灵武长枣多糖含量测定的研究

灵武长枣样品经脱脂,脱小分子糖后,用蒸馏水沸水浴浸提、醇沉、洗涤得灵武长枣多糖,含量测定采用苯酚-硫酸比色法,用精制多糖测得其多糖对葡萄糖的换算因子。建立了一种简便、准确测定灵武长枣多糖含量的方法。结果表明:该方法测定简便可行,重现性较好,平均回收率为97.11%,所测全红灵武长枣样品中多糖含量为13.30%。     

Ion-exchange microchromatography and thiobarbituric acid colorimetry for the measurement of canine glycated hemoglobins

Nonenzymatic glycation of hemoglobin is a slow, continuous, and irreversible process which takes place during the whole lifespan of the erythrocyte. When hemolytic diseases are ruled out, the levels of glycated hemoglobins reflect the time-averaged serum glucose concentration for the preceding weeks. Canine hemoglobin also binds physiologically to intraerythrocytic glucose to form a glycated fraction which provides information on the animal's long-term glycemic status. This study describes an overall evaluation of ion-exchange microchromatography and thiobarbituric acid (TBA) colorimetry for the measurement of canine glycated hemoglobins. The intra- and inter-assay coefficients of variation (CVs) found were less than 5% in normal and diabetic canine samples, and both assays proved linear over the analytical range tested, which was wide enough to include the expected clinical values. Under our laboratory's conditions, the reference range for HbA(1) was 5.82 +/- 0.62% and for HbA(1)c was 2.35 -/+ 0.47%. Sample stability was lower using the ion-exchange procedure, with increases in HbA(1) observed after 4 days in whole blood and hemolysates stored at room temperature, after 12 days in whole blood stored at 4 degrees C, and after 7 days in hemolysates stored at 4 degrees C and -20 degrees C. In the case of TBA colorimetry, whole blood was stable for at least 21 days at room temperature and at 4 degrees C, and hemolysates were stable for 18 days at room temperature, at least 21 days at 4 degrees C, and up to 3 months at -20 degrees C.     

几种制剂对羟自由基作用的应用实验

测定P－转移因子、Bio-Plus、丹参对Fenton反应产生的羟自由基的清除作用。结果表明，这些制剂可清除Fenton反应产生的羟自由基，因此，此法可用于羟自由基清除剂的筛选。     

箬竹叶总黄酮含量的测定

用N aNO2-A l(NO3)3比色法在510 nm处测定箬竹叶乙醇提取液吸光度,以芦丁为标准品,建立了芦丁标准工作曲线回归方程:c(m g.L-1)=95.43A-0.735 9,相关系数r=0.999 8.通过稳定性实验确定了显色后溶液吸光度的测定时间;通过正交实验确定了水浴提取和超声提取的最佳条件,并实验了提取次数对箬竹叶总黄酮含量的影响,测得箬竹叶总黄酮含量为1.38%;通过回收率实验,表明此法测定箬竹叶总黄酮含量准确可靠.     

洋槐花总皂苷提取工艺及抗氧化活性研究

试验以洋槐花为原材料,利用单因素及响应面优化超声波辅助醇浸提法提取总皂苷工艺条件,考察总皂苷提取物的抗氧化活性.结果 表明,液料比20 mL/g、超声频率80 W、乙醇体积分数80％、提取时间30 min、提取温度50℃时,总皂苷最大提取率为17.740％,与预测值17.742％接近;当洋槐花总皂苷浓度为5g/L时,A...     

基于盐酸-镁粉反应比色法的梭洞学中总黄酮含量的测定

[目的]对梭洞学（Polygonam capitarum D.Don）全草中的总黄酮含量进行测定。[方法]以槲皮素为对照品,采用盐酸-镁粉反应比色法测定总黄酮含量。[结果]样品中总黄酮在浓度0.007143～0.07143mg/ml范围内与吸光度呈良好的线性关系,回归方程为y=1.1760x＋0.0076（R^2=0.9998）,回收率为99.54%,RSD=0.95%（n=5）。[结论]该方法准确,简便,可用于梭洞学中总黄酮含量的测定。     

不同产地红天麻总酚含量测定

[目的]测定湖北宜昌、陕西宁强、云南昭通3个不同产地所产红天麻中的总酚含量。[方法]以没食子酸为对照品,采用Folin—Cioeaileu比色法测定不同产地所产红天麻中的总酚含量。[结果]3个不同产地红天麻的总酚含量有明显差异,湖北宜昌红天麻总酚含量最高,为0．0485％。[结论]Folin—Ciocaileu比色法为科学评价天麻质量提供一种简便快速的方法。     

黄颡鱼不同器官过氧化氢酶活性比较

[目的]比较黄颡鱼不同器官中过氧化氢酶活性。[方法]采用不连续聚丙烯酰胺凝胶电泳法和钼酸铵比色法,测定黄颡鱼6种组织（心脏、肝脏、鳃、肠、眼、肌肉）中过氧化氢酶的活性,并对各器官的酶活性进行比较。[结果]2种测定方法得到的过氧化氢酶活性具有一致性,且黄颡鱼6种组织的过氧化氢酶活性,由大到小依次为心脏、肝脏、鳃、肠、眼、肌肉。[结论]黄颡鱼的过氧化氢酶活性,存在明显组织差异性。     

气相色谱-火焰光度检测器测定菠菜中有机磷农药

建立了气相色谱-火焰光度检测器测定菠菜中有机磷农药的方法,对提取和纯化、仪器条件、微波处理与样品净化、线性方程、检测限和定量限、回收率和精密度、不确定度等进行了设计与考察,平均回收率为73%~118%,检出限为1.1~10.0μg/kg,农药分析不确定性低于16%。采用市售菠菜为样品,进行了方法可行性验证。菠菜采用微波处理50 s后加入50 m L乙腈,使用T18均质器匀浆,采用固相萃取法净化,利用气相色谱-火焰光度检测器测定。结果表明,该方法可用于检测菠菜有机磷农药残留。