QTL consistency for agronomic traits across three generations and potential applications in popcorn

Favorable agronomic traits are important to improve productivity of popcorn. In this study, a recombinant inbred line(RIL) population consisting of 258 lines was evaluated to identify quantitative trait loci(QTLs) for nine agronomic traits(plant height, ear height, top height(plant height subtracted ear height), top height/plant height, number of leaves above the top ear, leaf area, stalk diameter, number of tassel branches and the length of tassel) under three environments. Meta-analysis was conducted then to integrate QTLs identified across three generations(RIL, F2:3 and BC2F2) developed from the same crosses. In total, 179 QTLs and 36 meta-QTLs(m QTL) were identified. The percentage of phenotypic variation(R2) explained by any single QTL varied from 3.86 to 28.4%, and 24 QTLs with contributions over 15%. Nine common QTLs located in the same or similar chromosome regions were detected across three generations. Five meta-QTLs were identified including QTLs in three independent studies. Seven important m QTLs were composed of 11–26 QTLs for 4–7 traits, respectively. Only 11 m QTLs were commonly identified in the same or similar chromosome regions across agronomic traits, popping characteristics(popping fold, popping volume and popping rate) and grain yield components(ear weight per plant, grain weight per plant, 100-grain weight, ear length, kernel number per row, ear diameter, row number per ear and kernel ratio) by meta-QTL analysis. In conclusion, we identified a list of QTLs, some of which with much higher contributions to agronomic traits should be valuable for further study in improving both popping characteristics and grain yield components in popcorn.     

我校植物生产类本科专业实践教学课程考核评价标准体系探索

建立我校植物生产类本科专业实践教学课程考核评价标准体系,介绍其使用方法和考核重点,并举例说明和分析。     

西北太平洋公海秋刀鱼渔场浮游动物数量分布的初步研究

根据2005年7—10月在北太平洋E 150°~158°56′、N 42°34′~46°25′进行秋刀鱼资源探捕所获得的15个站点浮游动物样本资料,测得甲壳纲的桡足类、端足类、糠虾类、磷虾类,毛颚类、腔肠动物以及被囊动物等的代表种。其中桡足类占绝对优势,隶属于1目4科5属8种。浮游动物生物量4~699 mg/m3,均值168.6 mg/m3。根据浮游动物的种类、分布状况及优势种类的强弱,判断和分析黑潮暖流的强弱趋势,对确定秋刀鱼渔场的南北位置具有重要参考价值。     

国内图书馆危机管理研究文献的计量分析

运用文献计量分析方法,对CNKI收录的图书馆危机管理研究文献进行统计。对文献的发表时间、期刊分布、著者分布、主题内容等方面进行了统计和分析,由此反映出我国图书馆危机管理研究领域发表论文的状况,总结了该领域发表论文的一些特点,为以后相关内容的进一步研究提供借鉴。     

扁桃种质资源坚果数量性状遗传多样性和概率分级

[目的]研究新疆地区扁桃种质资源坚果主要数量性状遗传多样性,并对其进行概率分级,为新疆扁桃遗传改良和优质新品种选育提供参考依据.[方法]以收集90份新疆地区种质资源为研究对象,测定坚果单果重、坚果纵径、坚果横径、坚果侧径、坚果果形指数、果壳厚度、果仁单仁重、果仁纵径、果仁横径、果仁侧径、果仁果形指数、出仁率12个数量性...     

校园网络舆情视域下高职院校学生党员教育管理体系构建研究

高职院校学生党员思想培养是校园党务工作者的重要任务。随着互联网的快速发展,高职院校学生党员成为网络阵地的新生力量。新媒体下网络舆情因其自由性、隐秘性、多样性等特点,因此党务工作也应与时俱进,重视校园网络舆情,构建合理的管理体系,进而强化对学生党员的教育管理工作。     

通过GbF3’H基因单独沉默及其与GbCHI和GbDFR基因共沉默研究其在海岛棉中抗枯萎病功能

【目的】通过沉默海岛棉GbF3’H基因及共沉默GbF3’H、GbCHI和Gb DFR基因,研究其在海岛棉抗枯萎病中的作用。【方法】以海岛棉抗病材料06-146为研究对象,GhCLA1基因为阳性对照,空载体为阴性对照,构建海岛棉TRV2-Gb F3’H沉默载体,协同课题组前期构建的TRV2-CHI和TRV2-DFR载体,利用病毒诱导的基因沉默技术(Virus-induced gene silencing,VIGS)分别进行Gb F3’H基因单独沉默以及GbF3’H、GbCHI和Gb DFR这3种基因共沉默试验。通过实时荧光定量聚合酶链式反应(Quantitative real time-polymerase chain reaction,q RT-PCR)分析各处理样品中基因沉默情况;设置室内接种枯萎病菌试验测定病情指数,分析各沉默材料对枯萎病的抗性差异。【结果】q RT-PCR结果显示,海岛棉GbF3’H基因沉默后其在海岛棉根、茎和叶中的表达量比空载体对照低,Gb F3’H、GbCHI和GbDFR这3种基因共沉默后其在海岛棉根、茎和叶中的表达量均比空载体对照低。病情指数调查结果显示,野生型<空载体对照相似文献   

Development and evaluation of pyramiding lines carrying early or late heading QTLs in the indica rice cultivar ‘IR64’

The heading date is an important trait for determining regional and climatic adaptability in rice. To expand the adaptability of the indica rice cultivar ‘IR64’, we pyramided multiple early or late heading quantitative trait locus (QTLs) in the ‘IR64’ genetic background by crossing previously developed near-isogenic lines (NILs) with a single QTL for early or late heading. The effects of pyramiding QTLs were observed in three different climatic zones of the Philippines, Madagascar, and Japan. The early heading pyramiding lines (PYLs) headed 6.2 to 12.8 days earlier than ‘IR64’ while the late heading PYLs headed 18.8 to 27.1 days later than ‘IR64’. The PYLs tended to produce low grain yield compared to ‘IR64’. The low yield was not improved by combining SPIKE, which is a QTL that increases the number of spikelets per panicle. Conversely, ‘IR64-PYL(7+10)’ carrying Hd5 and Hd1 headed earlier, produced more tillers, and more panicles per m² than ‘IR64’, and mitigated the yield decrease in early heading. These results suggest that the effects of pyramided QTLs on heading date were consistent across various environments and PYLs could be used to enhance the adaptation of ‘IR64’ in other rice growing environments.     

水稻株高QTL及其与产量性状和抽穗期关系的研究进展

株高是一个与水稻品种丰产潜力密切相关的重要性状。主效半矮秆基因背景下的水稻株高变异,一般表现为受多基因控制的数量性状。最近的研究表明,已定位的株高QTL分布于水稻的所有12条染色体,其中4个QTL已克隆。克隆研究和QTL初定位结果表明,株高QTL往往存在对产量性状和（或）抽穗期的多效作用,可利用于提高水稻产量潜力。     

禽腺病毒血清4型感染鸡组织中NLRP3基因转录水平的实时荧光定量PCR分析

旨在分析禽腺病毒血清4型(FAdV-4)感染鸡组织中NLRP3基因的转录水平,本研究设计鸡NLRP3特异性引物,利用RT-PCR扩增NLRP3基因180 bp片段并克隆至pMD-18T载体,制备重组质粒pMD-18T-NL-RP3.以pMD-18T-NLRP3质粒作为标准品进行荧光定量PCR并建立标准曲线.通过反应条件...