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61.
62.
为建立倍他米松(BET)ELISA检测方法,先使BET与琥珀酸酐反应,再采用活化酯法与牛血清白蛋白(BSA)偶联制备免疫原;其次,利用免疫动物获得多克隆抗体,经Sephrose 4B-proteinA方法对抗体进行纯化;最后,建立倍他米松ELISA检测方法。结果表明,免疫抗原偶联成功,获得了亲和力较高的多克隆抗血清,间接竞争ELISA测定其滴度为102 400、IC15为6.60×10-5 ng/mL和IC50为0.97ng/mL。该方法适用于残留在动物性食品中的BET现场大批量检测。 相似文献
63.
Miguel Mellado Lucía M. Treviño María I. Chavez Francisco G. Véliz Ulises Macías-Cruz Leonel Avendaño-Reyes José E. García 《Reproduction in domestic animals》2023,58(1):39-47
The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding. 相似文献
64.
65.
对砌石拱坝的加高倍厚中新、老坝体结构面,用有限元软件ANSYS结合接触理论进行计算,并分析结果得出:温升、水压力和淤沙压力使结合面上的接触压力增大,从而接触摩擦应力较大,有利于结合面的结合,而结合面在温降的作用下有脱离的趋势。 相似文献
66.
In order to analyze the reason why the Spiral Up-flow Reactor system has excellent removal effect of COD, N and P, the authors use PHOENICS, a computational fluid dynamics program, to investigate the hydraulic characteristics and flow model of the anaerobic part of Spiral uP-flow reactor. The result shows that the fluid in the reactor unit flowed upward by the spiral way, and that flow pattern was combined by plug flow and complete-mix flow, and that the percentage of plug flow was about sixty-five percent. Spiral Up-flow Reactor units the Characteristics of PFR and CSTR, which makes it work well. 相似文献
67.
裸子植物体细胞胚胎发生和人工种子的研究 总被引:8,自引:0,他引:8
该文介绍了一种作为森林种子的大规模增值的有效方法--裸子植物体细胞胚胎发生和人工种子的研制。其内容包括:(1)胚性愈伤组织的诱导与保存;(2)影响体胚发生的因素;(3)体胚的发育与成熟;(4)大规模培养体胚;(5)成熟体胚的干化、包理与人工种子生产;(6)体胚的萌发和转换。最后对人工种子的应用前景进行了讨论。 相似文献
68.
The processing characteristics of the front bumper bracket are analyzed,and a reasonable process scheme is worked out.The three-dimensional sheet metal forming analysis software PAM-STAMP are adopted to simulate the bracket's drawing process.Then the surface of die is proved fine through analyzing the FLD diagrams and the forming results.An economical and optimized die structure is decided,and a complete set of dies of the bracket is designed.The spacing block is added in the sizing mold,which control the size precision of end products and assembling columns actively,and finally the certified products.The material utility and the labor productivity are raised,the production cost is saved,and the mold design quality and the efficiency are improved effectively. 相似文献
69.
Boiler is an important facility in power plant , the safety and economy of unit is affected by it. Studying the dynamic of boiler can improve the knowledge about boiler , which is important for. Features of MMS are introduced based on MMS simulation setting, and the real time capable module and non real time capable module are compared from principle. Real time capable dynamic model of boiler and non real time capable dynamic model of boiler of 330 MW unit are established . The experimental results show that the dynamic process of two type model accords with the mechanism of the object , at the same time, the validity of real time capable module is discussed . 相似文献
70.
HE La-mei~ 《保鲜与加工》2004,(5):57-61
To solve potential scheduling problems in production after renewal of continuous casting process in Vanadium-extracting and Steel-making Plant of Panzhihua Iron and Steel Corporation (PISCO), how to model and simulate logistics system in steel-making processhas been discussed. A series of simulatingtests for logistics system in steel-making processhave been conducted based on inputs of presentsteel-making processof PISCO. In the tests, cases of regular schedule case and cases with repairing converter have also been regarded as essential simulation condition. It has been shown that three casters can not start at same time in order to ensure continuous hot metal supply for casters when three kinds of steel are made. It has also been shown that the simulation system for logistics system in steel-making processis valid and can be used for simulating logisticsin upcoming 100% continuous casting process of PISCO. The better schedule scheme of production and reasonable match of converter-caster in time and capacity can be achieved with the help of using simulation approach. 相似文献