全文获取类型
收费全文 | 7365篇 |
免费 | 305篇 |
国内免费 | 1278篇 |
专业分类
林业 | 414篇 |
农学 | 682篇 |
基础科学 | 2587篇 |
1536篇 | |
综合类 | 2266篇 |
农作物 | 172篇 |
水产渔业 | 255篇 |
畜牧兽医 | 556篇 |
园艺 | 106篇 |
植物保护 | 374篇 |
出版年
2024年 | 77篇 |
2023年 | 187篇 |
2022年 | 321篇 |
2021年 | 319篇 |
2020年 | 327篇 |
2019年 | 395篇 |
2018年 | 248篇 |
2017年 | 379篇 |
2016年 | 479篇 |
2015年 | 388篇 |
2014年 | 450篇 |
2013年 | 513篇 |
2012年 | 671篇 |
2011年 | 646篇 |
2010年 | 495篇 |
2009年 | 420篇 |
2008年 | 350篇 |
2007年 | 413篇 |
2006年 | 343篇 |
2005年 | 284篇 |
2004年 | 201篇 |
2003年 | 178篇 |
2002年 | 125篇 |
2001年 | 90篇 |
2000年 | 89篇 |
1999年 | 80篇 |
1998年 | 81篇 |
1997年 | 72篇 |
1996年 | 63篇 |
1995年 | 45篇 |
1994年 | 41篇 |
1993年 | 40篇 |
1992年 | 28篇 |
1991年 | 20篇 |
1990年 | 29篇 |
1989年 | 19篇 |
1988年 | 22篇 |
1987年 | 9篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1979年 | 2篇 |
1956年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有8948条查询结果,搜索用时 234 毫秒
41.
Ibrahim Bala Salisu Ahmad Ali Shahid Amina Yaqoob Ambreen Gul Abdul Qayyum Rao 《Journal of animal physiology and animal nutrition》2021,105(2):354-363
Recent studies have demonstrated a strong relationship between the intestinal microbiota and the host health. As such, consumers are increasingly becoming more concerned about the potential effect of certain foods/feeds, particularly of transgenic origin on the gut microbiota. Although the European Food Safety Authority has recommended in their guidelines, to study the effect of transgenic food/feed on host-microbiota, yet, few studies have focused on the evaluation of such effects mainly due to culturing difficulties. Therefore, this study was intended to evaluate the potential adverse effects of transgenic diet consumption on some specific gut microflora (Lactobacillus group, Bifidobacterium genus, Escherichia coli subgroup and Enterococcus genus) of rabbits. A total of forty-eight rabbits were randomly assigned into four groups and fed a diet containing a variable proportion of transgenic cottonseeds at 0, 20, 30 and 40% inclusion level, respectively. Changes in the specific or total faecal bacterial population were monitored at five different experimental stages (i.e. 0, 45, 90, 135 and 180 days) using both the traditional plate count method (TM) and quantitative real-time PCR (qPCR). No significant differences (p > .05) were observed concerning numbers of specific bacteria or total bacteria between the control and experimental groups, though qPCR showed numerically higher values in terms of 16S rRNA gene copies as compared to the values obtained from TM. However, such numerical differences were biologically insignificant (p > .05). Similarly, no significant variations were noticed in the calculated B/E (log10 copies of Bifidobacterium per g faces/log10 copies of E. coli genome per g faeces) ratios in all the groups. All the ratios were in the range of 1.24 to 1.30 throughout the experiment, indicating a good balance of intestinal microflora and greater resistance to intestinal disorders. It is therefore concluded that feeding transgenic cottonseeds could not adversely affect the gut microflora of rabbits during a long-term study. 相似文献
42.
A-Tai Truong Sedat Sevin Seonmi Kim Mi-Sun Yoo Yun Sang Cho Byoungsu Yoon 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(3)
BackgroundThe microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen.ObjectivesThe present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees.MethodsA procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration.ResultsUR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 104 spores/bee, and the stable detection level was ≥ 2.40 × 105 spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 104 copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min.ConclusionsUR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection. 相似文献
43.
依据GenBank公布的猪圆环病毒2型Cap基因序列,在保守区域设计特异性引物和TaqMan探针,优化反应体系,建立评价猪圆环病毒2型灭活疫苗中病毒含量的实时荧光定量PCR检测方法,对方法的特异性、敏感性和重复性进行试验,并验证灭活剂用量和灭活时间对检测结果的影响。结果显示:该方法只对猪圆环病毒2型基因有特异性扩增,其他3种对照病毒基因的扩增结果均为阴性;检测灵敏度达到102.0 TCID50/mL,比普通PCR方法高100倍;方法的重复性好,对同一样品进行10次检测,变异系数为2.28%;不同灭活剂用量和灭活时间对结果的影响较小,不会因各厂家使用的灭活剂用量和灭活时间不同,影响疫苗对比实验的公平性。该研究成功建立了一种评价猪圆环病毒2型灭活疫苗中病毒含量的实时荧光定量PCR检测方法,用于猪圆环病毒2型灭活疫苗样品中病毒抗原含量的定量,其结果可以反映不同猪圆环病毒2型灭活疫苗样品中抗原含量差异,为研究猪圆环病毒2型灭活疫苗病毒抗原含量评估方法提供了新的思路。 相似文献
44.
45.
46.
基于CFD对直通式调节阀内部流场进行了二维数值模拟,并得到了内部流动参数的可视化图形。对比25%和5%两种开度下,阀门处的压力,速度以及能量损失都存在很大的差异。25%开度下的压力、流速有明显的下降趋势,而5%开度下压力和速度则迅速变化。+两种开度下产生的涡流位置不同,5%开度下能量损失比25%开度下的要大。 相似文献
47.
在电压空间矢量脉宽调制(SVPWM)控制技术原理的基础上,提出了一种简单快速算法,该算法只有普通的四则运算,计算变得非常简单,消除了常规的空间矢量控制算法中由于三角函数和无理数的近似计算而带来的计算误差及影响计算精度和速度的缺点,使结果更加准确。因而更适合应用于基于DSP 的数字化控制。理论分析和大量的MATLAB仿真结果表明,该算法具有谐波含量低、直流电压利用率高的优点。 相似文献
48.
49.
PCR扩增了金针菇(Flammulina velutipes)异戊烯基焦磷酸异构酶基因(FvIDI)的DNA全长和开放阅读框序列.该基因的ORF全长753 bp,DNA全长923 bp,含有3个内含子和4个外显子,编码250个氨基酸,金针菇异戊烯基焦磷酸异构酶相对分子质量为28450,等电点为5.05.采用实时荧光定量PCR技术研究FvIDI基因在不同发育时期和不同温度下的表达量,结果显示,FvIDI基因在采收期菌盖中表达量最高,4℃诱导处理2~4 h和37℃诱导处理2~6 h的表达量明显高于常规温度21℃处理. 相似文献
50.