多径衰落信道下OFDM系统的符号同步方法研究

由于在正交频分复用（OFDM）的系统中存在符号时间同步误差，为了消除这种误差，提出了工作在多径衰落信道的符号时间频移（STO）估计方法，即基于CP的符号时间偏移估计和基于训练符号的符号时间偏移估计，通过仿真对基于循环序列（CP）估计技术的最大似然法（ML）和基于训练符号的最小均方差异法（Classen）两类性能比较分析，在无载波频移条件下，ML算法精确度更高，当存在载波频移时，Classen算法精确度更高。     

油菜种子低亚油酸和亚麻酸含量异交稳定技术的建立

为降低油菜种子中的亚油酸和亚麻酸含量并使之在异交条件下保持基本稳定,以高油品种CY2作为受体亲本,采用RNA干涉技术沉默油菜内源Bn FAD2基因的表达,抑制油酸脱饱和反应。育成的3个独立转基因株系的亚油酸和亚麻酸含量下降到6%以下,其中亚油酸含量较受体亲本降低78.2%~86.5%,亚麻酸含量降低53.4%~65.8%。将3个转基因株系与2个亚油酸和亚麻酸含量较高的常规品种正反交,F1种子中这2种脂肪酸的含量依然保持在与转基因亲本相仿的低水平。由此可知,通过RNA干涉技术沉默Bn FAD2基因的表达可赋予油菜低亚油酸和亚麻酸含量的异交稳定特性。这一特性的获得对于今后培育异交稳定的高油酸油菜新品种具有重要意义。     

Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells

AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G₀/G₁ phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells.     

二化螟dsRNA降解酶基因的克隆与表达分析

为解析二化螟Chilo suppressalis体内参与降解双链RNA(double-stranded RNA,dsRNA)的关键核酸酶的功能,克隆二化螟不同的非专一性核酸酶（non-specific nuclease,NUC）基因,并对这些基因进行生物信息学分析和组织定量表达分析,同时对dsRNA降解酶（dsRNA degrading nuclease,dsRNase）活力的组织分布进行研究。结果显示,共克隆获得5个NUC基因,其中有4个编码dsRNase亚家族基因（CsdsRNase1～CsdsRNase4）和1个编码Endonuclease G亚家族基因（CsEndoG）。5个NUC基因的开放阅读框核苷酸序列长度范围为828～1 338 bp,编码275～445个氨基酸残基,其分子量大小为31.68～49.57 kD,预测等电点为5.48～9.42。CsdsRNase1和CsdsRNase2含有信号肽序列,两者相似度极高,且与家蚕Bombyx mori和斜纹夜蛾Spodoptera litura中具有dsRNA降解酶活力的dsRNase同源聚类;CsdsRNase1和CsdsRN...     

草栖钝绥螨对二斑叶螨的捕食作用

为明确草栖钝绥螨Amblyseius herbicolus对二斑叶螨Tetranychus urticae的控制潜能,在温度分别为19、22、25、28和31℃、相对湿度均为（85±5）%、光周期均为16 L∶8 D条件下测定草栖钝绥螨对二斑叶螨各螨态的捕食偏好性、捕食功能反应及自身干扰反应。结果表明,草栖钝绥螨对二斑叶螨幼螨和第1若螨具有嗜食性,对其捕食选择系数分别为2.22和1.27,均大于1.00,对二斑叶螨卵、第2若螨和雌成螨捕食选择系数分别为0.61、0.68和0.22,均小于1.00。在不同温度条件下,草栖钝绥螨对二斑叶螨各螨态的捕食功能反应均符合HollingⅡ型;在19～31℃范围内,草栖钝绥螨对二斑叶螨各螨态的瞬时攻击率、最大日捕食量和捕食能力均随着温度升高呈先升高后降低的趋势,在28℃时达到最大值;而草栖钝绥螨对二斑叶螨各螨态的处理时间随着温度升高呈先缩短后延迟的趋势,在28℃下处理时间最短。在相同温度下,草栖钝绥螨对二斑叶螨卵、幼螨和第1若螨的捕食作用较强。在有限的捕食空间和二斑叶螨密度固定的条件下,草栖钝绥螨单头捕食量和捕食作用率随其自身密度的增加而逐渐下降,说...     

Temporal-spatial Filtering in the Preprocessing of Clinical EEG Signals

EEG spot-recording for clinical patients in the bed is always advocated due to easing patients and making them comfortable. But EEG signals recorded in the ward exposed to various noisesand interference is less effective in the EEG analysis. Atemporal-spatial filter is designed for noise removing of clinical EEG signals, the first filtering is temporal filtering using band-pass filter, and the second filtering is spatial filtering with ICA-based spatial filter. Experimental results indicate that various noises and interferences, such as power interference, blinking, eyes movements, muscle moments, ECG artifacts, etc., are removed effectively, individually or simultaneously.     

Influence of MUD on the System Capability of CDMA

In CDMA communication system, the relativities between users result in Multiple Access Interference (MAI). With the increase of users, MAI becomes the main jam of broadband CDMA communication system. Multi-User Detection (MUD) is the most important technology of anti-jamming in the broadband CDMA communication system, which can eliminates MAI effectively by using the information of all user signals to detection single. This paper analyze the expression of system capability without MUD and with MUD, and puts forward the way to increase system capability by using MUD. Then the influence of MUD on CDMA system capability is discussed by MATLAB emulation. Basing analysis and emulation, the conclusion is got: the higher the MUD efficiency, the better the improvement of CDMA system capability; under the same MUD efficiency, the lower data rate, the smaller inter-cell interference, the lower the ratio of bit energy to power spectrum, the better the improvement of CDMA system capability.     

变压器局部放电在线监测技术分析

阐述了变压器局部放电在线监测的原理、现状以及在线监测方案如何选择，分析了变压器局部放电在线监测中的各种干扰及其抑制方法并提出了实际监测中分层式抑制干扰的模式，探析了目前在线监测局部放电所存在的问题。