金叶含笑叶片基因组DNA提取方法的比较

以金叶含笑叶片为实验材料,分别采用CTAB法、改良CTAB法、简易提取方法、高盐沉淀法和SDS法5种方法提取金叶含笑叶片的基因组DNA,并通过琼脂糖凝胶电泳、紫外分光光度计及ISSR扩增3种方法对所提取的DNA样品进行检测.DNA纯度、含量比较以及扩增结果表明:5种方法中简易提取法和SDS法提取的DNA纯度较高.高盐沉淀法得到的DNA产量较高.5种方法提取的DNA用于ISSR扩增,其扩增结果基本一致.简易提取方法操作简单.省时省力,是用于ISSR扩增的金叶含笑基因组DNA提取的最佳选择.     

整地方式对杉木生长和经济效益的影响

用４种不同整地方式１７ａ的试验资料，分析了整对林木各种生长的影响；用帖现方法，比较了不同整地方式的经济效益。结果表明：整地方式对杉木各种连年生长的量的影响，一般在７－１０ａ消失；对平均高，胸径和单株材积的影响，主要箐面在１０－１２ａ前，在７－１０ａ差异最大；整地对胸径的影响比对高树高和单株积的影响短１－２ａ。     

常绿阔叶林迹地炼山营造杉木林群落演替趋向调查初报

据样地调查，该２５年生杉木人工林群落的植物有４１科８４属１２０种，群落的外貌特征以包括藤本在内的高位芽占绝对优势，叶特征以革质，单叶，中小型叶为主，由于该群落的幼树层以米储和木荷等占优势，若让其自然发展或杉木林采伐后不炼山，并排除其它人为干扰，该群落将向与常绿阔叶林采伐前的群落类型相似的方向演替。     

福建省桐棉松区域栽培试验

初步试验表明：桐棉松能够适应福建省地理生境且具抗逆性，生长迅速；同当地马尾松生长相比，树高生长差异达极显著水平，地径生长差异达显著水平；在海拔800m以下，海拔因子对树高生长有一定影响，但未达显著水平。     

福州地区人工杉木林分蓄积量表的研究

本文提出以林分构成因子——林分胸高总断面积及林分平均高，估计林分蓄积量的办法，选取９种方程求解参数，配合了最优林分蓄积量经验方程，制定出《福州地区人工杉木林分蓄积量表》，对完善我省林业数表的种类、形式和内容，进行了初步尝试。     

三种常用方法防治松干线虫的研究

在病虫害防治过程中切断传染路径是重要的传统防治方法。松干线虫的主要传染媒介是松墨天牛,伐除病死木、熏蒸方法处理残余伐根、诱捕器捕杀天牛成虫3种方法相结合,处理染病林分,并和没有进行处理的林分进行比较,试验表明,染病个体植株数明显下降,松墨天牛虫口密度大幅度降低。基于这样的思路,设计了试验方案,收集大量数据,并进行数据处理分析,得出结论,为松干线虫的防治提供理论依据。     

Intestinal absorption of forsythoside A in different compositions of Shuang-Huang-Lian

Shuang-Huang-Lian (SHL), a traditional Chinese formula containing Lonicerae japonicae flos (LJF), Scutellariae radix (SR) and Forsythiae fructus (FF), is commonly used to treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. Forsythoside A is one of the main active ingredients in Forsythiae fructus, a key herb in SHL. In the present study, effects of different compositions in SHL on the intestinal absorption of forsythoside A were investigated. The observations from in situ intestinal circulation model showed that A/%(h^− 1) of forsythoside A in FF + LSF, FF + SR and SHL were all reduced greatly compared with that in FF. However, in pharmacokinetics study, C_max and AUC_0 → 1440 of forsythoside A all increased and T_1/2 prolonged in SHL, FF + LJF and FF + SR compared with FF. The results indicated that the different compositions of SHL decreased absorption but increased bioavailability of forsythoside A, which may be related to its metabolism inhibited in intestine or liver.     

Effects of light,macronutrients, and sucrose on germination and development of the endangered fern Adiantum reniforme var. sinense (Adiantaceae)

Effects of light, macronutrients strength of Murashige and Skoog (MS) (1962), and sucrose in the culture medium on spore germination and gametophyte development of the endangered fern Adiantum reniforme var. sinense were investigated. The presence of light was found to be essential for both spore germination and gametophyte growth. Moreover, a medium consisting of 1/4 MS with 15 g/l sucrose was optimal for spore germination and early gametophyte development; whereas, MS medium with 30 g/l sucrose was optimal for further gametophyte development. Increasing amounts of sucrose (45–60 g/l) in the medium delayed gametophyte growth and development. Additionally, sporophyte formation and early growth of gametophytes in a medium consisting of clay and peat (v/v = 1:2) was higher than those in a medium consisting of pure river sand. These findings indicated that requirements for nutrients for spore germination and early gametophyte development of A. reniforme var. sinese were relatively low, but these increased with further gametophyte development, formation and growth of sporophytes.     

毒死蜱和甲氰菊酯对家蚕毒性与安全评价研究

通过食下毒叶法和接触法测定了毒死蜱和甲氰菊酯对家蚕的毒性。结果表明:食下毒叶法对2龄起蚕的LC50(48h)分别为:毒死蜱2.3322mg/kg桑叶和甲氰菊酯0.1522mg/kg桑叶,其浸渍桑叶的药液浓度分别为4.6622mg/L和0.3044mg/L。接触法中毒死蜱和甲氰菊酯对2龄起蚕的LC50值(24h)分别为:6.32μg/cm2和0.92μg/cm2。毒死蜱和甲氰菊酯的毒性比都<1,对家蚕的毒性为高毒。     

黔东北武陵山区白线树蛙特异性兼佛顶山国家级自然保护区新纪录

在贵州省佛顶山国家级自然保护区发现一白线树蛙(Rhacophorus leucofasciatus).该标本与梵净山国家级自然保护区发现的白线树蛙标本具有稳定的一致性特征,与广西金秀白线树蛙(Rhacophorus leucofasciatusLiu et Hu)模式标本特征相似,同时存在明显差别.表现为:佛顶山标本指间蹼不发达,外侧2指具半蹼,第三指外侧及第四指内侧蹼未达吸盘基部,第二、三指指间蹼为1/3蹼,内侧2指即第一、二指指间有蹼迹;上臂背面米黄色;白线树蛙模式标本指间蹼发达,外侧3指间具全蹼,第二指外侧及第四指内侧蹼达吸盘基部,内侧2指指间蹼少;上臂背面为白色.佛顶山与梵净山同属于黔东北武陵山区,该物种为佛顶山国家级自然保护区两栖动物新纪录,地区特异性显著.