Effects of ERK1/2 on pressure overload-induced cardiomyopathy and heart failure in mice

AIM: To explore the changes in extracellular regulated protein kinase (ERK1/2) in the hypertrophic myocardium induced by pressure overload at the different time courses and to determine the molecular mechanism in the myocardium from hypertrophy to heart failure. METHODS: C57/BL mice, aged 12 week old, were subjected to sham-operation (SH) or transversing aortic constriction (TAC) to establish left ventricular hypertrophy. Echocardiographic assessments, hemodynamic determination, organ weight measurement, morphological and histological examination were performed at 1, 4, 8, 12 and 16 weeks after surgery. Meanwhile mRNA levels of atrial natriuretic peptide (ANP), α-myosin heavy chain (α-MHC), bcl-2 and bax were measured by RT-PCR, and ERK1/2 levels were detected by Western blotting. The animals in SH group were performed the same tests then sacrificed at 16 weeks. RESULTS: (1) Compared to SH group, LVESd, LVEDd, Awsth, Awdth, Pwsth and Pwdth progressively increased after TAC. Meanwhile, ejection fraction (EF%) significantly decreased at 16th week (P<0.05). LVSP, dp/dtmax and dp/dtmin in TAC group were progressively increased after 4 weeks. From 8-12 weeks these parameters maintained stable and then sharply decreased at 16th week (all P<0.05). However, LVEDP was statistically increased at 8th week. These echocardiographic and hemodynamic changes indicated a development of LVH and eventually progressing towards to heart failure. (2) Histologically, cardiac collagen measured by percentage of Sirius red positive stained area and apoptosis index showed progressive increases from 4 to 16 weeks. (3) Compared to SH group, mRNA levels of ANP was time-dependently increased while α-MHC and Bcl-2 were time-dependently decreased. The ratio of Bcl-2 /Bax was decreased. Phosphorylation of ERK1/2 was increased at 4th week, then decreased with age of TAC (all P<0.05). CONCLUSION: Pressure-overload induced by TAC results in a development of LVH from early concentric hypertrophy to late eccentric hypertrophy, and eventually toward cardiac dysfunction or heart failure. Those changes are associated with increase in cell size and cardiac fibrosis. ERK1/2 signaling pathway may involve in the regulation of myocardial cell apoptosis in hypertrophic and failure heart.     

闽浙木拱廊桥遗产旅游产品开发研究

近年来阂浙木拱廊桥研究逐渐被人们所重视。阖浙22处木拱廊桥共同“中遗”使木拱廊桥的开发和保护迎来一个新的契机。闽浙木拱廊桥可以基于遗产旅游视角开发成为民俗文化旅游产品、生态休闲旅游产品、乡村旅游产品、红色旅游产品、节庆旅游产品等5类旅游产品。在廊桥遗产旅游产品开发过程中应注意廊桥遗产真实性和完整性的保护,注重旅游景观和产品体验性开发,形成良好的保护与开发机制,协调好利益相关者之间的关系,加强遗产文化教育和宣传,促进区域联动发展。     

刚架拱桥斜撑破坏及加固后可靠性分析

根据刚架拱桥结构的受力特性和构件本身存在的缺陷,利用斜撑的ANSYS实体模型分析刚架拱桥斜撑的破坏机理,以弹模的降低来模拟斜撑湿接缝处混凝土强度的衰变.研究表明湿接缝处混凝土强度不足是斜撑破坏的主要原因,并对斜撑加固后的可靠性进行分析,预测了斜撑的剩余使用寿命.     

钢拱架塑料大棚棚体结构的应力测试

本文对设计研制的LPB—10型钢拱架塑料大棚棚体结构的模拟试验结果进行了分析研究,并与设计计算结果进行了对比分析。认为原设计方案是可行的,计算数据可靠,可在华北、华中、西北及东北大部地区推广使用。     

万家沟水库混凝土双曲拱坝施工控制网的布置

本文根据万家沟水库施工控制网的实际设计、施测情况，就其设计特点、依据以及满足施工控制范围和精度要求所采取的措施进行了阐述，论证了该控制网设计的可行性和在施测中所采取的措施的可靠性。     

contact stress and steady thermal analysis of herringbone gears in a gas turbine

To obtain the distribution characteristic of contact stress and the bulk temperature on the surface of herringbone gears, parameter modeling of gears was carried out by parametric design language. A numerical simulation was given to calculate the contact stress field and the steady thermal field by combining heat transfer theory, tribology theory, and mesh theory. The distribution of contact stress, the bulk temperature and heat flux also were analyzed. The analysis results show that the distribution of the load along the contact line possesses regularity. The heat flux along the tooth depth direction decreases first and then increases. The effect of thermal conduction of the tooth is stronger than that of convective heat transfer. The conclusion indicates that the finite element emulation method can be used to estimate the transmission performance of herringbone gears.     

农业干旱风险研究进展与发展趋势

随着农业旱灾对农业系统造成的损失日益加剧,增大了对区域资源的压力,造成一系列环境问题,农业干旱风险分析逐渐成为近年一个新的研究领域;这一研究不仅是农业旱灾风险管理的基础和前提,也是农业干旱风险区划和灾前损失预评估的理论基础。目前,针对农业干旱风险的研究大多是通过模拟特定干旱指标与作物生物量之间的关系,对作物因旱损失进行微观风险评价,因此在阅读大量文献的基础上,分别对农业干旱、农业干旱风险的定义,以及对基于降水指数的农业干旱风险的研究进展进行了综述。     

型钢拱架日光温室结构稳定性能及参数分析

型钢拱架日光温室结构的主要受力构件长细比大,暴雪等极端灾害天气下易引发结构失稳灾变。针对此问题,该研究利用弹塑性力学理论和非线性有限单元法,建立型钢拱架日光温室结构精细化有限元模型,开展雪荷载下日光温室稳定性能分析;通过对型钢截面类型（平椭圆形截面、箱形截面和几字形截面）、温室跨度（8、10和12 m）、雪荷载分布形态（分布厚度不均匀和分布区域不对称）等参数下进行日光温室失稳全过程分析,分别确定日光温室稳定承载力,揭示雪荷载分布对日光温室稳定承载力的量化影响;结合日光温室的荷载系数-位移全过程曲线和不同加载时刻点的变形图、应力图、轴力图与弯矩图,从直观现象和内在本质两个层面深入探明日光温室的静力失稳机理。分析结果表明：在保证不发生平面外整体失稳的前提下,当型钢截面面积和翼缘宽度相同时,相较于箱形截面型钢、几字形截面型钢,采用平椭圆形截面型钢拱架的日光温室稳定承载力分别提高了19.2%和44.2%;跨度对日光温室稳定承载力的影响较大,与8 m跨度相比,10、12 m跨度的日光温室的荷载系数分别下降了27.1%和57.9%;相较于均匀分布雪荷载,在非均匀分布雪荷载下日光温室的稳定承载力最大下降63.8%;相较于不设置拉杆和撑杆的情况,单独设置拉杆的日光温室稳定承载力最大可提高9.0%,单独设置撑杆的日光温室稳定承载力最大可提高66.8%。该研究得出的结果和给出的建议可为型钢拱架日光温室结构抗雪设计、稳定性研究和防灾分析提供技术指导和理论参考。     

浅谈地铁暗挖隧道软弱土层施工方法

介绍了哈尔滨哈西联络线——哈西大街站至医大二院站暗挖区间穿越软弱地层时所采用的几种施工方法和措施。     

悬链线单肋无铰拱横向稳定性研究

在总结前人研究成果的基础上，重点探讨了悬链线单肋无铰拱在竖向荷载作用下，横向稳定影响的实用计算方法。在分析拱轴系数、矢跨比、拱肋抗扭刚度与横向抗弯刚度比对单肋拱横向稳定的影响趋势和大小的基础上，通过数值积分，给出上述各因素影响单肋拱横向稳定临界荷载的曲线，为拱桥设计时拱肋的结构参数的选取提供一定的依据。