曼地亚红豆杉生物学特性研究

介绍了曼地亚红豆杉的分类地位及形态、生理、生化、组培、繁殖等生物学方面的特性.     

滤膜过滤器新的理论模型初步探讨

通过对滤膜过滤器的过滤机理进行分析，认为过滤器在使用期间，滤膜中每个毛细管的过滤性能随时间和空间位置的变化而变化，因而对过滤器性能的评价还应从系统的、全面的、宏观统计的观点来综合反映，由此可根据多孔介质流体动力学理论模型来准确地反映滤膜过滤器的性能变化。     

传播先进文化是新闻传媒的根本任务

江泽民总书记提出的兰个代表”重要思想为新闻理论的创新赋予了鲜明的时代特征,新闻传媒传播先进文化既是对历史的继承,又是新闻事业渐进发展的结果。传播先进文化的核心内容是传播马克思列宁主义、毛泽东思想和邓小平理论。新闻传媒业木身的繁荣创新是传播先进文化的必要保证,其关键是发展,是创新。     

四种豆科牧草花药和组织培养的研究

对红豆草、苜蓿、百脉根和鹰嘴紫云英的花药、茎尖和茎段进行试管培养。以B_5,N_6,MS基本培养基附加不同成分组成12种诱导愈伤培养基和3种分化培养基,筛选不同草种和组织的最适培养基,并对形成愈伤组织及分化过程中的培养方法、条件和影响因素等进行了观察研究。     

不同支持物对地灵(Stachys floridana Schuttl.ex Benth)试管苗生根的影响

以脱脂棉、琼脂、蛭石、河沙、壤土为支持物,在1/2MS+NAA1.0mg/L+6-BA0.5mg/L培养基上对地灵试管苗生根情况进行了分析。结果表明,支持物对地灵的生根有明显影响,在珍珠岩、琼脂和脱脂棉中地灵的生根率均达到100%,各处理之间差异不显著;生根质量以珍珠岩中最优,幼根能产生大量多级分枝,形成较为强大的根系,且不同支持物试管苗的移栽结果也表明,珍珠岩在地灵试管苗生根中是琼脂的理想替代物。     

论电子媒介背景下儿童文学的阅读价值

2006年9月在中国澳门召开的国际儿童读物联盟(IBBY)第30届大会,其中的议题之一就是“儿童读物与多媒体时代”。电视、电脑等电子媒介的普及,对人们接受知识和信息的方式以及生活方式产生了深刻影响,特别是对儿童的学习方式、游戏方式以及心灵发育、思维养成都产生着不可低估的影响。本文就电子媒介正在影响儿童身心发育的现实状况进行分析,提出重新认识儿童文学之于儿童精神建构的重要性,呼吁重视儿童文学阅读对于儿童心灵成长的精神价值,有针对性地倡导家庭和学校重视儿童文学阅读。     

Effects of permanent magnetic fields on the proliferation of Phalaenopsis protocorm-like bodies using liquid medium

Magnetic fields (MFs) have been applied for the first time in orchid micropropagation. Protocorm-like bodies (PLBs) – approximately 3 mm in diameter – first derived from leaf segment culture of Phalaenopsis Gallant Beau ‘George Vazquez’, were subcultured every 2 months, and served as initial explants. The proliferation of Phalaenopsis PLBs in liquid medium in the Miracle Pack^® culture system was affected by the action of different intensities and polarities of MFs: 0.1, 0.15 and 0.2 Tesla (T) at North (N) and South (S) poles. The MF of 0.1 T – S resulted in the greatest fresh weight of regenerated PLBs. The average number of neo-PLBs formed per clusters in the PLB treated by MF: 0.1–0.2 T was decreased compared to the control exposed to natural MF (5 × 10⁻⁶ T). The proliferation of PLBs under 0.15 T – MF at both N and S poles for 2 and 7 weeks demonstrated that a longer duration of exposure to an MF of 0.15 T, regardless of the polarity, resulted in greater biomass of newly formed PLBs and smaller average number of newly formed PLBs. The S pole of MF had stronger effects on Phalaenopsis PLBs proliferation than the N pole did in all treatments.     

不同基质对杜鹃红山茶容器苗生长的影响

以杜鹃红山茶为供试材料,设置8种不同基质栽培处理,比较分析不同基质栽培对杜鹃红山茶地径、苗高、叶片数和总叶绿素含量的影响。结果表明:S5(50%椰糠+40%泥炭藓+5%珍珠岩+5%粗河砂)、S6(50%腐熟木屑+20%泥炭藓+20%椰糠+5%珍珠岩+5%粗河砂)、S7(50%椰糠+20%珍珠岩+20%蛭石+10%粗河砂)3种混合基质配比物理化学性质稳定,有机质含量较高,且能较大地增加杜鹃红山茶苗木的地径、苗高、叶片数和叶绿素含量,有效促进杜鹃红山茶对养分的吸收和生长,是较为理想的配比组合,建议在生产上推广应用。     

Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid

Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the blastocysts were transferred into the uterine horn of estrus-synchronized recipients. SCNT embryos that were cultured in mSOF or CDM developed to the blastocysts stages at similar rates (26.6% vs. 22.5%, respectively). A total of 67 preimplantational stage embryos were transferred into 34 recipients and six cloned calves were born by caesarean section, or assisted or natural delivery. Survival of transferred blastocysts to live cloned calves in the mSOF and the CDM was 18.5% (to recipients), 9.6% (to blastocysts) and 42.9% (to recipients), 20.0% (to blastocysts), respectively. DNA analysis showed that all cloned calves were genetically identical to the donor cells. These results demonstrate that SCNT embryos cultured in CDM showed higher viability as judged by survival of the calves that came to term compared to blastocysts derived from mSOF cultures.     

Temporal association of potato tuber development with susceptibility to common scab and Streptomyces scabiei‐induced responses in the potato periderm

Using hydroponics and novel non‐destructive pot culture systems which enable inoculation at specific tuber development stages, the dynamics of common scab infection patterns in potato were studied in order to provide more precise identification of tuber physiological factors associated with susceptibility. At the whole‐tuber level, infection percentages were greatest when Streptomyces scabiei inoculation occurred early; at 2 weeks after tuberization (WAT) 68% of tubers became infected, contrasting with late inoculation (8 WAT), when only 4% infection occurred. The first‐formed internodes were most susceptible to infection, whilst later‐forming and slower‐expanding internodes were less susceptible. Detailed tuber physiological examination of internode 2 showed that pathogen‐induced changes, including increased phellem (periderm) thickness, cell layers and phellem suberization (key physiological features believed critical to S. scabiei infection) were promoted through S. scabiei inoculation. Sequential harvesting showed enhanced phellem suberization (28% greater than the control) within 7 days of pathogen exposure, while phellem thickness and layer responses were also initiated early in the infection process (10–14 days after pathogen exposure) and these responses were independent of symptom expression. Differences in cultivar response were observed, with greater phellem suberization observed 10 days after tuberization (DAT) in the common‐scab‐tolerant cv. Russet Burbank than in the susceptible cv. Desiree. Likewise, Russet Burbank had thicker and more numerous cell layers in the phellem (up to eight cell layers) during early tuber growth (20–30 DAT) than Desiree (up to six cell layers).