迷宫流道转角对灌水器水力性能的影

为研究齿形、梯形以及矩形流道转角变化对水力性能的影响,采用Fluent软件对不同形状下不同转角的流道进行了数值模拟.研究结果表明:当其他条件相同时,转角的变化与流量系数、流态指数呈负相关,其变化对梯形流道灌水器的流量系数影响最大,最多下降了19.03%,齿形流道次之,下降了10.14%,矩形流道是梯形流道转角角度增加的延伸,具有相同的水力性能变化规律;随着角度的增加,梯形流道总的局部水头损失系数最多增加了32.5%,而齿形流道总的局部水头损失系数最多增加了23.4%,变化都很明显;压力较高时,摩阻系数基本保持不变,流体为紊流状态.     

不确定性条件下分布式农业生产预警模型构建与应用

构建了基于梯形模糊数和分布式作物模拟模型的空间分布式农业生产预警模型来实现作物产量和水分生产力综合警情预警预报。模型采用空间分布式作物产量和水分生产力作为警情指标来计算系统警级,引入梯形模糊数来表征目标产量和水分生产力的不确定性,采用空间分布式作物模拟模型来模拟常规灌溉、0.8倍常规灌溉和0.6倍常规灌溉下的作物产量和水分生产力,进而对现状1976—2012年和未来RCP4.5情景下2026—2045年不同灌溉水平下进行农业生产风险预报预警,并衡量了未来20年产量和水分生产力的静态协调度和每5年4个周期的动态协调度。结果表明,同一作物在不同土壤类型和不同灌溉水平下预警等级不同,警级随着灌溉水平的降低呈现不规则变化规律,协调性随着灌溉水平的降低而减小。模型能够识别出未来气候变化不同节水灌溉水平下的空间异质性作物产量和水分生产力的警级,实现精准化农业生产风险预警预报,有利于实现高效率降警处理。     

Effect of membrane environment at high temperature on delayed rectifier K+ channel in hypothalamus neurons

AIM: To observe the effect of high temperature on the delayed rectifier K⁺ channel(I_K) in hypothalamus neurons METHODS: The kinetic properties of I K were measured by cell-attached mode of patche-clamp technique RESULTS: The conductance, long-term open constant(τ _o2) and open probability(Po) of I_K increased with increase of temperature (P <0.05), while more burst open and multi-order open were recorded Many types of currents occurred after heat stress at 43℃,and voltage-dependent property reduced and even depressed CONCLUSIONS: The activating action of I_K might be affected obviously by high temperature, the coupling between I K and body fever or heatstroke remained to be established.     

Cardiac L-type calcium channel and its alterations in cardiac hypertrophy and heart failure

There are two type of cardiac calcium channel current, both are inward current: (1) L-type Ca²⁺channel current (I_ca-L), plays a major role in determining myocyte action potential plateau characteristics as well as initiating the myocyte excitation-contraction coupling; (2) T-type Ca²⁺ channel current (I_ca-T), probably plays an important role in pacemaker activity. The alterations of L-type calcium channel abundance and function in cardiac hypertrophy and heart failure are determined by the the species difference, especially by the stage of the disease process and the degree of the disease. Both abundance and function of L-type calcium channel decrease in severe hypertrophy and heart failure.     

Mechanism of quercetin improving rat coronary artery myogenic response under high glucose

AIM: To investigate the mechanism of quercetin improving rat coronary artery myogenic response under high glucose (HG) by measuring muscle tension of coronary arterial ring and recording voltage-gated K⁺ channel (Kv) current of coronary artery smooth muscle cells by whole cell patch clamp. METHODS: The coronary rings from the normal SD rats were acutely isolated, and then divided into 6 groups: (1) control group; (2) HG group; (3) HG+low dose (3 μmol/L) of quercetin group; (4) HG+moderate dose (10 μmol/L) of quercetin group; (5) HG+high dose (30 μmol/L) of quercetin group; (6) HG+C6303 (PKC inhibitor)+high dose of quercetin group. Determinations of coronary artery response to vasoconstrictor (60 mmol/L KCl or 0.1 mmol/L U46619) or vasodilator (ACh at 10^-9~10^-5 mol/L) were performed, and the percentage of coronary ring tension was calculated using the contraction as 100% caused by 60 mmol/L KCl. The rat coronary artery smooth muscle cells were acutely isolated for recording the Kv current using whole cell patch clamp. RESULTS: Compared with control group, the contraction amplitudes to 60 mmol/L KCl or 0.1 mmol/L U46619 were significantly increased under HG incubation. Quercetin intervention concentration-dependently reduced the coronary artery contraction amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, the diastolic amplitude to ACh decreased significantly in HG group, and quercetin intervention concentration-dependently increased the coronary artery diastolic amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, HG incubation inhibited Kv current of coronary artery vascular smooth muscle cells significantly, and quercetin intervention attenuated the inhibitory effect of HG on Kv current intensity. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. CONCLUSION: Quercetin has a protective effect on myogenic response of coronary artery under HG and the effects is related to the increase in Kv current and the activation of PKC in vascular smooth muscle cells.     

Farrerol inhibits nicotine-induced proliferation of pulmonary vascular smooth muscle cells by enhancing Kv1.5 and Kv2.1 expression

AIM:To study the effect of farrerol (Far) on nicotine-induced proliferation of rat pulmonary smooth muscle cells (PASMCs), and further to explore its relationship with voltage-dependent potassium channels (Kv) 1.5 and Kv2.1. METHODS:Firstly, the effect of nicotine on the proliferation of PASMCs was detected by cell counting method, and the optimal concentration of nicotine was selected. Primary cultured PASMCs were randomly divided into 5 groups:normal control group, nicotine (1 μmol/L)group, nicotine (1 μmol/L) + Far (10^-6 mol/L, 10^-5 mol/L and 10^-4 mol/L) Far group. The activity of caspase-3 was measured by apoptosis kit, the cell viability was measured by CCK-8 assay, the apoptotic rate was analyzed by flow cytometry. The expression of Kv1.5 and Kv2.1, and apoptosis-related factors Bcl-2 and Bax at mRNA and protein levels was determined by RT-qPCR and Western blot respectively. RESULTS:Nicotine at 1 μmol/L increased the number of PASMCs to the maximum extent (P<0.01). Nicotine at 1 μmol/L significantly reduced the caspase-3 activity and enhanced the cell viability of the PASMCs (P<0.01). Farrerol at 10^-6~10^-4 mol/L eliminated the effect of PASMCs induced by nicotine in a concentration dependent manner. Compared with control group, nicotine at 1 μmol/L significantly increased the proliferation and inhibited the apoptotic rate of rat PASMCs (P<0.01). The apoptotic rate of PASMCs in farrerol intervention group was significantly higher than that in nicotine group (P<0.01). Nicotine at 1 μmol/L significantly inhibited the expression of Kv1.5, Kv2.1 and Bax but increased the expression of Bcl-2 in PASMCs (P<0.01). Farrerol at 10^-5 mol/L obviously inhibited the effect of PASMCs induced by nicotine. CONCLUSION:Farrerol eliminates nicotine-induced inhibition of caspase-3 and Bax, and enhancement of Bcl-2 in PASMCs by enhancing Kv1.5 and Kv2.1 expression.     

典型断面渠道临界水深计算

系统总结了明渠特征水深研究领域的计算方法,评价了各方法的特点;为了优选出典型断面渠道临界水深简捷、通用、精度高、适用范围广的显式计算公式,通过定义包含典型断面几何要素及流量的量纲为一的参数,将目前成果中临界水深的显式计算公式用定义量纲为一的参数进行统一表达,并对其进行简捷性、精度及适用范围的综合评价比较,优选出梯形、圆形、弧底梯形、普通城门洞形、马蹄形等5种典型断面渠道临界水深的显式计算公式;对标准城门洞形断面的临界水深应用最优一致逼近原理,提出以幂函数形式分段表达的新显式计算公式．误差分析表明,在工程常用范围内,由推荐的显式公式所计算的6种典型断面的临界水深,其最大相对误差均小于1％,满足工程设计对精度的要求．该研究可为典型断面排灌渠道的设计及水力计算提供参考．     

水面蒸发对南水北调江苏段调水效率的影响

为了掌握南水北调东线江苏境内工程输水河道和调蓄湖库水面蒸发损失水量,及其所造成的泵站机组损失功率,了解水面蒸发对工程调水效率的影响,分析比较了适用于工程所在地区的水面蒸发量计算模型和计算公式,计算了工程沿线输水河道和调蓄湖库的水面蒸发量,分析了工程水面蒸发量的时间和空间分布特征.通过积分,计算了水面蒸发造成的各梯级泵站的流量损失和输入功率损失,求解并分析了水面蒸发对整个江苏段工程调水效率的影响.结果表明：工程沿线水面年蒸发量自南向北、西北方向逐渐递增;蒸发量夏季最高,冬季最低;全线平均水面年蒸发量为862.2 mm.洪泽湖、骆马湖、白马湖和输水河道蒸发损失水量分别占总蒸发损失水量的77.7%,13.3%,3.6%和5.4%,蒸发损失水量约占源头泵站抽水量的17.46%,使工程平均调水效率降低10.33%.调水工程可以考虑采用管道输水,从而避免蒸发损失.     

蒋益兰教授从脏腑经络辨证论治乳腺癌经验探析

蒋益兰教授为湖南省名中医,从事中西医结合防治肿瘤的临床、科研工作30余年,认为乳腺癌的发生发展与肝郁、脾虚、肾亏、冲任失和密切相关,主要病理因素为痰、瘀、毒,从平调肝气、健脾化痰、补肾滋阴、调补冲任出发,以四君子汤、逍遥散、二至丸等方药加减,佐以白花蛇舌草、半枝莲、重楼、莪术、鸡血藤、土贝母、夏枯草、猫抓草、生牡蛎、山慈菇等治疗乳腺癌。     

花生生产潜力与高产途径

据估算，中熟大花生和早熟小花生最高单产可达到17.3t/hm^2和11.9t/hm^2，实际生产中，中熟大花生和早熟小花生最高单产已达到11.2t/hm^2和9.5t/hm^2。目前全球花生平均单产不到1.0t/hm^2，我国平均约3.7t/hm^2，花生生产仍有较大的增产余地。延长作物生长期、提高植株光合效率和增加光合产物向生殖体的分配比例，是花生品种改良的有效途径。适期播种、创造良好的土壤环境、选择适宜的种植方式和密度、合理调控叶面积系数、在增加总生物产量的基础上适当提高经济系数等，是目前花生高产栽培有效的措施途径。