饲粮不同水平蚕沙对绵羊生长性能、屠宰性能、器官发育和血清生化指标的影响

本试验旨在研究饲粮中添加不同水平蚕沙对绵羊生长性能、屠宰性能、器官发育和血清生化指标的影响。试验选用32只90日龄绵羊,随机分成4组,每组8只。各组饲粮中蚕沙水平分别为0(对照)、20%(T20)、30%(T30)、40%(T40)。预试期15 d,正试期60 d。试验结束后每组随机选取3只羊屠宰。结果表明:1)T20和T30组的平均日增重(ADG)高于对照组(P0.05),显著高于T40组(P0.05),日均采食量(ADFI)显著低于对照组(P0.05),料重比(F/G)低于对照组(P0.05),ADFI和F/G显著低于T40组(P0.05)。2)T20和T30组屠宰率与对照组无显著差异(P0.05),而显著大于T40组(P0.05);对照组与T20、T30组胴体重差异不显著(P0.05);T20、T30、T40组的眼肌面积和胴体脂肪含量值均显著大于对照组(P0.05)。3)T20组的心脏重量显著大于对照组(P0.05),T30和T40组的脾脏重量显著大于对照组(P0.05)。4)对照组血清球蛋白(GLO)含量显著小于T40组(P0.05)。试验组血清碱性磷酸酶(ALP)活性显著低于对照组(P0.05)。综合以上,饲粮中添加蚕沙能够增大绵羊ADG,提高屠宰率,降低料重比,蚕沙在饲粮中的适宜比例为20%~30%,最佳比例为20%。     

A framework for assessing the efficacy of antimicrobials in the control of necrotic enteritis in broiler chickens

This review presents a framework for assessing the efficacy of antimicrobials used to control necrotic enteritis (NE) caused byClostridium perfringens (CP) in the context of susceptibility testing and clinical efficacy, and their potential interactions with the intestinal microbiota of poultry. Practitioners have traditionally based their choice of antimicrobial agent on antimicrobial susceptibility testing, but there appears to be a lack of correlation with clinical efficacy for in-feed antimicrobials (particularly bacitracin and virginiamycin). Resistance patterns of CP and antimicrobials have been monitored using epidemiological cutoffs for minimal inhibitory concentration (MIC), which are not intended to guide therapy. Also, most data have been determined using CP isolates from healthy birds (i.e., potentially from commensal strains not known to be clinically relevant). It is believed that NE is caused by specific virulent CP strains (and potentially other bacteria) that proliferate and displace these commensals. The presence of resistant commensals is not necessarily detrimental (and may even be beneficial) if they inhibit the single CP strain dominance effect observed in acute NE. The choice of antimicrobial therapy in a clinical setting should thus be based on a variety of factors, including an accurate diagnosis, results of efficacy studies, prior experience at the premises in question, and interpretation of MIC data, recognizing that it is not necessarily well correlated with clinical efficacy.     

Cortisol Concentrations in Well‐Regulated Dogs with Hyperadrenocorticism Treated with Trilostane

Background

There are no clear treatment guidelines for dogs with clinically well‐regulated hyperadrenocorticism in which serum cortisol concentrations before and after an ACTH stimulation test performed 3–6 hours after trilostane administration are < 2.0 μg/dL.

Objective

To determine if serum cortisol concentrations measured before (Pre1) and after (Post1) ACTH stimulation at 3–6 hours after trilostane administration are significantly lower than cortisol concentrations measured before (Pre2) and after (Post2) ACTH stimulation 9–12 hours after trilostane administration, in a specific population of dogs with clinically well‐regulated hyperadrenocorticism and Pre1 and Post1 <2 μg/dL.

Animals

Thirteen client‐owned dogs with clinically well‐regulated hyperadrenocorticism and Pre1 and Post1 serum cortisol concentrations <2.0 μg/dL 3–6 hours after trilostane administration.

Methods

Prospective study. Dogs had a second ACTH stimulation test performed 9–12 hours after trilostane administration, on the same day of the first ACTH stimulation test. Cortisol concentrations before and after ACTH stimulation were compared using a paired t‐test.

Results

Cortisol concentrations before (1.4 ± 0.3 μg/dL) and after the first stimulation (1.5 ± 0.3 μg/dL, mean ± SD) were significantly lower than cortisol concentration before the second stimulation (3.3 ± 1.6 μg/dL, P = .0012 each). Cortisol concentration before the first stimulation was also significantly lower than cortisol concentration after the second stimulation (5.3 ± 2.4 μg/dL, P = .0001).

Conclusions and clinical importance

In dogs with clinically well‐regulated, trilostane‐treated, hyperadrenocorticism, and cortisol concentrations <2 μg/dL before and after the first stimulation, a second ACTH stimulation test performed 9–12 hours after treatment can result in higher cortisol concentrations that could support continued trilostane treatment.     

Detecting endotoxin activity in bovine serum using an automated testing system

The aim of the present study was to compare the ability of the commercially available portable test system (PTS^TM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS^TM was not significantly different from that of the traditional LAL-based assays. The results using PTS^TM correlated with those using KT (r²=0.963, P<0.001) or KC assays (r²=0.982, P<0.001). Based on these findings, the PTS^TM could be applied as a simplified system to assess endotoxin activity in bovine serum.     

Phenotypic and genotypic characterization of canine pyoderma isolates of Staphylococcus pseudintermedius for biofilm formation

Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods, the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius.     

Study on Feed Value of Cassava Residue for Growing-Fishing Pigs

To determine the apparent digestibility of cassava residue (CR) nutrient and the effects of CR on growth performance when replaced the different levels corn in diet,two experiments were designed.In the first experiment,8 Duroc×Landrace×Yorkshire crossbred barrow pigs with the similar weight (22.46 kg±1.08 kg) were chosen for determining the apparent digestibility of CR.The pre-test period lasted for 10 days,and the trial period lasted for 5 days.In the second experiment,160 Duroc×Landrace×Yorkshire crossbred pigs with the similar weight(21.09 kg±0.72 kg) were randomly divided into five groups with four replicates per group and eight pigs per replicate.The control group was fed with basal diet while the experimental groups were fed diets containing 5%,10%,15% and 20% levels of substitution of corn by CR,respectively.The pre-test period lasted for 5 days,and the trial period lasted for 30 days.The results showed that the content of CP and EE in CR were 2.73% and 0.68%,but CF and total energy were 15.34% and 13.55 MJ/kg.The content of Lys and Thr were 0.11% and 0.08%,respectively.Calcium and phosphorus apparent digestibility were low,just only 40.24% and 34.76%,respectively,while apparent total energy digestibility was 65.62%.The apparent digestibility of Arg,Glu,Tyr,Met,Leu and Val were higher than 80%.The average daily gain was reduced with increasing dose of CR,which the group of 15% and 20% addition were significantly lower than that of control group (P< 0.05).However,the average daily gain of 5% and 10% groups were no significant difference with the control group(P >0.05).The feed cost of 5% and 10% addition groups were decreased by 0.01 and 0.05 yuan.In conclusion,under this experimental condition,the appropriate addition of CR for growing-fishing pig (20 to 50 kg ) was 5% to 10% in the diet.     

木毒蛾核多角体病毒（LxNPV）杀虫剂：Ⅱ.制剂的研制

通过室人工饲毒养虫并结合林间防治木毒蛾，回收病死虫尸，获取毒源，添加各种辅助剂，配制成９０１－１可湿性粉剂和９０１－２乳剂，进行林间大面积防治木毒蛾试验，１０天防治效果达８０％以上。实验表明，丙酮－乳糖共沉法回木毒蛾核多角体病毒，回收率可达８０．１７％。     

播种时期对蒙古栎不同类型苗木出苗和生长的影响

以蒙古栎种子为材料,研究播种时期对蒙古栎不同类型苗木出苗和生长的影响,结果表明:蒙古栎在秋季不同时间播种的出苗率均在60%以上,但以早期(9月下旬)播种的出苗率最高(大于80%),早期播种的苗木在苗高、地径、侧根数、根体积和根生物量等指标上都明显要高于其它播种时期,蒙古栎在秋播裸根育苗时应适时早播;秋季播种的容器苗在所有观测的形态指标上均好于春季播种的容器苗,秋季容器育苗质量更高。