TLC法测定虎杖中白藜芦醇的含量

本文建立了虎杖白藜芦醇含量测定的薄层扫描分析方法：以硅胶G为薄层吸附荆，氯仿：丙酮：乙酸：水(4：4：0．5：0．2)为展开剂，在紫外灯(365nm)下观察荧光斑点，在365n／n处并进行扫描测定，线性方程为：Y=(13．826S’10^-6)-0．8698(Y为白藜芦醇浓度ug／ml，S为峰面积)。并对虎杖茎、根、叶进行了含量测定。结果表明：用TIC法测定虎杖中白藜芦醇含量方法简便、快捷、准确度高、重复性好。虎杖鲜根茎中含量达0．548％。     

应用核酸斑点杂交法检测鹅细小病毒(GPV)

利用鹅细小病毒 (GPV)核酸探针 ,设计并建立了核酸斑点杂交法 ,用以检测 GPV核酸。该方法特异性与敏感性均较好 ,其敏感度可达 3pg。应用该方法对 4例病变典型的小鹅瘟患鹅组织器官进行检测 ,结果表明 ,患鹅脏器中 ,小肠、心、肝、胰、脾组织含毒量较高 ,其中小肠组织含毒量最高 ,而脑组织中含毒量较低。应用琼脂扩散试验验证了核酸斑点杂交法的检测结果。     

灰树花菌株的比较试验

通过固体斜面培养、平板培养和液体培养等方法,对4个灰树花菌株进行比较试验。结果表明,YtGf-4在菌丝长势、抗霉能力、耐高温能力、菌种间拮抗能力以及不同C/N比菌丝生长状况等方面表现出优良性状。     

8个豇豆新品系的比较试验

以之豇28-2为对照,对8个新选豇豆品系的早熟性、农艺性状、抗病性和产量进行了试验比较,试验结果表明,品系2-13-8和5-10-7熟性较早,后期翻花能力强,采收期长,荚长、荚粗和单荚质量有较大优势,田间对枯萎病、疫病、病毒病等表现出较强抗性,前期产量和总产量高,667 m2产量分别达3206.05、3294.98 kg/667 m2,与对照差异分别达显著和极显著水平,因此,2-13-8和5-10-7可作为长沙地区的优良早熟豇豆新品系进行深入研究。     

Important considerations in the analysis of generation means

Summary The weighted least squares (WLS) procedure has been widely used in the analysis of generation means in biometrical genetics. Two methods to carry out the analysis have been presented in the literature (Mather & Jinks, 1971; Rowe & Alexander, 1980). Neither method identified the factors that need to be taken into account when deciding which procedure to use. This paper examines the fitting of the additive-dominance model using the general principles of least squares analysis and presents the statistical basis of each procedure. Mather & Jink's method, which is more realistic according to genetic theory, is based on the assumption that the populations have nonhomogeneous variances. Rowe & Alexander's method is based on the assumption that the populations have homogeneous variances. In the latter case, there is actually no need to do a WLS analysis. The ordinary least square analysis using the original observations rather than the means, will produce the regression parameter estimates.     

Use of the SDS-sedimentation test and SDS-polyacrylamidegel electrophoresis for screening breeder's samples of wheat for bread-making quality

Summary Gelprotein or SDS-insoluble gel-forming glutenin was isolated from wheat flour by extraction with an aqueous 1.5% SDS solution. Remarkable intervarietal differences were observed both in amount and subunit composition of these proteins.The amount of gelprotein and the SDS-sedimentation volume both proved to be good parameters for the bread-making quality of wheat cultivars. A high correlation was observed between amount of gelprotein and SDS-sedimentation volume. The amount of gelprotein was therefore tentatively assumed to be the essential basis of the SDS-sedimentation test.The subunit composition of the gelprotein was studied by SDS-PAGE after reduction of SS bonds by mercaptoethanol. It was found that the average bread-making quality of wheat cultivars and progeny of the cross Atlas 66 x Atys which possessed subunits 3 and 10, coded for by chromosome 1D, was significantly higher than that of wheat samples possessing subunit 2 and 11, their allelic counterparts.     

Detecting and estimating segregation distortion and linkage between glufosinate tolerance and blackleg resistance in Brassica napus L.

Summary The segregation and linkage between glufosinate (transgenes ‘Rf3’ and ‘T177’) and blackleg resistance genes in canola (Brassica napus L.) were assessed using F₁ microspore-derived doubled haploid (DH) populations from four crosses including reciprocals, two involving the transgene ‘Rf3’ and the other two involving the transgene ‘T177’. To relax the assumption of no segregation distortion required for the conventional analysis of segregation and linkage, we employed Bailey's analysis that allows detecting segregation distortion at linked loci. The significant departures from the 1:1 segregation were detected in the crosses involving the transgene ‘T177’ but not in the crosses involving the transgene ‘Rf3’. The apparent deficit of the herbicide tolerant DH lines in the crosses with the transgene ‘T177’ is likely due to differential selection against the gametes carrying ‘T177’ during microspore culture. The linkage was strong between blackleg resistance and the transgene ‘Rf3’ but weak or absent between blackleg resistance and the transgene ‘T177’, suggesting that the two transgenes are probably inserted into distant regions of the genome. The observed linkage offers an opportunity to develop new canola cultivars with both glufosinate tolerance conferred by transgene ‘Rf3’ and blackleg resistance.     

Rank correlation among parametric and nonparametric measures of phenotypic stability

Summary There is an increasing number of stability parameters for genotypes grown in different environments. It is therefore useful to study the statistical relations between these parameters. One approach is the calculation of rank correlations between different stability parameters in empirical data sets. In the data analysed there are high rank correlations between ecovalence W_i, deviation mean square s² _di, the nonparametric measures S_i ⁽¹⁾, S_i ⁽²⁾, and two new measures R_i and L_i as well as between environmental variance S² _xi and regression coefficient b_i. The results suggest that S_i ⁽¹⁾, S_i ⁽²⁾, L_i, and R_i can be used as alternatives to W_i and the stability variance ² _i. This may be worthwhile, if certain statistical assumptions do not hold, particularly if significance testing is needed.     

Development a Coil Compactor used in High Temperature

材料和方法 1.1试验材料 　　荔枝果实采自武鸣县府城镇大同村陶蓬德果场，品种为新丰黑叶，果实成熟度为 9成半；龙眼果实采自武鸣县城东镇大同村蒙桂辅果场，品种为石硖、储良，果实成熟度分别为 8成半和 8成。 1.2采前处理 　　荔枝采收前即 2000年 6月 15日和 6月 28日分别