烟草钾转运体基因NtHAK1的克隆及表达模式分析

采用RT-PCR的方法,结合3'RACE与5'RACE,从普通烟草(Nicotiana tabacum)品种K326中克隆出烟草K+转运体基因NtHAK1的全长cDNA序列.该序列长度为2016bp,编码378个氨基酸,其分子量为42.27kDa,等电点7.2.生物信息学分析表明,该蛋白具有6个跨膜区,含有K+转运蛋白...     

沙漠地区风沙活动特征——以中国科学院风沙观测场为例

运用中国科学院风沙科学观测场的实测资料,对腾格里沙漠风沙环境特征进行初步分析,内容包括起沙风、风沙活动强度、风沙流结构和沙漠边界层的风速廓线.该地区的起沙风以6～8 m/s为主,占总起沙风的71.63%,其次为8～10 m/s,占19.24%,两者之和占90.87%;大风日数为4天;风向以W-N组风向为主;占全年的53.14%.年输沙势为36.56VU,风能属于低风能环境,单一主风向和单风态风环境.风沙流主要集中在地表的0.1 m,占总输沙量的95.46%.观测场近地层厚度大于50 m.     

Influence of Water Stress on the Chemical Composition of Seeds of Two Lupins (Lupinus albus and Lupinus mutabilis)

We have performed chemical proximate analysis and determined the carbohydrate profile of mature seeds of Lupinus albus, cv. Rio Maior and Lupinus mutabilis, cv. Potosi plants, either well watered (ww) or subjected to a water-stress period (imposed from the 15th to 35th day after anthesis). Protein content from lupin seeds was 450 and 320 g kg⁻¹ of seed on dry weight basis, for ww plants, respectively, of L. albus and L. mutabilis. Water stress did not affect protein content. The oil content of ww plants was about 120 g kg⁻¹ of seed dry weight for L. albus and 180 g kg⁻¹ of seed dry weight for L. mutabilis. Water stress reduced those values of half. The sugar content was about 90 g kg⁻¹ for L. albus and 50 g kg⁻¹ of seed dry weight for L. mutabilis, in ww plants. Water stress led to an increase in sugar content to 200 and 130 g kg⁻¹ of seed dry weight, respectively, for L. albus and L. mutabilis. The α-galactosides amounted to 70 % of the total sugars (raffinose being 30 % and stachyose, 40 %) and sucrose was about 20 % of the total sugars. Water stress increased total carbohydrates and increased the sucrose/α-galactoside ratio, although reducing raffinose content and increasing sucrose content.     

搅拌型酸奶流变学特性的研究

以市场上流行的6种搅拌型酸奶为研究对象,对其流变学特性及质构特性进行了初步的研究.结果表明:6种酸乳的剪切应力值在升速和降速过程中都能形成触变环,表观粘度在升速过程中随剪切应力的增大而逐渐降低,降速过程中随着剪切速率的降低而升高;酸奶在16 r/min的剪切速率下,转速和相应的表观粘度符合幂率定律,进一步发现搅拌型酸奶具有时间触变性,是一种正触变性流体.由触变环的面积可知,6种搅拌型酸奶的触变性大小:S5＞S4＞S2＞S1＞S3＞S6,触变性的大小对样品的口感有非常重要的影响.此外,酸奶的流变学分析得出口感较好的酸奶粘度值应在540.56～674.25 mpa·s范围内;酸奶质构分析发现口感较好的酸奶硬度在33.9～39.9 g范围内.     

微机绘制索道侧型图的研究

所编制的 BASIC 语言程序,能根据给定条件数据自动绘制单跨或多跨索道侧型图,并标注主要参数和有关尺寸。使用本软件绘制索道侧型图,其功效高,操作简便,运行速度快,绘图精确,通用性好。     

绘制天然河道水面曲线的图解计算法

本文根据流量模数的倒数曲线法,利用合轴图及数值叠加规则,提出了一种适用于山区河道的图解计算方法,本法解算过程较为直观,便于检查正误,计算精度较高,特别是在需要绘制一系列流量水面曲线的情况下,可加快计算的进度。     

作物根层(0～100cm)土壤剖面残留无机态氮研究报告之二Nmin含量与小麦产量的关系

1984～1986年在部分地区进行的 Nmin(0～100cm)与小麦产量相关性研究说明,Nmin 含量与小麦产量的相关性高,可作为推荐施用氮肥的指标。     

The Diurnal Variation of Urea in Cow’s Milk and how Milk Fat Content,Storage and Preservation Affects Analysis by a Flow Injection Technique

Six Swedish Red and White dairy cows, producing 20-39 kg of 4% fat-corrected milk were given a ration balanced in energy and protein. They had access to feed from 05.15 to 09.00 and from 13.00 to 16.30 and were milked at 06.15 and 15.30. The milk was analysed for urea with a FIA technique.There was a significant diurnal variation in milk urea. The highest values were found 3–5 h after the beginning of the morning feeding and the lowest values (down to 60% of the max. values) during late night. Within 1 h after the start of the morning feeding the urea values had increased significantly, but they had decreased within the same time after the start of the afternoon feeding. Since there was a pronounced diurnal variation in the milk fat content, the urea concentration was also recalculated to concentration in the water phase of the milk. It was higher in that phase, but the pattern of the diurnal variation was not changed significantly. However, analyses on milk with a very high fat content may give misleading results.There were no important differences in the milk urea concentration of different udder quarters. When calculated as concentration in the water phase of the milk, no differences in urea concentration were found between the beginning and the end of milking. The analytical method had a good precision (coefficient of variation max. 3%). The milk urea concentration was not changed significantly after storage during 10 days at 4°C when no preservative was added; but after 17 days the milk had turned sour and the urea value had increased. When a preservative (bronopole) was added the urea concentration remained unchanged during 17 days. Deepfreezing did not influence the urea concentration.     

渭河中游全新世晚期古洪水沉积物特征

通过野外实地调查,在渭河中游咸阳市南寺村一级阶地的全新世黄土-古土壤剖面,发现了含有波状水平层理的古洪水滞流沉积层,对所采集的全新世地层样品进行了粒度、磁化率、烧失量、CaCO3和石英颗粒微结构表面特征分析,表明古洪水滞流沉积物（SWD）平均粒径小,磁化率值低,CaCO₃含量高,石英颗粒表面“V”形坑和三角痕明显等特征,与风成黄土和古土壤差别显著,判定为典型的古洪水滞流沉积物。通过OSL（光释光）测年及地层学对比,确定古洪水SWD记录了渭河中游3 200~2 800 aBP发生的一组3次古洪水事件,正处于全新世大暖期向晚期转变的过渡时期,气候不稳定,降水变率大,引发了连续的特大洪水事件。此研究成果对于揭示区域水文变化对全球气候变化的响应规律具有重要科学意义。