Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa

Monokaryotic strains of Helicobasidium mompa were used as vectors of a mycovirus between various H. mompa isolates to examine the transmissibility of one of the mycoviruses, totivirus (HmTV1–17 virus) in the hypovirulent isolate V17 of H. mompa. The isolates that acquired HmTV1–17 virus were also examined for any alteration in the virulence. Twelve dikaryotic isolates of H. mompa, belonging to 11 mycelial compatibility groups (MCGs) and being mycelially incompatible with isolate V17, were used as recipients of HmTV1–17 virus. Two monokaryotic isolates that were mycelially incompatible with isolate V17 and all of the recipients were also used as vectors of HmTV1-17 virus between isolate V17 and the recipients. When isolate V17 and recipients were directly paired on plate media, HmTV1-17 virus was transmitted from isolate V17 into 2 of the 12 recipients (i.e., 2 of the 11 MCGs). Two monokaryotic strains were paired with isolate V17, and the monokaryotic strains that acquired HmTV1-17 virus were then used as new virus donors. When the monokaryotic strains containing HmTV1-17 virus were paired with the 12 recipients, HmTV1-17 virus was transmitted into 7 of the 12 recipients from the monokaryotic strains (i.e., 7 of 11 MCGs). Based on these results, we concluded that monokaryotic strains could act as vectors to transmit HmTV1-17 virus into H. mompa isolates. When four of the H. mompa isolates that acquired HmTV1-17 virus were used to inoculate apple rootstock Malus prunifolia, the virulence of all of the isolates was attenuated from that of their parental isolates. Moreover, because the DNA fingerprints of the fungal isolates that acquired HmTV1-17 virus were the same as those of their parental isolates, the infection with HmTV1-17 virus is considered the cause of virulence attenuation of H. mompa.     

入世风险与农村现行双层经营制度的变迁

中国加入WTO之后 ,农民不仅要承担其他国家农民所承担的自然风险与市场风险 ,还要承担因现行双层经营制度带来的预期不确定性的风险 ,其根源在于现行的经营制度呈现出极大的“体制锁定”的色彩。要打破这种体制锁定 ,必须两条腿走路 :诱致性创新与强制性创新并举。既精减机构 ,明确政府的责、权、利 ,又扶植与培育农民联合体 ,增强市场创新主体分摊成本的能力与赢利能力 ,从而确保新体制的生命力与竞争力。     

GIS在生态土地分类中的作用

This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites, a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect, vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems. The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province. Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain Forest Ecosystem. Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China Responsible editor: Zhu Hong     

西藏阿里地区家畜良种繁育体系建设

在西藏阿里地区畜牧业生产考察的基础上，对家畜繁育现状及存在问题进行了分析。根据繁育体系建设原则及切实可行的技术路线，提出了西藏阿里地区繁育体系建设的目标及内容，并对繁育体系的建设进行了经济效益、生态效益及社会效益分析。针对繁育体系的建设，提出了切实有效的建议。     

构建“三位一体”防治网络预防青少年违法犯罪

青少年违法犯罪是当前的严重问题，造成这一局面有青少年自身的原因，也有社会环境的影响。预防青少年违法犯罪己成为迫在眉睫的问题，必须坚持学校、社会和家庭的配合，建立社会化防治网络，形成良好的外部环境，才能使青少年得以健康成长。     

埋地管道新型预应力抗滑桩的应用

介绍了埋地输油管道新型预应务抗滑桩的构成和抗滑原理，并对其受力状况进行了分析。以某油加库外滑坡地段埋地输油管道的实际应用为例，表明采用新型预应力锚索抗滑桩保护滑坡地段的埋地输油管道不仅是可行的，而且具有较好的经济性、实用性和可靠性。     

论中国引进判例机制的理论基础

成文法有其局限性 ,这种局限性是其本身固有而又是它自身无法克服的。判例制度正是克服了这一局限性的有效形式。     

The Application of GIS in Small Watershed Classification in Loess Plateau

ＴｈｅＡｐｐｌｉｃａｔｉｏｎｏｆＧＩｓｉｎＳｍａｌｌＷａｔｅｒｓｈｅｄＣｌａｓｓｉｆｉｃａｔｉｏｎｉｎＬｏｅｓｓＰｌａｔｅａｕＺｈｕＪｉｎｚｈａｏ，ＷｕＢｉｎ，ＢｉＨｕａｘｉｎｇ，ＺｈｏｕＣｈａｎｇｑｉｎｇＣｏｌｌｅｇｅｏｆＳｏｉｌａｎｄＷａｔｅｒＣ...     

东北虎雌性生殖系统解剖

对 2例 1 999年 1 0月意外死亡的东北虎雌性生殖系统进行了解剖。雌性东北虎的卵巢被覆卵巢囊 ,随着年龄增长卵巢表面凸凹不平 ,呈脑的沟回状。输卵管位于卵巢游离缘外侧 ,呈半圆形围绕着卵巢 ,输卵管壶腹部弯曲、峡部直 ;子宫与输卵管分界明显 ;子宫角长度约相当于子宫体长度的 2倍 ;阴道呈背腹压扁的管状     

农业产业化运行机制的现实分析

农业产业化是一项涉及多部门、多产业、多主体的系统工程，这一系统能否高效的运作.是实施农业产业化发展战略的关键。木文通过对农业产业化运行模式和运行机制的现实分析.阐述了在新形势下如何有效建立起农业产业化的运行机制。