Prevention of isinglass on the chronic atrophic gastritis in rats and its mechanism

AIM: To evaluate the effect of isinglass on chronic atrophic gastritis(CAG) in rats and its mechanism. METHODS: An animal model of CAG in accordance with the previous experience of combined administration of 60% ethanol, 20 mmol/L sodium deoxycholate and 0.1% ammonia water was established in SD rats. Isinglass was used as preventive therapy while we were establishing CAG rat model. Finally all the rats were executed and pathologic changes of the gastric mucosa were studied by gross appearance and microscopy and serum epidermal growth factor (EFG) and growth hormone(GH) contents were tested. RESULTS: In each isinglass prevention group, inflammation grade of gastric antrum was less than that in model group (P<0.01) while the mean ratio of the thickness of gastric mucosal gland and muscularis mucosa (L1/L2), the number of gastric glands in 1 mm lengths of mucosal layer in longitudinal sections were much better than those in model group (P<0.01).They were very close to normal control group (P>0.05). The expression of proliferating cell nuclear antigen (PCNA) in gastric mucosa and serum EFG level were higher than those in model group (P<0.01, P<0.05), but serum GH content showed no different between isinglass prevention group and model group. CONCLUSION: Isinglass preventes the gastric mucosal atrophy in the CAG model. Its mechanism may be related to the effects of decreasing the gastric mucosal damage， promoting the cell proliferation and increasing of internal EFG secretion.     

Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro

AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.     

猪繁殖与呼吸综合征病毒感染对猪肺泡巨噬细胞外源性抗原加工递呈相关分子和共刺激分子的影响

采用定量竞争PCR技术对猪繁殖与呼吸综合征病毒（PRRSV）感染后不同时相猪肺泡巨噬细胞（PAM）中外源性抗原加工递呈相关分子SLA-DR、Ii链和SLA-DM分子及共刺激分子CD40、CD80、CD86的mRNA转录动态进行了定量检测。结果表明,PRRSV感染后PAM的SLA-DR mRNA转录水平在3、7和14 d下调,低于对照组;感染后3 d,Ii链、SLA-DM和CD40的mRNA转录水平极显著下调（P〈0.01）;CD80和CD86mRNA转录水平也在感染后3 d明显下调（P〈0.05）。用流式细胞术检测PAM表面的SLA-DR抗原的结果表明,在感染后3 d短暂上升,7 d之后一直低于对照组。由此说明,PRRSV感染早期对猪肺泡巨噬细胞的外源性抗原加工和递呈功能产生显著影响,导致其外源性抗原递呈功能下降,进而影响机体的体液免疫应答。     

Immunopathology of the eye: Purification of canine retinal “S” antigen

Canine retinal S antigen has been purified to study the retinal progressive atrophy of the dog. The purified antigen will be used to detect, by the ELISA technique, specific autoantibody in dogs with ocular diseases.     

我国牛群中牛病毒性腹泻病毒流行的Meta分析

为全面评估我国牛群中牛病毒性腹泻病毒（bovine viral diarrhea virus,BVDV）病原流行率对该病毒感染的防控，在中国知网（CNKI）、万方、维普、PubMed和ScienceDirect 5个数据库中，检索截止到2021年9月1日发表的关于中国牛群BVDV流行情况的文献，并进行系统评价和Meta分析。共筛选出93篇关于BVDV病原学检测的研究论文纳入Meta分析中。分析结果显示：我国牛群中BVDV病原（抗原和核酸）流行率为9.8%(95%CI:7.8,11.9)，其中BVDV抗原流行率为3.1%(95%CI:2.1,4.4),BVDV核酸流行率为19.5%(95%CI:16.0,23.3)。各省间比较发现，吉林流行率最高，为26.3%(95%CI:24.3,28.4)，其次是湖北和福建省。进一步开展亚组分析和Meta回归分析的结果显示，品种（牦牛vs奶牛：比值比（odds ratio,OR）=1.37,95%CI:1.05～1.79）、饲养模式（散养vs规模化：OR=1.41,95%CI:1.08～1.85）、诊断方法（RT-PCR vs ELISA:OR=1....     

空肠弯曲菌共同抗原单克隆抗体的研制及其在细菌检验中的应用 Ⅱ.杂交瘤细胞株的建立及特性鉴定

应用B-淋巴细胞杂交瘤技术,建立了5株稳定分泌抗空肠弯曲菌共同抗原(CA)单克隆抗体(McAb)的杂交瘤细胞株(4A7,9D6、9C2、11A1、11D4)。在5株杂交瘤分泌的McAb中,有4株(4A7、9D6、11A1、11D4)针对CA中的耐热抗原成分,对其中4A7、9D6两株分泌的McAb进行免疫印迹分析表明,其与CA中大约为7.7×104u的蛋白移行带出现特异反应;一株(9C2)针对CA中的不耐热抗原成分。对杂交瘤细胞的核型分析表明,其染色体数在93-108之间。体外传代4个月,仍保持稳定分泌抗体的特性。这种McAb具有较强的特异性,除与幽门弯曲菌出现弱阳性反应外,与参试的其它31种非弯曲菌属的细菌均为阴性反应。对不同地区、不同来源的516株空肠弯曲菌的鉴定表明,制备的McAb与现有空肠弯曲菌均出现阳性反应,与常规鉴定法的符合率为100％。     

Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIα and IIβ genes of Japanese flounder Paralichthys olivaceus

ABSTRACT:   The nucleotide sequences of Japanese flounder Paralichthys olivaceus , major histocompatibility complex (MHC) cDNA, classical MHC class Iα, non-classical MHC class Iβ, MHC class IIα and IIβ, were determined. The domain structures and antigen binding motifs of vertebrate MHC are conserved in the Japanese flounder MHC. A phylogenetic analysis supports the classification of these genes into class I and class II MHC. Classical MHC class Iα was ubiquitously expressed, whereas the non-classical MHC class Iβ was expressed mainly in lymphoid organs, gills, intestine and stomach. The MHC classes IIα and IIβ were also ubiquitously expressed.     

呋喃妥因代谢物化学发光酶联免疫检测方法的研究

对呋喃妥因代谢物1-氨基乙内酰脲(AHD)进行半抗原改造,采用活化酯法将半抗原与卵清蛋白(OVA)偶联为包被原,与标准品竞争AHD单克隆抗体的抗原结合位点,加入辣根过氧化物酶标记羊抗鼠二抗,建立了AHD间接竞争化学发光酶联免疫(CLEIA)检测法,并对化学发光液、包被原与抗体最优稀释度、包被条件、封闭液和竞争时间五项参数进行优化。结果表明:该方法具有良好的特异性,IC50为0.753 ng/m L,在鸡肉组织中的检测限为0.028μg/kg,添加回收率在80.54%~102.64%之间,变异系数均小于10%。与国标中液相色谱-串联质谱法(LC-MS/MS)和我国出入境行业标准中酶联免疫检测法(ELISA)相比,不仅降低了检测限,而且操作简便,为动物源性食品中呋喃妥因代谢物的残留提供了准确、便捷的分析检测手段。     

Identification of novel tumour‐associated antigens in canine mammary gland tumour

Canine mammary gland tumour (MGT) is the most common neoplasm in female dogs and has similar biological characteristics to human MGT. Spontaneous canine MGT is a more attractive clinical model in oncological research than that of the murine experimental model. Tumour‐associated antigens (TAAs), which are produced in tumour cells, are applied as tumour markers, tumour vaccine antigens and molecular targets of therapeutic drugs. In this study, we have primarily identified 13 different TAAs of canine MGT by serological immunoscreening of cDNA expression library. The results of serological mini‐arrays of identified antigens showed that CCDC41 antigen specially reacted with 35% of sera from MGT‐dogs and did not react with control sera. We also found that HSPH1 mRNA expression levels increased significantly in MGT tissues. These findings will contribute to the development of diagnostic technologies and translational target therapies for dogs. Clinical relevance : HSPH1, which is strongly expressed in the tumour tissue, will be a possible vaccine antigen of canine MGT.