Ace and ace-like genes of invasive redlegged earth mite: copy number variation,target-site mutations,and their associations with organophosphate insensitivity

Background

Invasive Australian populations of redlegged earth mite, Halotydeus destructor (Tucker), are evolving increasing organophosphate resistance. In addition to the canonical ace gene, the target gene of organophosphates, the H. destructor genome contains many radiated ace-like genes that vary in copy number and amino acid sequence. In this work, we characterise copy number and target-site mutation variation at the canonical ace and ace-like genes and test for potential associations with organophosphate insensitivity. This was achieved through comparisons of whole-genome pool-seq data from alive and dead mites following organophosphate exposure.

Results

A combination of increased copy number and target-site mutations at the canonical ace was associated with organophosphate insensitivity in H. destructor. Resistant populations were segregating for G119S, A201S, F331Y at the canonical ace. A subset of populations also had copy numbers of canonical ace > 2, which potentially helps overexpress proteins carrying these target-site mutations. Haplotypes possessing different copy numbers and target-site mutations of the canonical ace gene may be under selection across H. destructor populations. We also detected some evidence that increases in copy number of radiated ace-like genes are associated with organophosphate insensitivity, which might suggest potential roles in sequestration or breakdown of organophosphates.

Conclusion

Different combinations of target-site mutations and (or) copy number variation in the canonical ace and ace-like genes may provide non-convergent ways for H. destructor to respond to organophosphate selection. However, these changes may only play a partial role in organophosphate insensitivity, which appears to have a polygenic architecture. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

基于土地利用/覆盖的甘肃省景观生态风险时空变化特征

甘肃省景观类型丰富多样,在中国西部地区的生态安全中起关键性作用,也为当地发展提供着重要的物质基础和生态服务。由于气候变化和人类不合理活动的影响,甘肃省部分地区生态环境的安全与稳定遭到严峻挑战。本研究基于欧洲航空局全球土地利用/覆盖数据（CCI-LC）,利用动态度、转移矩阵方法分析了2005-2019年甘肃省土地利用/覆盖变化特征;通过构建景观生态风险指数并运用空间自相关分析法分析了景观生态风险的空间分布特征。结果显示：1)2005-2019年间,裸地面积减少最多,变化率1.88%,耕地次之,变化率2.31%;草地面积增加最多,变化率1.83%,建设用地次之,变化率191.08%。2）所有土地利用/覆盖类型中,建设用地的动态度最高且在整个研究时期内变化速率都保持在较高水平,综合动态度最大的时期为2010-2019年。3）土地利用/覆盖转移矩阵主要表现为耕地、林地、草地、裸地之间的转入与转出以及其他土地类型向建设用地的转入。4)2019年整体的生态风险值较2005年明显上升。其中低风险区和较低风险区面积最多,转化最频繁;中等风险区、较高风险区和高风险区面积虽然较少,但都存在不同程度的增长。...     

土地利用动态监测中多源影像融合方法的比较研究--以陕北黄土丘陵沟壑区为例

在陕北黄土丘陵沟壑区,采用单一传感器的遥感影像提取土地利用信息,存在着识别的土地利用类别少、某些类别混分现象较严重、分类结果的精度较低等问题。以TM多光谱数据和SPOT全色光谱数据的融合为例,提出了适宜于该地区的两种影像融合方法:主成分变换法和乘积运算法,并从影像的光谱质量、纹理信息和目视效果等方面对其进行了对比与评价。结果显示,主成分变换法为较理想的融合方法。以陕北无定河流域为实验样区的土地利用自动分类结果表明,该方法的应用使土地利用各类别的提取精度都有不同程度的提高;水体、水田和城镇用地等面积较小的类别分类正确率提高达到10%以上;坡耕地与林草地的混分明显减少,分类精度均提高了5%以上;分类总精度从82.0%提高到89.2%,取得了良好的分类效果。此研究对于遥感影像融合技术的评价与应用进行了有益的探索,同时为该地区的土地利用动态监测提供了关键技术。     

From Lujiazui in Shanghai to New East Area in Chongqing--An exploration on Urban Land Use Form Oriented by the Public Traffic

To develop the public traffic and setup a city form coordinated with the development of the public traffic has become a common sense in the full achievement of the city sustainable development. Due to the particularity of the exploration procedure and some difficulties in urban development, the policy on developing public traffic has not been realized in our country. Now it is on edge to solve these problems, particularly in the new area of development. In this article, the authors intend to find a practical way of establishing a ciity development mode with adapting the public traffic development by contrasting Lujiazui new area in Shanghai and the new east area in Chongqing.     

Study on the Land Use Right in Urban Housing Dismantling and Moving

With the strengthening of constitutional protection that legal private property is not to be encroached upon and the land usufructs paid regime are further clear, the regime of urban housing dismantling and moving is facing a vigorous test. This paper analyzes three problems: the justice of the withdrawal of land use right, the legality of the alteration of the land use right and the rationality of the compensation of the land use right during the urban housing dismantling and moving. Finally, this paper tries to find some effective measures to solve the chain problems.     

Exploring Harmonious Protection of Land Resources in City Construction

Urbanization in China is at an essential point in its transformation from the initial stage to accelerating stage. With quick expansion of city scale and large occupation of land for urban construction, the contradiction between land resource shortage and people's living needs becomes more and more prominent. It has great affection on the grand plan of China's sustainable development of economy and society. How to adjust the relations between the development of urban construction and protect the land resource, promote the rational use of urban land, perfect and complete the protection system of land resource for improving the efficiency of urban land use. In order to conserve land resource and find a sustainable way for development of people's living and environment construction, the above mentioned problems are the most important issue for urban construction and important subject of this paper.     

Effect of soil matric potential on tomato yield and water use under drip irrigation condition

Field experiment was carried out to investigate the effect of soil matric potential (SMP) on tomato yield, evapotranspiration (ET), water use efficiency (WUE) and irrigation water use efficiency (IWUE) under drip irrigation condition in North China Plain. The experiment included five treatments, which controlled SMP at 0.2 m depth immediately under drip emitter higher than −10 (S1), −20 (S2), −30 (S3), −40 (S4) and −50 kPa (S5), respectively, after tomato plant establishment. The results showed that different SMP affected irrigation amount and tomato ET. Irrigation amount decreased from 185 mm (S1) to 83.6 mm (S5) in 2004, and from 165 mm (S1) to 109 mm (S5) in 2005, respectively. The ET decreased from 270 mm (S1) to 202 mm (S5) in both years. However, it was found that SMP did not affect the tomato yield significantly, for the range of SMP investigated. Both WUE and IWUE increased as SMP decreased. The maximum WUE (253 and 217 kg/ha mm) and IWUE (620 and 406 kg/ha mm) were for S5 in 2 years, whereas the minimum WUE (178 and 155 kg/ha mm) and IWUE 261 and 259 kg/ha mm) were for S1 in 2004 and 2005. Based on the above results, therefore, it is recommended that if the tomatoes are well irrigated (SMP is higher than −20 kPa) during establishment, controlling SMP higher than −50 kPa at 0.2 m depth immediately under drip emitter can be used as an indicator for drip irrigation scheduling during following period of tomato growth in North China Plain.     

An evaluation of the Porac River irrigation system

An irrigation water management research programme was implemented by Hydraulics Research in collaboration with the National Irrigation Administration of the Philippines on the Porac Area of Porac Gumain Rivers Irrigation System. The objectives of the study are to monitor the response of the main canal network to changes in water availability and assess its capability to deliver crop water requirements to sub-areas jointly managed by National Irrigation Administration staff and existing Irrigator Associations. This paper presents an analysis of the response of the main system to the varying irrigation demands, and assesses the conjunctive use of groundwater with surface water and the potential of computer models to assist management with the preparation of the annual irrigation schedules.