驱油复合菌系的构建及驱油能力的研究

为构建驱油复合菌系并对提高原油采收率的能力进行评价,在50℃条件下通过长期限制性培养,以乳化和降解石油的能力为指标,获得一组高效稳定的驱油复合菌系。复合菌系发酵液对原油具有降粘和降低表面张力的作用。室内岩心驱油试验表明,水驱后复合菌系驱可提高石油采收率14．47％。2007年9月20日在大庆杏6—3-丙605油井进行了吞吐试验,截止2008年7月31日,采出液平均含水率由93．15％降至90．69％,日产液量提高69．86％,累计增产原油203．95t。表明复合菌系适应地层油藏条件,具有增采作用。     

Method of Constructing Core Collection for Malus sieversii in Xinjiang, China Using Molecular Markers

The method for constructing core collection ofMalus sieversii based on molecular marker data was proposed. According to 128 SSR allele of 109 M. sieversii, an allele preferred sampling strategy was used to construct M. sieversii core collection, using the UPGMA （unweighted pair-group average method） cluster method according to Nei ＆ Li, SM, and Jaccard genetic distances, by stepwise clustering, and compared with the random sampling strategy. The number of lost allele and t-test of Nei＇s gene diversity and Shannon＇s information index were used to evaluate the representative core collections. The results showed that compared with the random sampling strategy, allele preferred sampling strategy could construct more representative core collections. SM, difference for construction of M. sieversii core collection. Jaccard, and Nei ＆ Li genetic distances had no significant SRAP （sequence-related amplified polymorphism） data and morphological data showed that allele preferred sampling strategy was a good sampling strategy for constructing core collection of M. sieversii. Allele preferred sampling strategy combined with SM, Jaccard, and Nei ＆ Li genetic distances using stepwise clustering was the suitable method for constructing M. sieversii core collection.     

葡萄核心种质的构建

【目的】在已建立的葡萄初级核心种质的基础上,利用SSR分子标记技术构建葡萄核心种质。【方法】利用M策略（Core Finder和Power Core）、遗传距离法（least distance stepwise sampling,LDSS和genetic distance optimization,GDOPT）和Core Hunter法构建核心种质, 并对He、Ho和 I值等遗传多样性指数和方差差异百分率（VD%）、均值差异百分率（MD%）、极差符合率（CR%）和变异系数变化率（VR%）等表型指标进行统计检验,同时采用SSR和SRAP分子标记的主坐标分析对其进行确认。【结果】M策略构建的核心种质能保留初级核心种质全部的等位基因,遗传距离法抽取的核心种质对原始种质具有较好的代表性。为使所构建的核心种质具有最大的遗传距离和最大的遗传多样性,对M策略、遗传距离法和Core Hunter法构建的核心种质进行了合并,48份葡萄核心种质以最少的种质材料保留了初级核心种质96.21%的等位基因,以5.53%的取样比例代表了原始整体种质92.90%的遗传多样性。【结论】经分子和表型检验,所构建的核心种质具有较好的代表性和遗传多样性。同时本研究所采用的方法对其它作物核心种质的构建具有重要的参考价值。     

弘扬福建精神，践行社会主义核心价值观

“爱国爱乡、海纳百川、乐善好施、敢拼会赢”的福建精神是社会主义核心价值观在福建的时代表达,是实现更加优美更加和谐更加幸福福建的强大精神动力。必须通过深挖红色资源内涵,弘扬海洋文化传统,倡导公益仁爱理念,传承闽商文化精髓等举措弘扬福建精神,践行社会主义核心价值观。     

USE OF ULTRASOUND-GUIDED FINE-NEEDLE ASPIRATION BIOPSY AND AUTOMATED CORE BIOPSY FOR THE DIAGNOSIS OF GASTROINTESTINAL DISEASES IN SMALL ANIMALS

The purpose of this study was to evaluate the usefulness of ultrasound-guided fine-needle aspiration biopsy and core biopsy in the diagnosis of infiltrative gastrointestinal diseases. Six dogs and seven cats with clinical signs of gastrointestinal disease underwent ultrasonography and intestinal lesions were identified. One or more ultrasound-guided fine-needle aspiration biopsy and/or core biopsy procedures were performed in each patient. Each patient also underwent one of the following additional procedures for comparison of results: 1) surgery (n=4), 2) endoscopy (n=2), 3) post mortem exam (n=3), and, 4) for lymphoma diagnosed with ultrasound-guided procedures, response to chemotherapy (n=4). Correct diagnoses were obtained in nine of the 13 patients, incorrect diagnoses in two of the 13 patients, and inconclusive diagnoses in two of the thirteen patients. Of the 16 total ultrasound-guided procedures performed, ten were confirmed as correct, three as incorrect, and three were non-diagnostic. Intestinal lesions with bowel wall thickness greater than 2.0 cm had a higher percentage of correct diagnoses than lesions of lesser wall thickness. Gastric lesions had a higher percentage of correct diagnoses than small and large intestinal lesions. Malignant lesions had a higher percentage of correct diagnoses than benign lesions. There were no complications. Ultrasound-guided gastrointestinal fine-needle aspiration biopsy and core biopsy appears to be a safe, accurate, and rapid procedure for use in the diagnosis of infiltrative gastrointestinal disease.     

基于小型秸秆圆捆机的秸秆摩擦特性研究

针对水稻与甘蔗秸秆利用小型秸秆圆捆机收集打捆效率低的现状,研究了圆捆机卷捆室内打捆的情况,为圆捆机的结构改进提供必要的理论和技术依据。秸秆能否在卷捆室顺利形成草芯主要涉及辊子与秸秆的摩擦因数的问题。为此,利用自制的滑动摩擦因数测试装置,对秸秆进行摩擦特性试验研究,采用三因素三水平的正交旋转组合试验设计方案,探究秸秆含水率、取样部位以及滑板材料3个变量因素对摩擦因数的影响规律。结果表明:含水率对稻秆影响最大,滑板材料对甘蔗叶影响最大,3种滑板材料都是与橡胶的摩擦因数最大。由此得出:小型圆捆机卷捆室内下半部分辊子可以考虑选用橡胶沿着钢辊锯齿形凸起表面贴紧的方式,这种胶辊对快速形成草芯更有利。     

基于介电特征的苹果霉心病检测方法

针对苹果霉心病无法有效根据外表进行识别,且传统检测方法具有设备复杂、成本高昂等问题,本研究通过采集苹果介电参数构建苹果霉心病检测模型,从而实现简单快速的苹果霉心病无损检测.基于LCR测量仪采集220个苹果的108项介电指标(9个频率下的12项介电指标)作为原始参数,使用数据标准化、主成分分析算法等对数据进行预处理,并利...     

融合多光谱成像与深度学习的作物植株叶绿素检测系统研究

为了满足田间作物长势快速检测与指导变量管理的需求,以玉米为例设计了基于多光谱成像的田间作物植株叶绿素检测系统,包括可见光(RGB)和近红外(Near-infrared, NIR)图像采集模块、主控处理器模块、模型加速模块、显示及电源模块,用于实现玉米植株智能识别与叶绿素指标一体化检测。首先,采集玉米苗期和拔节期冠层图像数据集,比较了植株冠层实例分割与株心目标检测两种深度学习模型,构建了基于MobileDet+SSDLite(Single shot multibox detector lite)轻量化网络的玉米植株定位检测模型,实现玉米植株识别。其次,提取被识别的植株株心RGB-NIR图像,开展RGB和NIR图像匹配与分割,提取R、G、B和NIR灰度值计算植被指数,使用SPXY算法(Sample set portioning based on joint X-Y distances)和连续投影算法(Successive projections algorithm, SPA)分别对数据集进行样本划分及特征变量筛选,选择高斯过程回归(Gaussian process regression, ...     

知识管理与企业核心竞争力的培育

本文阐述了知识管理和企业核心竞争力的内涵,论证了知识管理的重要性及知识管理能够创造企业的竞争优势,提高企业的核心竞争力。最后给出了知识管理所需要的条件,以作为企业进行知识管理调整和改革的参考。     

Genotypic diversity and migration of clonal lineages of Botrytis cinerea from chickpea fields of Bangladesh inferred by microsatellite markers

An analysis of allelic diversity at nine microsatellite loci provided an insight into the population structure of Botrytis cinerea from four fields (sampled in 2003 and 2004) that represented important regional locations for chickpea production in Bangladesh. Although three populations were limited by sample size after clone‐correction, a total of 51 alleles were amplified among 146 B. cinerea isolates from Bangladesh, which revealed a high amount of within‐population and overall genetic diversity (H_S = 0·48 and H_T = 0·54, respectively). The percentage of maximal genotypic diversity (G) ranged between populations (G = 23–40), with a total of 69 haplotypes detected (G = 25). Bayesian cluster analysis depicted two major clusters distributed among the four Bangladesh populations, indicating population admixture from two origins that have spread throughout these regions. Genotype flow between regions was detected and indicated the spread of clonal lineages, consistent with relatively low differentiation among the four populations (mean G_ST = 0·1, P < 0·05). These results highlighted the potential threat of host resistance breakdown as a result of considerable genetic diversity, genotype flow and the evolutionary potential of B. cinerea.