating lipid accumulation,FA composition and the expression of adipogenic genes,we cloned the open reading frame of the porcine DGAT2 gene and established 3T3-L1 cells that overexpressed DGAT2.Cells were then cultured in differentiation medium（DM） without FA,with a mixture of FAs（FA-DM）,or containing a ~（13）C stable isotope-labeled FA mixture（IFA-DM）.The FA composition of adipocytes was analyzed by gas chromatography-mass spectrometry and gas chromatography-isotope ratio mass spectrometry.Quantitative PCR and western blotting were employed to detect expression of adipogenic genes in 3T3-L1 adipocytes cultured with FA-DM for 12 d.Results：The triacylglyceride（TAG） content was significantly higher in 3T3-L1 adipocytes overexpressing DGAT2 than in control cells.When cultured in DM or FA-DM for 12 d,cells overexpressing DGAT2 showed a higher proportion of unsaturated FAs（C16：1 and C18：1）.However,when cells overexpressing DGAT2 were cultured with FA-DM for30 min,the FA composition was almost identical to that of controls.Further,the proportion of stable isotope-labeled FAs were similar in 3T3-L1 adipocytes overexpressing DGAT2 and control cells cultured in IFA-DM for 12 d.These results collectively indicate that the higher proportion of mono-unsaturated FAs,C16：1 and C18：1,may originate from de novo FA synthesis but not from the uptake of specific FAs from the medium.This hypothesis is further supported by evidence that both mRNA and protein expression of genes involved in FA synthesis（ACACA,FASN,SCD1,and A-FABP?     

氮肥施用量对辽东栎幼苗生长和氮代谢相关酶活性的影响

在田间条件下,设置0 g/株(对照)、4 g/株、8 g/株、12 g/株、16 g/株5个氮肥施用量处理,研究不同氮肥施用量对辽东栎幼苗生长和氮代谢相关酶活性的影响。结果表明,辽东栎的总干物质积累量随着氮肥施用量的增加而增加,表明施用氮肥对植物生长具有显著的促进作用。施用氮肥提高了辽东栎的硝酸还原酶活性、谷氨酰胺合成酶活性、谷氨酸脱氢酶活性、谷氨酸丙酮酸转氨酶活性和可溶性蛋白含量,因此,施用氮肥提高了辽东栎的氮代谢相关酶活性及其氮吸收和存储能力,从而促进了辽东栎生长,提高了各器官干物质积累量。不同处理比较来看,12 g/株是辽东栎育苗实践中适宜的氮肥施用量。