EMS和60^Co-γ射线辐照复合诱变黄麻突变体苗期生理生化特性研究

为丰富黄麻种质资源和扩大突变体容量,本研究采用物理诱变（60^Co-γ）和化学诱变（EMS）相结合的方法,以＂中黄麻4号＂等7个黄麻品种为材料,观察记录诱变黄麻苗期的形态学变化,分析诱变黄麻的代谢特性和抗性指标。结果表明EMS和60^Co-γ射线对黄麻幼苗的叶片产生不同程度的损伤,前者主要导致叶片卷曲,后者导致叶片分叉。复合诱变导致黄麻幼苗脯氨酸含量、丙二醛含量和根系活力提高。而可溶性蛋白、SOD活性和POD活性变化则品种之间呈现差异。本研究为黄麻种质创新的方法提供了参考,并且丰富了黄麻突变体的材料。     

利用响应曲面法优化γ-亚麻酸-β-环糊精包合物制备工艺的研究

[目的]优选β-环糊精包合γ-亚麻酸的最佳工艺。[方法]采用BOX-Behnken设计和响应曲面分析优选包合的最佳工艺,并采用差示扫描量热分析及X-射线衍射对包合物进行定性分析。[结果]β-环糊精包合γ-亚麻酸的最佳工艺条件为：β-环糊精与γ-亚麻酸的物质的量比6.47∶1、温度51.15℃、包合时间2.03h,在该条件下包合率达91.51%。定性分析表明γ-亚麻酸与β-环糊精确实形成了包合物,其包合物的结构与简单混合物的结构有明显不同,已构成新的固体相。[结论]该研究可有效地减少工艺操作的盲目性,为进一步的放大生产提供依据。     

东北虎γ-干扰素基因的克隆、生物信息学分析及其在毕赤酵母中的表达

本试验旨在通过克隆东北虎γ-干扰素(IFN-γ)基因,研究其分子特征并预测蛋白生物学功能,为后续研究干扰素抗病毒活性做前期准备。通过RT-PCR从ConA诱导过的东北虎血淋巴细胞中扩增东北虎IFN-γ基因并测序,应用生物信息学方法进行序列分析。结果表明:东北虎IFN-γ编码区由504个核苷酸组成,共编码167个氨基酸,蛋白相对分子质量为19.59 ku,等电点为9.03,所编码的蛋白为碱性亲水性蛋白,其中前23个氨基酸可能为信号肽,IFN-γ编码蛋白保守结构域为IFN-γ超家族,且存在跨膜结构,其中1-6位氨基酸为胞内区域,7-28位氨基酸为跨膜区域,29-167位氨基酸为胞外区域;IFN-γ编码蛋白二级结构主要以α-螺旋(58.08%)和无规则卷曲(33.53%)为主,存在5个潜在的B细胞抗原表位,3个潜在的N-糖基化位点;分子进化分析显示,东北虎IFN-γ与GenBank上发表的东北虎、非洲狮、金钱豹、美洲狮、猎豹、家猫、加拿大猞猁、野猪等的核苷酸相似性为80.4%~99.8%,氨基酸相似性为70.5%~100%,东北虎IFN-γ与非洲狮、金钱豹亲缘关系最近,美洲狮、猎豹、家猫、猞猁次之,野猪最远。通过合成改造后的东北虎IFN-γ基因,构建能表达IFN-γ蛋白的重组质粒pPIC9K-IFN-γ,将其导入高效表达系统-毕赤酵母中进行诱导表达,经SDS-PAGE分析,表达蛋白的分子量约17.8 ku,与预期大小相符,表明东北虎IFN-γ成功表达。     

Immunohistochemical expression of interferon-γ in different types of granulomatous lesions associated with bovine paratuberculosis

Animals infected with Mycobacterium avium subsp. paratuberculosis (Map) show a variety of lesions, from focal forms, seen in subclinical stages to diffuse lesions in clinical cases. The purpose of this study was to evaluate the local expression of IFN-γ by immunohistochemistry in relation with the type of lesion in naturally Map-infected cows. The number of immunolabelled cells, −the majority morphologically consistent with lymphocytes-, was higher in focal and diffuse paucibacillary forms than in diffuse multibacillary lesions, where they appeared closely related to epithelioid cells. Diffuse multibacillary lesions had the lowest numbers, but higher than controls, and positive cells were intermingled among the macrophages. The peripheral IFN-γ production was higher in all Map infected cows and a positive correlation was found with the number of immunolabelled cells in the intestine. The findings of this study show that IFN-γ would play a role in the development of the different types of lesions in paratuberculosis, and also points out the importance of adequate sampling of lymphoid tissue containing samples when studying the local immune response in which IFN-γ expression may be involved, especially in cases where focal lesions are present.     

以松香基季铵盐为模板剂合成介孔γ-Al2O3（英文）

以松香基季铵盐为结构导向剂制备均匀介孔氧化铝,采用XRD,N2-吸附脱附和TEM表征样品的结构性能.结果表明:经过650℃焙烧,所得样品为介孔γ-Al2O3,比表面积为194～213 m2/g,平均孔径为4.18-5.18 nm.松香基季铵盐的添加量对氧化铝的结构性能有着较大的影响,适量添加松香基季铵盐有利于氧化铝获得较大的孔径和较窄的孔径分布.     

Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis

IntroductionCystic echinococcosis (CE) is a chronic zoonotic disease caused by the larval stage of Echinococcus granulosus (E. granulosus), which affects domestic and wild carnivores as the definitive host and ungulates as intermediate hosts. In intermediate hosts, both Th1 and Th2 cells are involved in the immune responses to an echinoccocal infection. This study aimed to investigate production of IL-4, IL-10, and IFN-γ cytokines in peripheral blood mononuclear cells (PBMCs) of CE patients before and after surgical treatment.MethodsTo evaluate cytokine production in response to E. granulosus antigens, we investigated IL-4, IL-10, and IFN-γ production in PBMCs of 20 CE patients in response to hydatid cyst fluid antigen (HCF-Ag) before and after surgical treatment using ELISA.ResultsThe mean IL-4 production from HCF-Ag stimulated PBMCs was significantly decreased (p < 0.05), while IFN-γ was significantly increased in HCF-Ag stimulated PBMCs in patients after surgery (p = 0.005).Furthermore, our results showed that there is no significant difference between IL-10 production in patients before and after treatment (p = 0.562).ConclusionsOur data Indicated production of IL-4 in cultured PBMCs of CE patients stimulated with HCF-Ag was decreased significantly. While, production of IFN-γ was increased significantly in responses to HCF Ag after surgery. We concluded that the evaluation of IL-4 and IFN-γ in HCF-Ag stimulated PBMCs of CE patients should be considered as a useful marker in the follow up of patients with cystic echinococcosis.     

Spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacenta for regulating of placental angiogenesis through VEGF-mediated signalling

Non-infectious prenatal mortality severely affects the porcine industry, with pathological placentation as a likely key reason. Previous studies have demonstrated that peroxisome proliferator-activated receptor gamma (PPARγ) deficiency causes defects in the uteroplacental vasculature and induces embryonic losses in mice. However, its role in porcine placental angiogenesis remains unclear. In the present study, PPARγ expression was investigated in porcine uteroplacental tissues at gestational day (GD) 25, GD40 and GD70 via quantitative polymerase chain reaction (qPCR), Western blot and immunohistochemistry (IHC). Moreover, the roles of PPARγ in porcine placental angiogenesis were investigated using a cell model of porcine umbilical vein endothelial cells (PUVECs) to conduct proliferation, migration and tube formation assays in vitro and a mouse xenograft model to assess capillary formation in vivo. The results showed that PPARγ was mainly located in the glandular epithelium, trophoblast, amniotic chorion epithelium and vascular endothelium, as indicated by the higher expression levels at GD25 and GD40 than at GD70 in endometrium and by higher expression levels at GD40 and GD70 than at GD25 in placenta. Moreover, PPARγ expression was significantly downregulated in placenta with dead foetus. In PUVECs, knocking out PPARγ significantly inhibited proliferation, migration and tube formation in vitro and inhibited capillary formation in mouse xenografts in vivo by blocking S-phase, promoting apoptosis and downregulating the angiogenic factors of VEGF and its receptors. Overall, the spatiotemporal heterogeneity of PPARγ expression in porcine uteroplacental tissue suggests its vital role in endometrial remodelling and placental angiogenesis, and PPARγ regulates placental angiogenesis through VEGF-mediated signalling.     

木材光引发自由基规律的研究

木材在~(60)Co γ射线辐射下引发g值为2.019、2.029、2.027、2.016、1.999等几种自由基。ESR主谱自由基浓度远大于机械自由基。并与树种、辐射剂量等有关。主谱自由基相对强度随剂量x按指数率Y=1-e~(-ax)上升,当剂量增加到100KGy时趋于饱和,根据福建21个常见树种测试有明显的规律性。     

富γ-氨基丁酸茶的药理功能研究进展

综述了富γ-氨基丁酸茶的药理功能和γ-氨基丁酸富集方法的研究进展,提出应进一步改进和完善富γ-氨基丁酸茶的加工工艺和我国富γ-氨基丁酸茶叶产品的广阔市场前景。     

包膜材料γ-聚谷氨酸对菜心的农学效应

【目的】考察施用γ-聚谷氨酸（γ-PGA）对菜心生长、产量、品质和养分吸收的影响,揭示γ-PGA的农学施用效应。【方法】在酸性土壤（pH 5.89）和强酸性土壤（pH 4.85）以菜心为供试作物进行盆栽试验。在常规施肥（施N 1.33 g/kg土,分5次施入土壤）的基础上,设不施γ-PGA包裹型肥料（T1）、分5次施用γ-PGA 0.53 mg/kg土（T2）和1.06 mg/kg（T3）、在定苗一周后一次性淋施γ-PGA 0.53 mg/kg土（T4）和1.06 mg/kg土（T5）5个处理。调查了菜心根系活力、养分含量、养分吸收累积量、叶片抗氧化系统、菜心产量及品质。【结果】酸性、强酸性土壤上,γ-PGA处理（T2、T3、T4、T5）的伤流强度均高于T1,且T3、T4、T5的伤流液氨基酸输出强度显著高于T1,平均增幅分别达到38.1%、33.2%、44.2%（P < 0.05）。酸性土壤上,T2、T3、T4、T5处理的菜心钙、镁含量显著高于T1（P < 0.05）,菜心钙的增幅分别为29.6%、21.8%、26.4%、22.7%,镁的增幅分别为11.7%、8.3%、22.2%、16.7%,锌、锰也有一定程度的提高;强酸性土壤上不同处理的菜心养分含量没有差异。T2、T3、T4、T5处理的氮、磷、钾、钙、镁、铜、锌、铁、锰累积量均显著高于T1;T4、T5处理的菜心叶片可溶性蛋白显著高于T1（P < 0.05）,T2、T3、T4、T5处理的菜心叶片CAT活性显著高于T1（P < 0.05）;而酸性土壤上,仅T3处理的菜心叶片CAT活性显著高于T1处理（P < 0.05）。酸性、强酸性土壤上,T3、T4处理的菜心叶片SOD活性显著高于T1（P < 0.05）;不同处理的菜心叶片POD活性没有差异;T2、T3、T4、T5处理的菜心产量均显著高于T1（P < 0.05）,在酸性土壤上增幅分别为4.4%、7.3%、12.2%、12.3%,强酸性土壤上分别为9.7%、14.2%、12.2%、12.39%。两种类型土壤上,T2、T3、T4、T5处理的菜心地上部分和根系生物量均显著高于T1处理（P < 0.05）。T4、T5处理的Vc含量和游离氨基酸含量显著高于T1（P < 0.05）,T2、T3、T4、T5处理的硝酸盐含量显著低于T1（P < 0.05）。γ-PGA淋施处理（T4、T5）的菜心根系活力、养分含量、养分累积量、叶片抗氧化能力、产量和品质均优于γ-PGA包裹肥料施用处理（T2、T3）。不同γ-PGA用量水平不影响菜心产量、品质、养分含量及叶片抗氧化能力。在酸性土壤上,γ-PGA对菜心养分吸收累积影响更为显著,而在强酸性土壤上,γ-PGA对菜心叶片抗氧化能力影响更突出。【结论】施用γ-PGA可提高菜心根系活力,促进菜心对养分,尤其是中微量元素的吸收,并增强菜心抗逆性,产量显著提高,一次施用效果好于分多次施用。