稻谷添加专用酶替代部分玉米时肉鸭生长及物质代谢的影响

本试验采用对照试验设计,以常规的玉米一豆粕为主（不含稻谷）的日粮为对照组,在对照日粮基础上用20％的稻谷替代20％的玉米并添加稻谷专用酶制剂的日粮为试验组,以肉鸭为试验对象进行为期50天的比较研究。结果表明．相对与对照组,试验组的肉鸭的生长性能、养分利用率差异不显著（P〉0．05,可明显提高经济效益;试验组的日粮CF（粗纤维）表观消化率提高了12．6％（P〈0．05）。DM（干物质）、CP（粗蛋白）、EE（粗脂肪）的表观消化率分别降低了2．62％、1．88％、2．59％（P〉0．05）,说明用稻谷代替鸭日粮中的部分玉米是可行的。     

三菌混合固态发酵苹果渣产复合酶的研究

利用实验室保藏的多株菌种,对苹果渣进行三元混菌固态发酵产复合酶。以纤维素酶、木聚糖酶、果胶酶活性大小为测定指标,对筛选出单菌活性较高的菌株,按照1:1:1进行3种菌混合发酵,确定混菌后效果较好的3种菌。考虑到试验因素之间的交互作用,以3种菌的接种比例及接种时间间隔作为试验因素,通过正交表交互作用设计试验及分析,确定3种菌混合活性最佳时间间隔为菌种#1、#2、#3同时接种;接种#1体积1ml、接种#2体积2ml、接种#3体积1ml。混菌后酶活性比3种单菌的酶活分别提高了10.9%、2%、20%,达到了优化的目的。     

东亚飞蝗FK506结合蛋白基因克隆及其免疫功能分析

为明确东亚飞蝗Locusta migratoria manilensis免疫相关FK506结合蛋白(FK506 binding protein,FKBP)的功能,了解FKBP对金龟子绿僵菌Metarhizium anisopliae侵染东亚飞蝗的影响,分离克隆获得FKBP46基因及其纯化蛋白,采用荧光定量PCR方法测定...     

单宁酶性质及其分析方法研究进展

介绍了产单宁酶的菌种及不同来源的单宁酶理化性质差异,阐述了单宁酶的酶学性质、分子生物学特征、催化反应机理和单宁酶的分析方法等的研究进展。     

中药复方发酵粉对断奶仔猪生长性能、养分表观消化率和消化酶活性的影响

试验旨在研究日粮中添加中药复方发酵粉对断奶仔猪生长性能、养分表观消化率和消化酶活性的影响。选择32头体重为（14.96±0.96）kg的健康42日龄"杜×长×大"三元断奶仔猪,随机分成4个处理,每个处理8个重复,每个重复1头猪。对照组饲喂基础日粮,其余3组分别在基础日粮的基础上添加金霉素75 mg/kg、中药复方发酵粉600 mg/kg及金霉素75 mg/kg+中药复方发酵粉600 mg/kg。试验期为42 d。结果表明,日粮中添加中药复方发酵粉显著提高了仔猪的干物质、有机物和碳水化合物的表观消化率及表观消化能（P<0.05）,提高了必需氨基酸和非必需氨基酸（甘氨酸、天冬氨酸、丙氨酸、胱氨酸、酪氨酸）的表观消化率及脂肪酶活性（十二指肠、空肠、回肠）和淀粉酶活性（回肠）（P<0.05）。金霉素和中药复方发酵粉对仔猪日增重、粗脂肪和粗蛋白质的表观消化率及表观消化能和亮氨酸、苯丙氨酸、丝氨酸的表观消化率有显著的交互效应（P<0.05）;与对照组相比,金霉素组和中药复方发酵粉组显著提高了日增重、粗脂肪和粗蛋白质的表观消化率及苯丙氨酸和丝氨酸的表观消化率（P<0.05）,但两组间差异不显著（P>0.05）。由此可见,日粮中添加600 mg/kg中药复方发酵粉能提高断奶仔猪养分表观消化率、氨基酸表观消化率和肠道消化酶活性。     

中草药饲料添加剂对河西肉牛血液生化指标影响的研究

为了提升河西肉牛产品特别是血液制品的质量及产业效益,利用饲料中添加中草药添加剂的方法对河西肉牛架子牛进行了育肥、屠宰试验。采取低剂量中草药组、高剂量中草药组和对照组肉牛血样,用专门仪器和方法测定了血液中5种血清矿物质离子、7种血清代谢产物及5种血清酶等生化指标的含量。结果表明:高剂量中草药组肉牛血清中的Na、Mg离子及乳酸脱氢酶含量均极显著高于低剂量组和对照组(P<0.01),Cl离子含量极显著高于对照组(P<0.01),其葡萄糖、肌酐、甘油三酯含量均显著高于对照组(P<0.05),谷丙转氨酶显著低于其他两组肉牛(P<0.05),而谷草转氨酶(AST)却显著高于其他两组肉牛(P<0.05);低剂量组和高剂量组肉牛血清中的高密度脂蛋白极显著高于对照组(P<0.01),碱性磷酸酶极显著低于对照组肉牛(P<0.01),而谷丙/谷酸则显著高于对照组肉牛(P<0.05)。结论:试验配制的中草药饲料添加剂对肉牛血液生化指标有良好的影响,对高剂量组肉牛的影响更为明显;饲喂中草药饲料添加剂的肉牛生长发育、营养状况均显著好于对照组,但高剂量组更优;利用高剂量中草药饲料添加剂饲喂的肉牛,其血液在制造保健用品方面有广阔开发前景。     

Studies on Normal Haematological and Serum Biochemical Values of the ‘Hijin’ Racing Camels (Camelus dromedarius) in Kuwait

Haematological estimations and serum biochemical analyses were made on 100 samples collected from clinically healthy Hijin racing camels (Camelus dromedarius) in Kuwait. The red blood cell counts, packed cell volume, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and total white blood cell counts were estimated. In the serum biochemical analyses, sodium, potassium, iron, calcium, phosphorus, magnesium, total bilirubin, blood urea nitrogen, creatinine, glucose, total protein and cholesterol concentrations were measured, as were the alanine aminotransferase, aspartate aminotransferase, -glutamyltransferase, creatine kinase and alkaline phosphatase activities. The results are discussed in relation to other findings reported in camels.     

非淀粉多糖酶制剂在动物营养中的研究进展

谷物中非淀粉多糖导致日粮养分消化率和饲养效果降低,限制了谷物在饲料中的应用。本文详细论述了非淀粉多糖酶制剂消除非淀粉多糖抗营养作用的机理,对养分消化率和动物生产性能的影响以及对作用底物定量关系的研究,存在的问题及今后的研究方向。非淀粉多糖酶制剂在提高谷类籽实饲料的利用效率,开发利用非常规饲料资源方面有着巨大的应用前景。     

Cellulase Supplementation Does Not Improve the Digestibility of a High-Forage Diet in Horses

Six mature Arabian geldings were used in a two-period crossover study to investigate the effects of cellulase supplementation on fiber digestion. Horses were randomly assigned to either a control (CO; n = 3) or a cellulase (CE; n = 3) treatment for the first period and then treatments were switched for period 2. Each period consisted of a 10-day diet adaptation followed by a 3-day total fecal collection. The enzyme mixture contained 40,000 cellulase units/g and was fed at a rate of 3 g/day split evenly between two feedings. During the diet adaptation period, horses had ad libitum access to timothy hay and were also fed 165 g whole oats as a carrier for the supplement. When eating the CO treatment, horses consumed 16% more hay than when on the CE treatment (P = .004). Fecal output also tended to be greater when horses consumed the CO treatment as compared with CE treatment (P = .07). No differences were found between treatments for fecal percent dry matter (DM%), fecal neutral detergent fiber (NDF), fecal acid detergent fiber (ADF), fecal nitrogen (N), or fecal gross energy (GE). There was a trend for horses consuming the CO treatment to digest more NDF than when consuming the CE treatment (34.6% ± 1.5 vs 31% ± 1.5; P = .07). Horses also digested a greater %ADF, %N, and Mcal of energy when consuming the CO treatment than when consuming the CE treatment (P < .05). Cellulase addition to a hay-based horse diet decreased digestion of fiber components.