软包装即食醉鱼制品细菌学品质安全分析

对上海市场常见品牌的软包装即食醉鱼制品感官、理化、细菌学品质状况进行定性和定量研究,并对潜在病原菌安全性进行评价。结果表明,产品水分含量、水分活度、pH和盐分含量分别为43.50%~56.97%,0.93~0.97,6.07~6.73,2.41%~6.63%,差别较大;细菌菌落总数、耐热菌落总数分别是1.8×102~4.2×105cfu/g,38~3 800 cfu/g;厌氧菌落总数和金黄色葡萄球菌均低于10 cfu/g。由细菌总数计数平板分离256株细菌,鉴定残存主要细菌菌群为芽孢杆菌(34.0%)、葡萄球菌(27.3%)、玫瑰小球菌(14.0%),并出现少量的棒状杆菌(5.5%),表明热杀菌强度不足。产品中残存有一定数量的蜡样芽孢杆菌,对其潜在危害有待进一步研究。     

青岛市崂山近岸海域水质营养现状与评价

根据2005年7、9月和2006年9、11月对青岛市崂山近岸海域的4次调查结果,分析并评价了该海域的营养状况。结果表明,表层无机氮、PO4-P和COD含量,仰口至港东、石老人、流清河湾海域略高于其他水域。底层无机氮、PO4-P均为仰口至港东水域含量较高,COD为太清宫湾水域含量较高,其余水域分布相对较均匀。从营养结构分析,7和11月该海域表、底层,N与P相比显得相对缺乏;9月表层,N与P相比也显得相对缺乏;9月底层N/P比值接近16∶1,氮、磷均不受限制。从营养状况看,除2005年9月份该海域水质处于富营养化水平外,其他调查月份水质均未达到富营养化。     

光合细菌(PSB)和翻富(FAMP)在温室无沙养鳖中的应用研究

一般的温室养鳖存在着鳖池底质和水质容易污染的问题，净化处理费工耗能；无沙养鳖新工艺可以妥善解决这一难题。本试验通过四个处理组──有沙、无沙、无沙＋ＰＳＢ、无沙＋ＦＡＭＰ──进行对比，显示出无沙养鳖法具有节能、低耗、高产、优质等特点。光合细菌（ＰＳＢ）对改善水质，提高鳖的产量有一定作用，但在较好的水质环境条件下其作用不明显。翻富（ＦＡＭＰ）为新型饲料添加剂及水质改良剂，在本次试验中增产效果亦不明显。     

Constructed shallow zones along navigation canals: vegetation establishment and change in relation to environmental characteristics

An Erratum has been published for this article in Aquatic Conservation: Marine and Freshwater Ecosystems 12(2), 2002, 251

• 1. In recent decades shallow zones have been constructed along navigation canals in the Netherlands which form a potential new habitat for aquatic macrophytes and helophytes absent from traditional canals.
• 2. The aim of this study was to examine the relationships between the aquatic plant vegetation that becomes established and the physical and chemical characteristics of water and sediment, in order to find the determinants of species composition and the changes therein. Data were collected in 1998 and 1999 from 80 plots in bank zones at varying stages of development since construction along two navigation canals.
• 3. Plots 3–5 years old were partly dominated by rooting submerged macrophytes such as Potamogeton pectinatus, Elodea nuttallii and Potamogeton pusillus; locally non‐rooting species occurred such as Ceratophyllum demersum, Lemna minor and Spirodela polyrhiza. Older plots contained Phragmites australis, locally mixed with free‐floating species. Hydrological isolation from the eutrophic canals was indicated by the presence of Chara vulgaris.
• 4. Characteristics of both water layer and sediment could explain the variation in vegetation composition. Rooting submerged macrophytes predominantly occurred in sites with a thin (<2 cm) layer of sediment with relatively low concentrations of organic matter; moreover, ammonium concentrations in the water layer and sediment pore water were relatively low. Stands of non‐rooting macrophytes and of Phragmites australis were characterized by a relatively thick sediment layer and high ammonium levels in the pore water. Light limitation in turbid water, associated with navigation and eutrophication, may also play a role.
• 5. Although submerged aquatic macrophytes persist for a relatively short time, shallow zones nevertheless function as a habitat for helophyte communities and contribute to a higher aquatic biodiversity than is associated with traditional banks along navigation canals.

Copyright © 2001 John Wiley & Sons, Ltd.     

Distribution of lipid droplets is an indicator for egg quality in brown trout, Salmo trutta fario

In the eggs of the brown trout, Salmo trutta fario, lipid droplets are clearly visible beneath the oolemma on the surface of the protein yolk. When examining the distribution of lipid vesicles in egg batches of different quality, four categories of lipid vesicle distribution could be distinguished: In the first category, the lipid droplets were evenly distributed throughout the egg. These egg batches had a high quality (percentage of eggs developing to eyed stage embryos: 96.2 ± 5.2). Some lipid droplets had the tendency to coalesce in one pole of the egg in the second category. This coalescing of lipid droplets was accompanied with a reduction in egg quality to about 50% of the first category (% of eyed stage embryos: 40.6 ± 15.2). In the last two categories, the lipid droplets were mostly coalesced in one or two poles of the egg and the egg quality was very low (% of eyed stage embryos: 3.3 ± 2.9 and zero, respectively). In conclusion, examination of lipid droplets distribution, in brown trout, can distinguish between high and low quality eggs in an easy and reliable way.     

Forage chain arrangement for sustainable livestock systems in a Mediterranean area

Mediterranean forage systems suffer from limited availability of fresh forage because of water deficits and extreme temperatures. Consequently, fresh forage is unavailable for at least 6–7 months a year, and farmers must buy feed to support livestock production. With the aim of overcoming these limitations, a 2‐year trial was conducted on three distinct sites in Sicily (at 10, 600 and 1200 m elevation) with thirty‐four varieties of forage species belonging to nine biennial/perennial and thirteen annual species. Results showed that by integrating grasses and legumes, species from environments with different climatic conditions enable the season of forage production to be extended from mid‐April to mid‐November. Quality traits of forage in different areas varied in relation to species and varieties. In general, the sown‐forage quality was better than in pastures and fallows in the same areas commonly used to feed animals. This also leads to a reduction in the use of supplementary feeds. Among the tested species, Lolium multiflorum and Medicago sativa emerged as the most promising for filling the forage‐deficit periods, and Trifolium spp. and Vicia sativa were found to be superior for increasing forage quality. The results are discussed in the context of adapting Mediterranean forage supplies for ensuring greater sustainability of livestock production in mountain, hill and plain areas. The proposed forage chain arrangement represents part of local potential adaptation to climate limitations and climate change.     

Characterization of Amaranthus cruentus L. seed proteins by 2-DE and LC/MS–MS: Identification and cloning of a novel late embryogenesis-abundant protein

Amaranth is taking great attention as an important cereal crop that could fulfill food requirements for the growing population, especially in developing countries. However, the protein composition of these seeds is not well known yet. We have used the proteomics tools to characterize amaranth seed proteome. About 400 proteins spots were resolved on two-dimensional gel electrophoresis (2-DE) and protein spots were analyzed by LC-MS/MS (liquid chromatography tandem mass spectrometry). Identified proteins were related to stress and defense responses, metabolic, respiratory, and oxide-reduction processes. One abundant spot was identified as a Late Embryogenesis Abundant (LEA) protein and the gene was cloned and characterized. The AcLEA cDNA contains a 418 bp open reading frame (ORF) encoding a polypeptide of 139 amino acids. Bioinformatics analysis showed that AcLEA belongs to LEAs Group 5. Proteomics is a powerful technique that could be used even in non-sequenced organisms such as amaranth. The obtained information reveals that amaranth seed, beyond the classical seed storage proteins, contains proteins related to protection against stress. The identification of these proteins opens the door to the application of new strategies to improve the quality of amaranth production.     

优质、抗稻瘟病香型水稻三系不育系繁源A的选育

以长丰B为母本,乐丰B为父本杂交配组选育保持系,再与乐丰A测交、回交转育,育成野败籼型香稻三系不育系繁源A,于2013年通过福建省品种审定。结果表明,繁源A不育性稳定、配合力强、异交率高、抗稻瘟病、米质优异有香味。     

平水日铸茶连续化自动化标准化生产及其产品品质分析

为了推广、建设在解放前一度几濒失传的历史名茶——平水日铸茶及其品牌,国家茶叶产业技术体系品质评价与精制拼配岗位与绍兴市柯桥区林业局进行了产品加工工艺规范化、技术与产品标准化研究。2010年至今,建设了平水日铸茶生产线2条,其生产出的产品品质达到并超过原来单机生产的产品品质,并总结出了一套平水日铸茶的标准化工艺参数。