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AIM:To study the relationship between myocardial HSP70 and PKC during myocardial ischemic preconditioning(IPC). METHODS: PKC inhibitor, polymyxin B(PMB) and PKC activator, phorbol 12-myristate 13-acetate(PMA) were applied to the models of myocardial ischemia/reperfusion in vivo and in vitro in rabbits, respectively, and the ventricular functions, PLA2 in the serum, and the expression of mycardial HSP70 mRNA were examined. RESULTS:IPC decreased PLA2 activity, improved the left ventricular function and increased the expression of myocardial HSP70 mRNA. Howerer, all these effects of IPC could be blocked by PMB. Interestingly, PMA mimicked IPC with attenuating the injuries of cardial myocytes and increasing myocardial HSP70 mRNA expression. CONCLUSION:PKC is involved in the myocardial HSP70 expression in case of ischemic preconditioning. 相似文献
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结合同源克隆和RACE技术克隆大鲵热应激同源蛋白70基因(hsc70)cDNA序列全长,通过半定量PCR方法分析其在大鲵肾脏、脑、肺、皮肤、肝脏、肾脏、脾脏、心脏、肌肉和垂体组织的表达特点。结果表明:大鲵hsc70基因cDNA全长为2 284 bp(GenBank登录号为HM998289),其中3′端和5′端非翻译区分别为87 bp(1~87)和253 bp(2 032~2 284),中间序列即编码区长为1 944 bp(88~2 031),编码647个氨基酸(AA);其核苷酸序列与其他物种相似性最高达83.5%,而编码的氨基酸序列则相对保守,与钝口螈的相似性达94%;根据其编码的AA序列构建进化树,结果大鲵hsc70首先与两栖类物种聚为一类,不同来源的hsc70基因与分类地位相似的物种分别聚类;半定量PCR结果表明,hsc70基因在大鲵的10种组织中都以较高的水平表达,但存在组织差异,在肾脏、脑、肺、皮肤、肝脏、胃、脾脏中表达量相对较高,在心脏中表达量次之,在肌肉和垂体中表达较低。 相似文献
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Shuming Zou Sifa Li Wanqi Cai Huaiyu Yang & Xiayun Jiang 《Aquaculture Research》2008,39(12):1322-1327
Interspecific allo-tetraploidy was induced by a combination of hybridization of Megalobrama amblycephala ♀× Megalobrama terminalis ♂ and thermal shock. A thermal shock at 40 °C for 2 min applied to eggs 33 or 26 min (τ0 : 1.40) after fertilization resulted in a significantly ( P <0.01) higher yield of allo-tetraploids than hybrid crosses without heat shock. The yield of allo-tetraploids in embryos from mid-gastrula to hatching stages was 15.7–24.4% in different thermal shock treatment groups. The allo-tetraploidy rate in 330-day-old juveniles was 9.3% and 6.7% in different year treatment groups. After 2 years of rearing, allo-tetraploidy males attained sexual maturity with milk-like milt at the age of 2+, while allo-tetraploidy females at the age of 2+, and even at 3+, did not show any signs of sexual maturation exhibiting obviously delayed maturity. Only a few allo-tetraploidy females (three individuals) showed maturation characters at the age of 4+ and these females were induced to spawn. 相似文献
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AIM:To establish a rat hyperlipidemia model for studying the aortic expression of heat shock protein 22 (HSP22), tumor necrosis factor alpha (TNF-α) and endothelial nitric oxide synthase (eNOS) and the effect of atorvastatin intervention. METHODS:Hyperlipidemia model was established in SD rats. Afterwards, the rats were divided into normal control group, high fat group and high fat+atorvastatin intervention group. The expression of HSP22 and TNF-α in the rat aortas was detected by immunohistochemical assay and the expression of eNOS was assessed by Western blotting. RESULTS:No detectable expression of HSP22 and TNF-α in the normal control group was observed. However, the expression of HSP22 and TNF-α was positive in the high fat group and the atorvastatin intervention group. The mean densities of HSP22 and TNF-α positive particles were significant lower in the atorvastatin intervention group as compared with high fat group (both P<0.05). The expression of eNOS protein in the high fat group and atorvastatin intervention group was significantly lower than that in normal control group (P<0.01). However, no marked difference of eNOS protein expression between high fat group and atorvastatins intervention group was observed. CONCLUSION: The expression of HSP22 and TNF-α in the rat aortas is increased in the hyperlipidemia rat model. This effect can be restored by atorvastatin treatment. The expression of eNOS in the rat aortas is decreased in the hyperlipidemia rat model, but this tendency could not be attenuated by atorvastatin. 相似文献
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《The Forestry Chronicle》2022,33(3):332-336